Wyatt Technology

Small, but bright: µSEC-MALS adds light scattering to UHP-SEC for biophysical characterization of biotherapeutics

 

Speakers:
Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. Michelle Chen, Director of Analytical Services, Wyatt Technology Corp.
Mark Haverick, Senior Scientist, Merck & Co Inc.

UHP-SEC-MALS combines the high speed, high resolution, and low sample consumption of UHP-SEC separation with online multi-angle light scattering (MALS) analysis. MALS is the recognized, indispensable method for obtaining absolute molecular weight and size, independently of column retention time. In this webinar we present the key features of UHP-SEC as well as a MALS detector optimized to couple with it, creating a “µSEC-MALS” system for protein and synthetic polymer characterization.

Case studies will illustrate the applications of µSEC-MALS in the analysis and characterization of biopharmaceuticals. In particular we will discuss some of the challenges of bridging analytical-scale SEC-MALS to µSEC-MALS, as well as bridging between different UHP-SEC elution conditions.

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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