The Eclipse AF4 solves the most difficult separation, fractionation and sizing tasks in the macromolecular and colloidal arenas. The advantage of AF4 over traditional SEC column chromatography is the ability to separate both soluable and colloidal components over a wide size range as well as sensitive and "sticky" samples (no stationary phase). Its resolution is similar to sedimentation equilibrium, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
The Eclipse AF4 is invaluable and indispensable in key areas of research and development in molecular biology, nanotechnology, and environmental analyses, including the separation and characterization of proteins and aggregates, liposomes, emulsions, viral particles, polysaccharides, nanoparticles, polymer latex particles, colloidal soil suspensions, humic substances, chemical mechanical polishing slurries (CMP), and many more.
Separates both soluble and colloidal components over a wide size range; especially unmatched powers in sub-micrometer range
Large dynamic range: from a few nanometers to a few micrometers
Rapid analysis: typically 10 to 30 minutes
Absence of shearing forces (no stationary phase; separation takes part in separation channel)
Ultra-high resolution separations, comparable to ultra centrifugation
Simplifies analysis by eliminating many time-consuming sample preparation steps
Adjustable and flexible separation ability: very efficient for complex samples
Direct injection: minimal sample preparation necessary
Interfaces with modern analytical methods like MALS, MALDI-TOF, etc.
Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ICP-MS, ELISA etc.)
The Eclipse is the world's most advanced AF4 system
Fully automated, software controlled system with only one pump to generate detector flow, cross flow and injection flow. This innovative concept to generate all three flow streams from one main flow has been a breakthrough for the AF4 method!
One chassis contains all necessary electronic and fluid components, designed to stack with Wyatt Technology detectors (DAWN® , Optilab® , ViscoStar)
Eclipse Chassis can be ordered in three versions: for aqueous solvents (biocompatible), for organic solvents and in a metal-free version
The Eclipse integrates into various market-leading HPLC packages, such as Agilent, Dionex and Shimadzu.
Independent, continuous measurement of the cross-flow and injection flow rate, which allows software controlled check on the system performance
Motor driven, software controlled needle valves for focus balance and injection flow rate control
Proprietary pressure sensor without dead-volume provides continuous read-out of channel pressure
High performance electronic controller inside the chassis (PLC programmable logic controller) with TCP/IP communication to PC
Optimized layout of fluid connections minimizes tubing length and ensures easier serviceability
Eclipse chassis and electronics are protected against fluid damage through customized drip-pan with leak sensor
LCD displays system status information
CE classification surpasses laboratory standards for safety and emission and reaches industry grade
The Eclipse is certified by TÜV Rheinland
Eclipse AF4 Channel Design:
The Eclipse offers the most versatile separation channel technology. There are five different channel types plus the hollow-fiber-cartridge available to accommodate different requirements for sample load, analysis time, and resolution, as well as a wide choice of spacers and pre-cut membranes for all channel types. All channels have in common that the flow path is metall free, making them inert even in harsh conditions. The frit-inlet channel is shown with a cross-section displaying from bottom to top the lower PEEK-block, the frit, membrane and polycarbonate upper plate which is placed in an aluminum frame. The frit supporting the membrane is made from stainless steel, which is the most robust choice. As an option a metal free ceramic frit can be installed. All channels are easy to maintain. Replacing a membrane or cleaning the channel parts is straightforward, with just a few screws to tighten during the reassembly process.
Mini Channel (MC, 110 mm length)
Short Channel (SC 145 mm length)
Long Channel (LC, 275 mm length)
Semi-Preparative Channel (SP, 45 mm width, 275 mm length)
Frit-inlet Channel (Fl-AF4)
Hollow-Fiber FFF Channel (HF5)
Eclipse Complete Solutions
Integration with Wyatt Technology instruments and market-leading HPLC packages:
The Eclipse separation system is designed to integrate with Wyatt Technology’s range of unique detectors, ensuring highest sensitivity and optimum baseline quality.
The Eclipse uses either Agilent, Shimadzu, or Dionex chromatography components. The users can stay with their HPLC software platform and run the Eclipse with the familiar interface they already know.
Only one pump needed to generate detector flow, cross flow, and injector flow by splitting the main flow delivered through the HPLC pump. Using only one pump results in a much improved signal-to-noise ratio because pump pulsations are reduced and operation is much easier.
Please visit our on-line Bibliography and type in the keyword "fractionation" to view peer reviewed papers using this combined FFF/MALS technology.
Separation size range:
1 nm to 1 µm, depending on specific sample properties, in normal elution mode. Up to 10 µm in steric mode.
Cross flow rates:
Channel dependent :
0.04 to 3.3 mL/min standard
0.07 to 6.6 mL/min
0.1 to 8.3 mL/min
Maximum total flow rate:
10 mL/min = cross flow plus detector flow rate
Maximum channel pressure:
30 bar. Software overpressure shut-down
Set of spacers with 250, 350 and 490 µm; each thickness comes in two different channel widths
Polyether Sulfone: 1, 3, 5, 10, and 30 kDa cut off
Cellulose Triacetate: 10 kDa cut off
Regenerated cellulose: 5, 10, and 30 kDa cut off
Micro MC: 114 mm effective length (e.l.)
Short SC: 153 mm e.l.
Long LC: 246 mm e.l.
Semi-Prep SP: 246 mm e.l.
MC, SC, LC maximum spacer width 22 mm
SP maximum spacer width 44 mm
Rated for aqueous solvents and alcohols
Aqueous channel: PEEK, aluminum cover with polycarbonate insert, Viton, stainless steel
Rated for organic solvents, e.g. THF, toluene, etc.
Stainless steel tubing
Additional vapor sensor with interlock output
Organic channel: PEEK, Kalrez, aluminum, stainless steel
SEC switch option:
Software-controlled valves change flow path from AF4 channels to SEC column, rinse and waste
Front panel LCD
Autoinject In, Analog In
Autoinject Out, Analog Out, Detector Zero, Start Pump, Agilent pump interlock (organic version only)
Integrated in Agilent Chemstation®, Dionex Chromeleon®, and Shimadzu’s LC Solution®
Warranty: All Wyatt instruments are guaranteed against fabrication defects for twelve months.
Should any unit become defective due to normal use within the warranty period, we will repair or replace it at no charge.
Wyatt Technology has a policy of continual improvement. Specifications subject to change without notice.