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Wyatt Technology provides the essential techniques for characterizing macromolecules and nanoparticles in solution, to determine molar mass, size, charge, and interactions.

Macromolecular and Nanoparticle Properties

Molar Mass

Multi-Angle static Light Scattering (MALS) measures molar mass directly, in solution. Combined with a fractionation technique like SEC or FFF, MALS determines absolute molar mass distributions - independent of elution time.

Size

The size of macromolecules or nanoparticles is measured via two light scattering methods: Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS). Each technique contributes critical pieces of the characterization puzzle.

Biomolecular Interactions

Biomolecular interactions are key to understanding a host of phenomena ranging from structure-function relationships and biotherapeutic activity through stability and aggregation.

Charge & Zeta Potential

Molecular charge and nanoparticle zeta potential are core properties of a solute and a solvent, relevant to colloidal stability, chemical modifications and other key qualities.

 

Techniques for Solution-Based Characterization

SEC-MALS

Conventional Size-Exclusion Chromatography (SEC) relies on reference standards that do not accurately represent your molecules. Multi-Angle Static Light Scattering (MALS) measures molar mass directly, independent of elution time, so you can have confidence in the values you report.

FFF-MALS

Field-Flow Fractionation (FFF) is a versatile separation technique that overcomes many of the limitations of analytical SEC. Combine with MALS and/or DLS for absolute molar mass and size.

CG-MALS

Composition-Gradient, Multi-Angle Static Light Scattering (CG-MALS) is a powerful technique for characterizing a wide range of biomolecular interactions, label-free and immobilization-free. CG-MALS is determines binding affinity, absolute stoichiometry, equilibrium and kinetics of simple or complex interactions.

DLS

Widely used in biochemistry, biotechnology and pharmaceutical development, Dynamic Light Scattering (DLS) is popular wherever the size and size distributions of macromolecules and nanoparticles need to be measured quickly and easily. Wyatt offers high-throughput, automated DLS as well as conventional formats.

MP-PALS

MP-PALS improves over conventional Phase Analysis Light Scattering to determine electrophoretic mobility, zeta potential and charge with maximum sensitivity and minimal damage to fragile biomolecules.