The world’s most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.
18-angle light scattering detector for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution. The DAWN® HELEOS™ II may be used in batch mode (off-line) or connected on-line to an HPLC/HPSEC/AFFF, etc.. The DAWN® HELEOS™ II utilizes a 120mW solid-state laser operating at 658 nm and also has on-board digital signal processing hardware for up to four external devices such as RI and/or UV detectors. Also contains an analog output from the 90° detector for interfacing to strip chart recorders. The DAWN® HELEOS™ II has a gorgeous 64,000 color LCD display, thermostatic control options, and depolarization options.
The DAWN may also be operated up to 210 degrees and interfaces with high temperature GPC systems such as the GPC-IR system from Polymer Char. A picture of the the high temperature DAWN interface to the Polymer Char GPC may be veiwed at: Photo
Wyatt Technology is the world leader in providing the most advanced macromolecular and nanoparticle characterization tools. These instruments and software may be used to determine the absolute molecular weight and or size of macromolecules and nanoparticles and include: multi-angle light scattering, dynamic light scattering, high through-put dynamic light scattering, field flow fractionation, refractive index and viscometry detection.
Light Source:
120 mW GaAs linearly polarized laser;
100 mW delivery to the cell
Laser Wavelength:
658 nm
Laser Lifetime:
>10,000 hours
Detectors:
18 angles
Detector type:
Custom-designed, ultra-quiet hybrid photodiodes
with built-in FET transimpedance amplifiers single
photon counting module for QELS option
Detector Resolution:
24 bit
Mobile phase compatibility:
All-solvent compatible (aqueous and organic)
Instrument Options:
Ambient; Ultra-High (UHT) to 210°C;
Heated/Cooled (HC) –15°C to 150°C;
Embedded Quasi-Elastic Light Scattering (QELS)
Embedded COMET ultrasonic flow cell cleaning system
Flow Cell Options:
Fused Silica for solvent refractive index less than 1.50
F2 for solvent refractive index above 1.50
Scattering Volume:
0.07 μL
Signal Processing:
On chip sinc3 filter with proprietary dust rejection filter
Analog Inputs:
4 differential analog inputs with
24 bit resolution Input range -10V to 10V
Analog Outputs:
2 analog outputs from user selectable
measurements channels -10V to 10V
Other Inputs/Outputs:
Alarm in, Auto inject contact closure, Recycle in,
Alarm retransmit, Recycle valve power
Molar Mass Range:
< 103 to 109 g/mole (Daltons)*
Molecular Size Range:
≈ 10 to 500 nm†
Computer Interface:
Ethernet and USB 2.0 interface Processed Data
Transmission Rate:
Software selectable from 36.6 Hz to infinity
Front Panel Display:
162.5mm 16-bit high resolution LCD display with
Graphical User Interface
Power:
400 W maximum, 200 W typical AC Voltage:
90–250V @ 50–60 Hz, universal power input
Specifications subject to change without notice.
* depending on dn/dc, the sample concentration, and chromatography conditions, this is typical.
† depending on the sample, this is typical.
Wyatt Technology is the world leader in providing the most advanced macromolecular and nanoparticle characterization tools. These instruments and software may be used to determine the absolute molecular weight and or size of macromolecules and nanoparticles and include: multi-angle light scattering, dynamic light scattering, high through-put dynamic light scattering, field flow fractionation, refractive index, zeta potential and viscometry detection.
