Absolute Molar Mass
The DynaPro Titan and Titan TC can determine the absolute molar mass of isotropic scatterers (such as proteins) by performing Classical or Static Light Scattering measurements at a single angle. When measured under dilute conditions, when the 2nd Virial Coefficient is known or known to be negligible, and when the refractive index increment is known a single point measurement of the average intensity at a known concentration allows measurement of the absolute molecular weight.
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When measuring particles that scatter non-isotropically, when the particle aggregates with changing concentration, or when the 2nd Virial coefficient is not neglible we suggest performing the experiment with our multi-angle light scattering instruments.
