Wyatt Technology

Combining Size and Molar Mass Measurements of Proteins and Biomolecules Using the DynaPro NanoStar

 

Presented Live: February 18, 2010

Static and dynamic light scattering (DLS) can be used either in standalone (unfractionated, batch) mode or on-line coupled with a separation technique, such as size exclusion chromatography (SEC) or field-flow fractionation (FFF).

Topics Covered

  • Application examples for simultaneously measuring molecular size and absolute molecular weight of proteins and biomolecules using the DynaPro NanoStar
  • Analysis of thermal stability of protein solutions (protein unfolding and aggregation)
  • How to integrate DLS with MALS in a fractionation system, as well as how the resulting combined size and molar mass measurements can reveal unique insight into protein and biomolecule conformation and aggregation

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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