Louis Stokes Laboratories at the National Institutes of Health
This article reveals a model for the complete ESCRT-0 complex which is comprised of Hrs and STAM proteins. This complex sorts ubiquitinated cell surface receptors to lysosomes for degradation. A 1:1 stoichiometry was determined by using Wyatt multi-angle light scattering (DAWN HELEOS) and quasi-elastic light scattering (QELS) instrumentation along with an Optilab rEX differential refractometer and ASTRA V software. A monodisperse complex was confirmed with static light scattering measurements of 140 kDa and a hydrodynamic radius of 7.9 nm.
Ren, X.; Kloer, D. P.; Kim, Y. C.; Ghirlando, R.; Saidi, L. F.; Hummer, G.; Hurley, J. H. Structure 2009, 17, 406-416. DOI: 10.1016/j.str.2009.01.012