High-throughput assays for promiscuous inhibitors

During the high-throughput screening of compound libraries, false positive results can be generated because some compounds form aggregates that nonspecifically inhibit receptors. These compound aggregates are readily detectable by optical methods such as light scattering. This article describes a system that measures dynamic light scattering, using the same conditions as for HTS, allowing the identification of false positives very early in the screening process.

Feng, B. Y.; Shelat, A.; Doman, T. N.; Guy, R. K.; Shoichet, B. K. Nature Chemical Biology  2005, 1, 146-148. doi:10.1038/nchembio718