Wyatt Technology

Virtual Method Optimization and Size Analysis for FFF

Flow-field flow fractionation (flow-FFF) offers highly versatile separations for the analysis of complex fluids, covering a size range of macromolecules and particles from 1 nm to 10,000 nm. However, flow-FFF is often perceived as a difficult technique to learn because of the multiple parameters available for adjustment.

Our new SCOUT DPS® software simulates flow-FFF from basic physical principles, enabling the virtual optimization of flow-FFF methods and opening up the power of flow-FFF separations to non-experts. SCOUT DPS also analyzes particle size distributions (hydrodynamic radius) by elution time to increase characterization capabilities. This publication in The Column explains how it works. http://www.chromatographyonline.com/field-flow-fractionation-virtual-optimization-versatile-separation-methods-0.

FFF Illustration

Wyatt Customer to Win Nobel Prize in Chemistry

Wyatt Technology congratulates its customer, Dr. Joachim Frank of Columbia University on sharing the Nobel Prize in Chemistry today with Jacques Dubochet and Richard Henderson. We are delighted to see their work developing cryo-electron microscopy recognized for its use in high-resolution structure determination of biomolecules in solution. Some of Professor Frank’s publications using our DAWN, Optilab and NanoStar instruments may be found in our Bibliography. Here is a recently published paper in Science;
Y. Chen, O. B. Clarke, J. Kim, S. Stowe, Y.-K. Kim, Z. Assur, M. Cavalier, R. Godoy-Ruiz, D. C. von Alpen, C. Manzini, W. S. Blaner, J. Frank, L. Quadro, D. J. Weber, et al.. Structure of the STRA6 receptor for retinol uptake. Science 2016 353(6302).

This is just a sample of the many peer-reviewed articles utilizing Wyatt instruments that may be found by searching our online Bibliography.

Nobel Prize

Gold Seal of Quality Awarded for DAWN HELEOS

Just six months after receiving the SelectScience® Reviewers' Choice Award for 'Instrument of the Year', and four months after being awarded the Silver Seal of Quality, we are thrilled to announce that the rating of the DAWN HELEOS II on SelectScience has been upgraded to Gold Seal of Quality. SelectScience extends the Seal of Quality to less than 0.1% of the thousands of products reviewed on their site. At least 100 customer-provided reviews, plus an overall score of at least 4.5 stars out of 5, are required in order to obtain Gold status. Wyatt is very pleased to be one of the few to receive this recognition, and only the second analytical instrument featuring the Gold Seal of Quality.
We have already told the nail-biting story behind the decision to submit our products for review, and the happy outcome, at http://www.wyatt.com/blogs/silver-seal-of-quality-awarded-for-dawn-heleos.html. It does bear repeating that these reviews and awards are not 'bought-and-paid' self-promotion. SelectScience imposes strict rules regarding the reviews which are widely recognized as fair and objective. We are very grateful to all of our customers who submitted reviews: to those who submitted positive reviews, and to those whose reviews were less than positive, giving us important feedback on what needs to be improved. We look forward to more feedback – with your help, we hope to achieve Platinum!

gold seal

gold certificate

Scientific Reports: µSEC-MALS analysis of NIST mAbs

Increasingly UHPLC is being implemented throughout the biopharmaceutical industry, replacing HPLC for characterization of monoclonal antibodies and other therapeutic proteins with reduced consumption of sample and solvent, higher throughput and increased resolution. The µDAWN® multi-angle light scattering (MALS) detector was developed to meet the need for a low-volume, low-dispersion MALS instrument compatible with size-exclusion UHPLC (SE-UHPLC). µSEC-MALS combines the µDAWN with the Optilab® UT-rEX™ differential refractive index detector and a SE-UHPLC system for absolute molar mass and size measurements of proteins and other macromolecules.

“Measuring proteins with greater speed and resolution while reducing sample size”, authored by Drs. Vincent Hsieh and Philip Wyatt (both of Wyatt Technology) reports on the application of µSEC-MALS to NIST standard reference material SRM 8671, which was developed as the benchmark molecule against which many biomolecular analytical techniques are assessed and evaluated. Not only did µSEC-MALS produce data of comparable quality to standard SEC-MALS on monomer and aggregates with far less material and time investment, it was also able to discern and quantify a very small peak representing a fragment–that would appear to be a heavy chain of IgG with molar mass 44.9 kDa–not seen with standard SEC-MALS. An accelerated aggregation course highlighted the ability of the µDAWN to detect and quantify the weight-average molar masses of small high-molecular-weight (oligomer) peaks and resolve minimal amounts of dual-heavy-chain fragments, molar mass of 98.9 kDa, that ride on the monomer peak.

The article appears in Scientific Reports 7: 10030 and may be accessed freely at the nature.com website via DOI:10.1038/s41598-017-09051-1. Congratulations to Drs. Hsieh and Wyatt on this important publication!

MALS comparison

Three Wyatt Generations

3G isn’t just a telecommunications term anymore. It’s also an abbreviation for the three generations of Wyatts working at Wyatt Technology this summer. While there have been 2G—or two generations—of Wyatts working at Wyatt Technology for more than thirty years, this summer we welcomed Spenser Wyatt, Geof’s son, to intern under Ross Bryant, Wyatt Technology’s Vice President for Sales & Marketing, doing Market Research. Besides investigating instrument population density throughout the United States, and researching new sales tools, Spenser has audited Light Scattering University and surveyed students about their experiences at LSU. Spenser is a rising Junior at Emory University, in Atlanta, GA, and, like his father, is leaning towards a liberal arts degree. Meanwhile, Spenser’s grandfather, Phil, and uncle, Cliff, have been encouraging him to take some Physics and Chemistry classes! Cliff’s own son, Jake, is too young to work at the company yet, but has expressed in no uncertain terms his desire to run the company to fund his Lego acquisitions!

Three Wyatt Generations


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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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