Wyatt Technology

FDA and NIH investigate HT-DLS for nanoparticle stability

One of the major challenges in the development of nanoparticles (NPs) for drug delivery, phototherapy and diagnostics is assessing their stability under physiological conditions and, for photothermal therapies, under therapeutic conditions. The pH and ionic strength of standard blood and the intracellular matrix can negate the effects of charge-charge repulsion in such particles, lead to aggregation and even flocculation, hence preventing the penetration of the NPs into the targeted cells.

Another issue of concern is interaction of NPs with soluble blood components such as serum albumin and IgG. The attachment of these proteins to the NPs can be detrimental in terms of increased aggregation, but also via biochemical impact on delivery and/or reduction in efficacy of the NP payload. At the elevated temperatures typical of photothermal therapy, other types of degradation may occur such as dissociation of a polymer micelle.

In a therapeutic NP development effort, stability under physiological conditions and multiple temperature regimes must be evaluated for multiple candidate NPs, over an extended time scale. In our Featured Publication 36, Ashwinkumar Bhirde et al. of the FDA and NIH successfully demonstrate the use of Dynamic Light Scattering using the DynaPro DLS Plate Reader II to assess the colloidal stability of a several model NPs, their interaction with serum albumin, and the impact of the interaction on colloidal stability. The study greatly benefited from the full automation and high throughput provided by the DynaPro PR2. According to the authors, "this... high-throughput method can accomplish sophisticated hydrodynamic size measurement protocols within days instead of years it would take conventional hydrodynamic size measurement techniques to achieve a similar task"

nanoparticle protein interaction and stability
Illustration from Figure 1 of Bhirde et al 2017.
The study included measurements over a period of 3-5 days and temperature cycles between 37°C and 60°C, in both water and a medium containing bovine serum albumin (BSA). Au NP sizes ranged from 10-100 nm, and larger when PEGylated. One of the key results was the robustness of PEGylated Au NPs against the attachment of BSA, proving that high-throughput, plate-based DLS can readily distinguish between NPs coated and uncoated with protein as well as aggregation.

dynamic light scattering colloidal stability high throughput screening
Authors from the FDA and NIH have published on using the DynaPro Plate Reader II to study stability of a model colloidal system under physiological conditions
dynamic light scattering nanoparticle size and stability

Sigma XI Inducts 12 Wyatt Technology Scientists

Sigma XI

October 26, 2016

Wyatt Technology proudly announces that twelve of its scientists have been inducted into Sigma Xi, The Scientific Research Society. Sigma Xi is the world’s largest, multidisciplinary honor society for scientists and engineers. The Wyatt Scientists who have been inducted can be found here.

The newly inducted members join Sigma Xi’s global network of those who have been recognized for their achievements in research. “Wyatt Technology competes against some of the largest analytical instrument companies in the world. Our 30+ years of delighting our customers can be attributed to the scientific excellence of our Research and Development staff in bringing exceptional products to market. This announcement is a further validation of our organization’s continuing commitment to innovative research,” said Dr. Philip J. Wyatt, CEO of Wyatt Technology. 

Sigma Xi works to enhance the health of the research enterprise, foster integrity in science and engineering, and promote the public’s understanding of science. More information can be found at www.sigmaxi.org.

Wishing you the best of Holidays

Happy Holidays from Wyatt

December 22, 2016

On behalf of our entire company, I wanted to take this opportunity to wish you and your families a wonderful holiday season. The end of the year is, in its own way, a commencement of the New Year, and our company has enjoyed 34 years of prosperity thanks to your business. We never forget that you—our customers—are the entire reason we’re here! Without you, who knows what odd jobs we’d all be performing. We certainly wouldn’t be working with some of the most talented scientists in the world, that’s for sure! Our company is founded on the family-owned-and-operated premise that “this time, it’s personal.” We hope that this is reflected in all of our interactions with you, and that we’re not some nameless, faceless, analytical instrument company that could be replaced by some other entity.

Have a great holiday and please don’t ever hesitate to let us know how we can help you to be more productive with our instruments.

—Geofrey K. Wyatt

We’re moving! Just not very far...

Construction at 6330 Hollister
Construction at 6330 Hollister

Yes, it’s true. Wyatt Technology is moving its premises in January 2017. For those of you who have known—and loved—our location at 6300 Hollister Avenue, do not fret. We are moving about 100 meters away to 6330 Hollister Avenue. Not only will we still be located on the same street, but we’ll still be located in the same building. So why are we moving? It certainly isn’t because we like to move. Who likes to move?! We’re moving because we’ve simply run out of space. In the past 10 years our workforce here in Santa Barbara has more than tripled, but our space has only increased by a third. Now we’re moving to a space previously occupied by a medical robotics company that has about 4,200 square meters (45,000 square feet) of space—an increase of about 50% over what we currently have. We’ll have a much bigger and better applications lab, manufacturing space, service area, and our software and hardware teams will once again be working in the same general vicinity. We have new patio space being constructed, more offices and modular furniture, as well as better areas for all-company meetings and LSU lectures. So while we aren’t looking forward to the upheaval in January to get there, we are looking forward to getting settled!

Reviewer's Choice Awarded for DAWN HELEOS

At the recent Pittsburgh Conference (PittCon) held in Chicago, IL March 6-9, 2017, Wyatt Technology was pleasantly surprised to learn that we had won a Scientist’s Choice Award® from SelectScience. The active roster of 300,000+ SelectScience customers were invited to participate in the process by nominating, voting and reviewing companies of their choosing.

With such illustrious—and much better known companies (Agilent, Bruker, Thermo and Waters—to name but a few) in the mix—Wyatt Technology carried the trophy away for the Reviewer’s Choice Award™ for Instrument of the Year for the DAWN HELEOS MALS detector. At first we (Dr. Wyatt, Cliff and I) figured that this was probably going to turn out to be a “LaLa Land” type of Academy Awards goof, but when the applause died down no one was running around whispering that a mistake had been made! And no one from any of the afore-mentioned companies tried to take the trophy away from Dr. Wyatt. View his acceptance speech here

View Dr. Wyatt's acceptance speech here.

Read the editorial article of the Choice Awards here.

The award recognizes the significance of our customer service and support, as well as the engineering and technical know-how that goes into every DAWN HELEOS. We were thrilled to be acknowledged in such a public venue, and with such prestigious competition. But none of this would have been possible were it not for the enthusiastic voting of our loyal and delighted customers. Without them we’d all be working elsewhere. Their feedback, criticisms and accolades have all coalesced to make us the company we are today. We want to thank them for pushing us to be even better.

DAWN HELEOS Reviewer's Choice Award
Geofrey, Philip and Clifford Wyatt received the Reviewer's Choice award at PittCon 2017

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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