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Innovations and Applications of UHP-SEC and Multi-Angle Light Scattering (MALS) for the Analysis of Biotherapeutic Proteins

 

Presented Live: October 25, 2016

Speakers:
Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. John Champagne, Senior Applications Scientist, Wyatt Technology Corp.

Ultra-high performance size exclusion chromatography (UHP-SEC) is gaining in popularity owing to the high speed and resolution of separation compared to standard HP-SEC. Online multi-angle light scattering (MALS) is the recognized method for obtaining absolute molecular weight and size, independently of column retention time, making it indispensable for accurate and reliable characterization by SEC. However, light scattering detectors designed for HPLC-SEC are in general not suitable for UHP-SEC.

In this webinar we discuss the practical application of UHP-SEC with regards to method development and successful implementation. This will be followed by a discussion on limitations of standard MALS detectors and present the capabilities of a MALS detector designed explicitly to meet the challenges of UHP-SEC. The utility of UHP-SEC combined with MALS–dubbed ‘µSEC-MALS’–as well as online dynamic light scattering will be demonstrated in the context of protein characterization.

 

 

Small, but bright: µSEC-MALS adds light scattering to UHP-SEC for biophysical characterization of biotherapeutics

 

Speakers:
Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. Michelle Chen, Director of Analytical Services, Wyatt Technology Corp.
Mark Haverick, Senior Scientist, Merck & Co Inc.

UHP-SEC-MALS combines the high speed, high resolution, and low sample consumption of UHP-SEC separation with online multi-angle light scattering (MALS) analysis. MALS is the recognized, indispensable method for obtaining absolute molecular weight and size, independently of column retention time. In this webinar we present the key features of UHP-SEC as well as a MALS detector optimized to couple with it, creating a “µSEC-MALS” system for protein and synthetic polymer characterization.

Case studies will illustrate the applications of µSEC-MALS in the analysis and characterization of biopharmaceuticals. In particular we will discuss some of the challenges of bridging analytical-scale SEC-MALS to µSEC-MALS, as well as bridging between different UHP-SEC elution conditions.

 

 

Charge it! Electrophoretic Mobility as a Tool for Characterizing Nanoparticle Stability

 

Presented Live: June 2, 2015

Zeta potential is an indispensable measurement commonly used to guide efforts to control stability of particle suspensions. The recent surge of nanoparticle applications has challenged instruments that were designed for analyzing colloids and emulsions. This webinar presents new developments in instrumentation that have expanded the range and methods of zeta potential measurements.

Determination of zeta potential requires knowledge of both electrophoretic mobility and size. These are usually measured sequentially and can potentially damage fragile biomolecules due to high applied voltage. The advances in phase analysis light scattering, described in the webinar, enable highly sensitive measurements to be conducted rapidly at low voltage. They also provide real-time monitoring of samples by simultaneous dynamic light scattering to detect potential degradation, in addition to providing the requisite size data. In addition, sample collection can now be fully automated with an auto-sampler to allow unattended operation. Applications presented will include characterization of drug conjugates, vaccine adjuvants, proteins, and gold nanoparticles.

 

 

 

Essential Polymer Characterization with MALS and FFF

 

Presented Live: January 12, 2016

Characterization techniques based on multi-angle light scattering with chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) address many of the key analytical challenges in the modern polymer laboratory. This seminar will review light scattering technology, instrumentation and applications to polymer research. Select examples will illustrate how a complete light scattering solution based on SEC-MALS facilitates rapid and effective characterization of a wide variety of polymers, including polyacrylamides, acrylic copolymers, nylons, styrenes and more.

Recent advances in commercial applications of high molecular weight and/or branched polymers pose a particular challenge to analytical systems that were designed for simpler polymers. Case studies will be presented to illustrate failures of single-detector chromatography or SEC-MALS, wherein the addition of MALS to FFF made proper analysis possible. The supplemental roles of Dynamic Light Scattering (DLS) and Viscometry will also be explored.

 

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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