Wyatt Technology

AF4-MALS: A Powerful Tool for the Characterization of Biomacromolecules and Colloidal Particles

 

Presented Live: March 16, 2016

Speaker: Professor Lars Nilsson, Lund University

Biomolecules can cover a wide range of sizes, from small peptides of 103 Da to aggregates of antibodies having sizes of up to hundreds of nanometers. To facilitate characterization of molecules in a complex matrix, it is often of benefit to separate the different species prior to investigations. Asymmetric flow field-flow fractionation (AF4) is one instrumental technique that offers the possibility to physically separate biomolecules across a wide size range. AF4 can be used in combination with various types of detectors depending on the information required and becomes especially power when combined with multi-angle light scattering (MALS) enabling the determination of molecular weight and size. In this presentation examples are shown for different biomolecules, lipid vesicles, biopolymers and nanoparticles as well as what to consider when size characterizing such compounds.

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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