Wyatt Technology

Three Wyatt Generations

3G isn’t just a telecommunications term anymore. It’s also an abbreviation for the three generations of Wyatts working at Wyatt Technology this summer. While there have been 2G—or two generations—of Wyatts working at Wyatt Technology for more than thirty years, this summer we welcomed Spenser Wyatt, Geof’s son, to intern under Ross Bryant, Wyatt Technology’s Vice President for Sales & Marketing, doing Market Research. Besides investigating instrument population density throughout the United States, and researching new sales tools, Spenser has audited Light Scattering University and surveyed students about their experiences at LSU. Spenser is a rising Junior at Emory University, in Atlanta, GA, and, like his father, is leaning towards a liberal arts degree. Meanwhile, Spenser’s grandfather, Phil, and uncle, Cliff, have been encouraging him to take some Physics and Chemistry classes! Cliff’s own son, Jake, is too young to work at the company yet, but has expressed in no uncertain terms his desire to run the company to fund his Lego acquisitions!

Three Wyatt Generations

Featured Customer - Professor Christopher Ellison

It was our pleasure to host Prof. Christopher Ellison at the June LSU (Light Scattering University) course—held, as always, at Wyatt’s headquarters in beautiful Santa Barbara, California. As Piercy Professor of Chemical Engineering and Materials Science at the University of Minnesota, Dr. Ellison’s research pushes the boundaries of molecular architecture: he and his group create and investigate nanostructured polymeric systems for a wide array of advanced applications including photolithography, microelectronic device design, battery design, and general nano-scale manufacturing. Learn more about Dr. Ellison and his work by visiting https://ellison.dl.umn.edu/.

Dr. Ellison has found SEC-MALS-IV analysis of polymers to be valuable for his work in polymer engineering, providing comprehensive, absolute characterization of molar mass and intrinsic viscosity, including distributions and moments. The work reported in his 2014 Polymer paper, published while at his previous position with the University of Texas at Austin, made use of a complete triple-detection GPC system incorporating a DAWN HELEOS II, ViscoStar and Optilab T-rEX. Learn more about these applications at www.wyatt.com/polymers.

Now that Christopher has completed LSU training on SEC-MALS we look forward to more exciting research results utilizing the DAWN, and wish him much success advancing nanopatterning technology, as well as membranes, green manufacturing, flame retardants and all the other projects his group is pursuing.

Professor Christopher Ellison
Professor Christopher Ellison

FDA and NIH investigate HT-DLS for nanoparticle stability

One of the major challenges in the development of nanoparticles (NPs) for drug delivery, phototherapy and diagnostics is assessing their stability under physiological conditions and, for photothermal therapies, under therapeutic conditions. The pH and ionic strength of standard blood and the intracellular matrix can negate the effects of charge-charge repulsion in such particles, lead to aggregation and even flocculation, hence preventing the penetration of the NPs into the targeted cells.

Another issue of concern is interaction of NPs with soluble blood components such as serum albumin and IgG. The attachment of these proteins to the NPs can be detrimental in terms of increased aggregation, but also via biochemical impact on delivery and/or reduction in efficacy of the NP payload. At the elevated temperatures typical of photothermal therapy, other types of degradation may occur such as dissociation of a polymer micelle.

In a therapeutic NP development effort, stability under physiological conditions and multiple temperature regimes must be evaluated for multiple candidate NPs, over an extended time scale. In our Featured Publication 36, Ashwinkumar Bhirde et al. of the FDA and NIH successfully demonstrate the use of Dynamic Light Scattering using the DynaPro DLS Plate Reader II to assess the colloidal stability of a several model NPs, their interaction with serum albumin, and the impact of the interaction on colloidal stability. The study greatly benefited from the full automation and high throughput provided by the DynaPro PR2. According to the authors, "this... high-throughput method can accomplish sophisticated hydrodynamic size measurement protocols within days instead of years it would take conventional hydrodynamic size measurement techniques to achieve a similar task"

nanoparticle protein interaction and stability
Illustration from Figure 1 of Bhirde et al 2017.
The study included measurements over a period of 3-5 days and temperature cycles between 37°C and 60°C, in both water and a medium containing bovine serum albumin (BSA). Au NP sizes ranged from 10-100 nm, and larger when PEGylated. One of the key results was the robustness of PEGylated Au NPs against the attachment of BSA, proving that high-throughput, plate-based DLS can readily distinguish between NPs coated and uncoated with protein as well as aggregation.

dynamic light scattering colloidal stability high throughput screening
Authors from the FDA and NIH have published on using the DynaPro Plate Reader II to study stability of a model colloidal system under physiological conditions
dynamic light scattering nanoparticle size and stability

Sigma XI Inducts 12 Wyatt Technology Scientists

Sigma XI

October 26, 2016

Wyatt Technology proudly announces that twelve of its scientists have been inducted into Sigma Xi, The Scientific Research Society. Sigma Xi is the world’s largest, multidisciplinary honor society for scientists and engineers. The Wyatt Scientists who have been inducted can be found here.

The newly inducted members join Sigma Xi’s global network of those who have been recognized for their achievements in research. “Wyatt Technology competes against some of the largest analytical instrument companies in the world. Our 30+ years of delighting our customers can be attributed to the scientific excellence of our Research and Development staff in bringing exceptional products to market. This announcement is a further validation of our organization’s continuing commitment to innovative research,” said Dr. Philip J. Wyatt, CEO of Wyatt Technology. 

Sigma Xi works to enhance the health of the research enterprise, foster integrity in science and engineering, and promote the public’s understanding of science. More information can be found at www.sigmaxi.org.

Wishing you the best of Holidays

Happy Holidays from Wyatt

December 22, 2016

On behalf of our entire company, I wanted to take this opportunity to wish you and your families a wonderful holiday season. The end of the year is, in its own way, a commencement of the New Year, and our company has enjoyed 34 years of prosperity thanks to your business. We never forget that you—our customers—are the entire reason we’re here! Without you, who knows what odd jobs we’d all be performing. We certainly wouldn’t be working with some of the most talented scientists in the world, that’s for sure! Our company is founded on the family-owned-and-operated premise that “this time, it’s personal.” We hope that this is reflected in all of our interactions with you, and that we’re not some nameless, faceless, analytical instrument company that could be replaced by some other entity.

Have a great holiday and please don’t ever hesitate to let us know how we can help you to be more productive with our instruments.

—Geofrey K. Wyatt

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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