It’s the intuitive software interface for your Möbiuζ and DynaPro instruments: The Plate Reader, the NanoStar and the Titan. Collect, display and analyze your batch light scattering data from Dynamic Light Scattering (DLS), Phase Analysis Light Scattering (PALS), and Static Light Scattering (SLS) measurements.
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Data Analysis
First, the basics: Size and size distributions by Cumulants and Regularization for DLS data, cumulative size distribution, as well as molar mass determination for SLS data from the NanoStar. Statistical analysis on your measurements with display of mean, minimum, maximum, and standard deviation is provided. But there is more to your results than calculation of size: Dynamics can perform virtually any transition analysis, be it temperature or concentration dependence or any parameter measured or defined by the user. Linear, exponential and sigmoid curve analyses are provided for maximum flexibility.
Size and Size Distributions
Analyze protein and particle sizes using the method of Cumulants or Regularization. Report average radius, diameter and diffusion coefficient as well as the width of the distribution in terms of polydispersity, %polydispersity or polydispersity index (PDI).
In Regularization, size distributions may be viewed as Intensity or Mass distributions and custom data labels may created for each peak.
Cumulative Size Distributions
For a broad size distribution, it is important to control the entire distribution rather than the mean radius alone. Dynamics provides calculation of the percentage of samples below a certain size of particles (either diameter or radius), with values for D10, D50 and D90 (the size of the sample below 10%, 50% and 90% of the entire distribution lies, respectively). T he width of the distribution, defined as (D90-D10)/D50, is calculated as well.
Transition Analysis
Monitor protein melting or unfolding. Determine temperature transitions for your biomolecule. Every molecule behaves differently. That’s why Dynamics provides analysis in terms of the onset transition temperature for aggregating samples, midpoint for samples with a defined end state, and linear analysis for any type of transition.
Similarly, determine the Critical Micelle Concentration (CMC) with our Concentration Dependence analysis. There are no limitations when it comes to analyzing trends for your samples. Any transition can be analyzed with the Custom Parametric Analysis. Select parameters such as pH versus radius or salt concentration versus molar mass.
Even perform statistical analysis for duplicate or triplicate samples, or overlay the analysis curves for multiple samples for all of the analysis types listed above.
Absolute Molar Mass Determination
Measure the absolute molar mass of proteins and small polymers in batch with the dedicated static light scattering detector of the DynaPro NanoStar – simultaneously – while collecting DLS data. Compare the results for molar mass with the hydrodynamic radius to determine molecular conformation and distinguish between aggregation and protein unfolding.
For measurements of the absolute molar mass in chromatography mode or batch measurements of large polymers, we recommend the use of our multi-angle light scattering instruments.
Data Collection
Everything is already set up for the protein scientist. Simply open a new experiment and start recording your data immediately. Data are stored, analyzed and presented in real time. Dynamics features an intuitive 32-bit multi-document graphical user interface. Logically organized, software features are dependent on your specific instrument, showing only the needed features and parameters. At the same time, the software becomes more complex at the right time and situation for data collection, analysis, and presentation. Expandable nodes expose the right level of detail for each measurement. For ease of use, simply click into the appropriate location to bring up desired options in addition to using standard menu items.
Original data
Never lose any original data through preprocessing. All acquired autocorrelation functions are stored and can be readily reprocessed using different, customizable data filters to determine the size distribution and other quantities of interest of your samples. Any processing parameter may be changed post-acquisition.
Automated Data Collection
Unattended operation of your instrument is made possible by using powerful and flexible Event Schedules. Design virtually any sequence of data collection for your instrument, be it temperature or stability studies, plate reader scans or any variation in collection parameters. Wyatt provides an extensive library of Event schedules in the Customer Support Center.
Extensive Solvent Database
Over 200 of the most common solvents and buffers are already included in our standard database. Of course, custom solvents may be added as well.
Automation Interface
An OLE interface is provided for the high-throughput lab using the DynaPro Plate Reader. Interface your instrument with a robotic handler for fully automated plate loading and data collection. Please contact us at info@wyatt.com for details.
Data Viewing
Everything is logically organized: View your results as a table, correlation graph, size distribution or parametric graph with multiple y-axis support. Expandable nodes expose the right level of detail for your measurements, so you can choose to display individual acquisitions, measurements or to overlay complete sets of measurements.
Flexibility
Any choice to display parameters in tables and graphs as well as user selectable units and precision are available. Settings can be saved globally or individually as presets (Standard Operating Procedures). Graph settings are customizable with custom axis displays, ranges, fonts and user-defined peak labeling. Overlay views for autocorrelation functions and sized distributions show reproducibility of repeat measurements or changes in your sample.
Fully Customizable
Add custom solvents and parameters to your data. Custom parameters may be even entered directly in your data table. Even better: Make those a part of your measurement name with the handy Names function!
Variable Data Filter
Outliers due to dust may be filtered out by a number of criteria. Apply your filter to all data, selected data, or even manually mark out individual acquisitions. Filtration criteria may be changed at any time.
Data Export and Reporting
Any display can be exported as a graph or as original data with a simple click of the mouse or by pressing shortcut keys. Results reports are partially customizable.
Helpful Tools
Calculators are included in Dynamics to aid experiment design and evaluation of results. Use the Temperature Ramp Rate Calculator to design your thermal experiments for the DynaPro Plate Reader and NanoStar. The Optimization Calculator helps select the correct acquisition parameters and estimates the radius of your sample from a known molar mass. The Apparent Fraction Calculator determines the relative fraction of two species in equilibrium, such as a monomer-dimer. Additionally, the shape of proteins can be estimated from the Perrin Factor using the Axial Ratio Calculator.
Like to know more?
If you are interested in our DynaPro Plate Reader, DynaPro NanoStar or Möbiuζ™ instruments, please contact info@wyatt.com. Already a Customer? Please go to our support center [link] for tutorials, downloads of the latest software and more!
Operating system
Either 32-bit or 64-bit editions of Windows 7 or Windows Vista (including Business, Enterprise, or Ultimate
Editions), or Windows XP Professional 32-bit edition
System Requirements
Pentium IV or better processor with a minimum of 2 GHz clock speed.
1GB of RAM
An Ethernet port or USB 2.0 port for instrument communications
Other requirements:
CD or DVD drive for installation
100 MB of disk space for application and documentation
Wyatt Technology is the world leader in providing the most advanced macromolecular and nanoparticle characterization tools. These instruments and software may be used to determine the absolute molecular weight and or size of macromolecules and nanoparticles and include: multi-angle light scattering, dynamic light scattering, high through-put dynamic light scattering, field flow fractionation, refractive index, zeta potential and viscometry detection.
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