The most widely used dynamic light scattering instrument.
The DynaPro NanoStar from Wyatt Technology is a brand new, Dynamic Light Scattering (DLS) instrument that is used for the analysis of protein solutions, promiscuous inhibitors, buffers or other products in solution. New specially designed cuvettes allow one to use down to 1 µL of sample! The NanoStar is empowered with more than twice the signal-to-noise of its predecessor, and has replaced virtually every major component in order to make it a full-fledged member of Wyatt's family of instruments and part of the easy-to-use DYNAMICS® software.
Since its inception in the early 1990's, the Protein Solutions™ DynaPro™ DLS instrument began appearing in protein crystallization and characterization labs throughout the world - the first DLS (Dynamic Light Scattering) technology meeting the scientific community's need to measure small volumes of proteins under very dilute solution conditions. The DynaPro quickly earned acceptance on a global basis, and has since become an indispensable tool for biomolecular sizing and characterization.
The new instrument has a TCP/IP interface, so it can be put on a laboratory network instantly and operated from anywhere with an Internet connection. The NanoStar comes with a double density correlator installed. The DD correlator has 512 bins from 100nsec to 1 hour. Instead of having 16 bins in the first stage and 8 in each subsequent stage, it has 32 in the first stage and 16 in the higher stages.
In order to insure that the NanoStar never goes out of range, it comes equipped with automatic attentution over five orders of magnitude. Its air-launched laser, coupled with proprietary optics for higher sensitivity, make single mode optical fiber technology obsolete. Refinements have been made to the temperature control systems for improved response time, with an overall temperature range of -15C to +150C, and the ability to couple with Wyatt's on-line multi-angle light scattering systems for enhanced resolution are also standard parts of the package.
Finally, a dedicated Static Light Scattering detector has been added to the NanoStar so that it can measure not only hydrodynamic radii, but absolute molecular weights as well.
Size Range (Radius - nm) 0.2 to 2500
Minimum Lysozyme 0.1 Concentration (mg/ml)*
Scattering Angle (°) 90
Laser Wavelength (nm) 658 (785nm custom)
Laser Power (mW) 10-100 (Programmable)
Min. Sample Volumes (μl) 1μl standard cuvette
Cuvette Materials Quartz Cuvette is
standard.
Optional holder for disposable
plastic cuvettes (DLS operation only)
Number of Samples 1
Temperature Range (ºC) -15° to 150° †
Dry Nitrogen bleed with pressure sensor
(for operation below 20°C) Correlator (100 nsec sampling time in 512 channels a multi-tau layout)
Data Acquisition Time (s) 1 to 3600
Read Time (per well average range) N/A
Dimensions (W x H x D in mm) 357 x 175 x 595
Weight (kg) 13.5
PC ports Ethernet connectivity
Optical Fiber Multi-Mode
Static Scattering Molecular 300 Da to 106 Da Weight Range (concentration dependent)
Specifications subject to change without notice.
* 14 kDa Lysozyme, PBS, 100 seconds data acquisition
† Absolute accuracy of +/– 0.3ºC.
Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution. These tools include: in-line multi-angle static light scattering, high-throughput dynamic light scattering, differential refractometry, electrophoretic mobility, differential viscosity, field flow fractionation and automated composition gradients.
