Wyatt Technology

What Can I Analyze?



    While SEC-MALS with a DAWN or miniDAWN is the primary means of characterizing soluble protein aggregates, FFF-MALS, CG-MALS and DLS are valuable orthogonal techniques to meet FDA requirements.



    SEC or FFF plus triple detection UV-MALS-RI and ASTRA software determine the molar mass of protein and modifier in conjugated biotherapeutics such as antibody-drug conjugates or PEGylated proteins.



    The DynaPro Plate Reader II performs high-throughput screening of excipients and buffers for optimal formulation in situ in standard microwell plates, detecting aggregation as well as conformational and colloidal stability indicators.



    CG-MALS combines a Calypso II and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of drug-target binding, without labeling or immobilization. It also analyzes self-association at low, moderate and high concentrations.


  • Identification



    A DAWN or miniDAWN MALS detector, combined with a Wyatt SEC column and the Optilab T-rEX dRI detector, determine absolute molar masses and sizes of peptides, proteins and other biomacromolecules independently of column calibration.




    Validate protein purification and stability quickly and easily with a DynaPro NanoStar cuvette-based, combined dynamic light scattering & static light scattering detector, or a high-throughput DynaPro Plate Reader II.




    The Möbiuζ is the only electrophoretic light scattering detector capable of providing reliable and repeatable measurements of a protein's net charge in high-salt buffers.




    CG-MALS combines a Calypso and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of simple or complex biomolecular interactions. CG-MALS requires no labeling or immobilization, addressing self-association as well as hetero-association.


  • Absolute Molar Mass & Size DAWN HELEOS II Optilab T-rEX


    Absolute Molar Mass and Size

    A DAWN MALS detector and Optilab T-rEX dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration.




    Light scattering is an excellent means for assessing the degradation of biopolymers as a result of exposure to heat, light, high or low pH, and other stimuli.




    Key ingredients in cosmetics, the paper industry and food products, polysaccharides are also critical materials in biomedical applications such as vaccines and blood coagulants. Characterize all types of polysaccharides by SEC combined with Wyatt's on-line light scattering, dRI and viscosity detectors.

    Paper & Pulp


    Paper and Pulp

    Lignin and cellulose present some of the most challenging biopolymeric analytes due to their unique optical and morphological properties. Wyatt offers multiple solutions for meeting these challenges.


  • Absolute Molar Mass & Size DAWN HELEOS II Optilab T-rEX


    Absolute Molar Mass and Size

    A DAWN MALS detector and Optilab T-rEX dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration.

    Branching and Conformation


    Branching and Conformation

    Incorporate a WyattQELS DLS module and/or a ViscoStar II differential viscometer to characterize the branching ratio and conformation of complex polymers.

    FFF, Field-Flow Fractionation


    Field-Flow Fractionation

    The Eclipse DualTec provides true hydrodynamic fractionation without the stress, surface interactions and non-ideal separation present in SEC. Integrates perfectly with Wyatt MALS, dRI, DLS and viscometry detectors.

    dn/dc and zimm plot


    dn/dc and
    Zimm plots

    Wyatt offers several options to simplify laborious, error-prone dn/dc and Zimm analyses for natural and synthetic polymers.


  • FFF, field flow fractionation


    Accurate Size Distributions

    Accurate size distributions with true ensemble representation require fractionation + independent, on-line determination of size. A DAWN HELEOS II / Eclipse FFF-MALS system can characterize simple or complex fluids incorporating macromolecules with large and small nanoparticles.

    Conformation & Composition


    Conformation and Composition

    Information about nanoparticle shape and structure can be obtained by combining MALS analysis with dynamic light scattering. The WyattQELS DLS module integrates into the DAWN or miniDAWN for simultaneous MALS and DLS analysis. Add a UV, fluorescence and/or Optilab T-rEX dRI detector for compositional studies.



    High-Throughput Sizing

    Need to characterize your nanoparticles under hundreds of solvent conditions in the space of a few hours? The DynaPro Plate Reader II measures size distributions by DLS, in situ in standard 96-, 384- or 1536-well microtiter plates. A camera conveniently photographs each well to identify precipitates.

    interaction zeta potential


    Zeta Potential / Charge

    The Möbiuζ is the only zeta potential detector capable of hands-free, high-throughput measurements by coupling to an autosampler. It measures charge and size simultaneously to monitor aggregation or degradation caused by the applied electric field.


  • Home

Innovations and Applications of UHP-SEC and Multi-Angle Light Scattering (MALS) for the Analysis of Biotherapeutic Proteins


Presented Live: October 25, 2016

Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. John Champagne, Senior Applications Scientist, Wyatt Technology Corp.

Ultra-high performance size exclusion chromatography (UHP-SEC) is gaining in popularity owing to the high speed and resolution of separation compared to standard HP-SEC. Online multi-angle light scattering (MALS) is the recognized method for obtaining absolute molecular weight and size, independently of column retention time, making it indispensable for accurate and reliable characterization by SEC. However, light scattering detectors designed for HPLC-SEC are in general not suitable for UHP-SEC.

In this webinar we discuss the practical application of UHP-SEC with regards to method development and successful implementation. This will be followed by a discussion on limitations of standard MALS detectors and present the capabilities of a MALS detector designed explicitly to meet the challenges of UHP-SEC. The utility of UHP-SEC combined with MALS–dubbed ‘µSEC-MALS’–as well as online dynamic light scattering will be demonstrated in the context of protein characterization.



Small, but bright: µSEC-MALS adds light scattering to UHP-SEC for biophysical characterization of biotherapeutics


Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. Michelle Chen, Director of Analytical Services, Wyatt Technology Corp.
Mark Haverick, Senior Scientist, Merck & Co Inc.

UHP-SEC-MALS combines the high speed, high resolution, and low sample consumption of UHP-SEC separation with online multi-angle light scattering (MALS) analysis. MALS is the recognized, indispensable method for obtaining absolute molecular weight and size, independently of column retention time. In this webinar we present the key features of UHP-SEC as well as a MALS detector optimized to couple with it, creating a “µSEC-MALS” system for protein and synthetic polymer characterization.

Case studies will illustrate the applications of µSEC-MALS in the analysis and characterization of biopharmaceuticals. In particular we will discuss some of the challenges of bridging analytical-scale SEC-MALS to µSEC-MALS, as well as bridging between different UHP-SEC elution conditions.



Charge it! Electrophoretic Mobility as a Tool for Characterizing Nanoparticle Stability


Presented Live: June 2, 2015

Zeta potential is an indispensable measurement commonly used to guide efforts to control stability of particle suspensions. The recent surge of nanoparticle applications has challenged instruments that were designed for analyzing colloids and emulsions. This webinar presents new developments in instrumentation that have expanded the range and methods of zeta potential measurements.

Determination of zeta potential requires knowledge of both electrophoretic mobility and size. These are usually measured sequentially and can potentially damage fragile biomolecules due to high applied voltage. The advances in phase analysis light scattering, described in the webinar, enable highly sensitive measurements to be conducted rapidly at low voltage. They also provide real-time monitoring of samples by simultaneous dynamic light scattering to detect potential degradation, in addition to providing the requisite size data. In addition, sample collection can now be fully automated with an auto-sampler to allow unattended operation. Applications presented will include characterization of drug conjugates, vaccine adjuvants, proteins, and gold nanoparticles.




Essential Polymer Characterization with MALS and FFF


Presented Live: January 12, 2016

Characterization techniques based on multi-angle light scattering with chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) address many of the key analytical challenges in the modern polymer laboratory. This seminar will review light scattering technology, instrumentation and applications to polymer research. Select examples will illustrate how a complete light scattering solution based on SEC-MALS facilitates rapid and effective characterization of a wide variety of polymers, including polyacrylamides, acrylic copolymers, nylons, styrenes and more.

Recent advances in commercial applications of high molecular weight and/or branched polymers pose a particular challenge to analytical systems that were designed for simpler polymers. Case studies will be presented to illustrate failures of single-detector chromatography or SEC-MALS, wherein the addition of MALS to FFF made proper analysis possible. The supplemental roles of Dynamic Light Scattering (DLS) and Viscometry will also be explored.





For essential macromolecular and nanoparticle characterization—The Solution is Light™

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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