Polymer characterization: viscosity is intrinsic
December 15, 2017 - The centrality of multi-angle light scattering to polymer characterization is widely appreciated. SEC-MALS with a DAWN® HELEOS II is the gold standard for determining reliable, accurate distributions of absolute molar mass and rms radius, without having to rely on column calibration, reference molecules that don’t elute like your samples, and assumptions about ideal conformation and column interactions. But once you have mastered SEC-MALS, what’s the next stage? How to obtain even deeper understanding of your macromolecules? And what are your options if SEC-MALS does not work for some of your samples?
Featured Customer: Charles Diesendruck
December 14, 2017 - Serial customer’ is not quite as chilling a phrase as ‘serial killer’, but still it can make us a bit nervous. We know that a serial customer’s research may be so dependent on Wyatt products that they keep coming back, again and again, to fulfill their need for yet another fresh SEC-MALS stack. A career may depend on Wyatt detectors; we don’t take that responsibility lightly! Charles Diesendruck, our Featured Customer for December 2017, is one such serial customer.
Featured Customer: Professor John Matson
November 27, 2017 - Prof. Matson’s research focuses on the synthesis of unique polymeric and supramolecular materials with applications in signaling gas delivery, tissue engineering, and sustainability. Current areas of interest include the development of materials for delivery of hydrogen sulfide (H2S), a vital biological signaling gas, the synthesis of cone-shaped bottlebrush polymers, and the preparation of new polymers and polymer blends derived from renewable feedstocks.
Developing a Library of Proteins for Drug Research
November 14, 2017 - The abundance of protein drugs on the market shows there is an increasing demand for knowledge within the formulation of biologics, as Professor Pernille Harris, Principal Investigator and Coordinator of the Protein-excipient Interactions and Protein-Protein Interactions in formulation (PIPPI) project explains
Featured Customer: Jon Pokorski
October 13, 2017 - Nanoparticle sizing, protein buffer optimization for crystallization, biotherapeutic stability evaluation, critical aggregation or micelle concentration. These are some of the more common applications and uses of the DynaPro® Plate Reader, including the just-launched Plate Reader III. But, some of our Plate Reader customers are far more creative than the average scientist.
DAWN Takes the Gold
October 13, 2017 - Once again our remarkable customers, along with SelectScience®, have chosen to recognize the DAWN® HELEOS® II as an icon of instrument quality and customer delight. This month the DAWN was awarded the SelectScience Gold Seal of Quality, just the second analytical instrument in a field of thousands to receive this status.
Wyatt Customer to Win Nobel Prize in Chemistry
October 4, 2017 - Wyatt Technology congratulates its customer, Dr. Joachim Frank of Columbia University on sharing the Nobel Prize in Chemistry today with Jacques Dubochet and Richard Henderson. We are delighted to see their work developing cryo-electron microscopy recognized for its use in high-resolution structure determination of biomolecules in solution.
Featured Customer: Professor Stuart Rowan
September 19, 2017 - We are pleased to announce that Prof. Stuart Rowan is our Featured Customer for September 2017. As Professor of Molecular Engineering at the University of Chicago’s Institute for Molecular Engineering, Dr. Rowan’s research focuses on the construction and properties of structurally-dynamic polymeric materials such as self-healing materials. He and his group study metallosupramolecular and stimuli-responsive polymers, cellulose nanocrystals in biomimetic and porous systems, and reversible covalent chemistry.
Virtual Method Optimization and Size Analysis for FFF
September 11, 2017 - Flow-field flow fractionation (flow-FFF) offers highly versatile separations for the analysis of complex fluids, covering a size range of macromolecules and particles from 1 nm to 10,000 nm. However, flow-FFF is often perceived as a difficult technique to learn because of the multiple parameters available for adjustment.
Our new SCOUT DPS® software simulates flow-FFF from basic physical principles, enabling the virtual optimization of flow-FFF methods and opening up the power of flow-FFF separations to non-experts. SCOUT DPS also analyzes particle size distributions (hydrodynamic radius) by elution time to increase characterization capabilities. This publication in The Column explains how it works.
Scientific Reports: µSEC-MALS™ analysis of NIST mAbs
August 31, 2017 - The µDAWN® multi-angle light scattering (MALS) detector was developed to meet the need for a low-volume, low-dispersion MALS instrument compatible with size-exclusion UHPLC (SE-UHPLC). Congratulations to Drs. Vincent Hsieh and Philip J. Wyatt on the publication of their article, “Measuring proteins with greater speed and resolution while reducing sample size” in Scientific Reports. The paper describes the analysis of NIST standard reference material SRM 8671, a monoclonal antibody developed to benchmark analytical instruments for the biopharmaceutical industry, with the µDAWN.
Three Wyatt Generations
August 11, 2017 - 3G isn’t just a telecommunications term anymore. It’s also an abbreviation for the three generations of Wyatts working at Wyatt Technology this summer. While there have been 2G—or two generations—of Wyatts working at Wyatt Technology for more than thirty years, this summer we welcomed Spenser Wyatt, Geof’s son, to intern under Ross Bryant, Wyatt Technology’s Vice President for Sales & Marketing, doing Market Research. Besides investigating instrument population density throughout the United States, and researching new sales tools, Spenser has audited Light Scattering University and surveyed students about their experiences at LSU.
Featured Customer: Professor Christopher Ellison
August 10, 2017 - It was our pleasure to host Prof. Christopher Ellison at the June LSU (Light Scattering University) course–held, as always, at Wyatt’s headquarters in beautiful Santa Barbara, California. As Piercy Professor of Chemical Engineering and Materials Science at the University of Minnesota, Dr. Ellison’s research pushes the boundaries of molecular architecture: he and his group create and investigate nanostructured polymeric systems for a wide array of advanced applications including photolithography, microelectronic device design, battery design, and general nano-scale manufacturing.
Drug discovery: Finding, then losing, then finding again colloidal aggregates of small-molecule drugs with DLS
July 1, 2017 - Dynamic light scattering is not just for proteins and nanoparticles. In the field of small-molecule drug discovery, high-throughput DLS with the DynaPro Plate Reader II has at least one key role - preventing the accumulation of misleading hits, or false positives, that are a result of aggregated compounds. Brian Shoichet's UCSF lab has been instrumental in elucidating the mechanism of action and prevention of these promiscuous inhibitors, utilizing the DynaPro PRII for detection and formulation. Read highlights of his publications and a link to a recent online interview.
Joining Forces for Faster Analyses
May 31, 2017 - When two technology leaders team up to advance the state of the art in polymer and biopharmaceutical characterization, customers can expect to see some real benefits coming down the pipeline. Wyatt and Waters have been collaborating for some time on joint educational efforts around UPLC®, APC and the μDAWN®, but now it is official. In the words of Cliff Wyatt, Executive Vice President of Wyatt Technology, “We’re delighted to be partnering these ‘best in class’ products to improve the accuracy and throughput of our mutual customers.” Click here to read the press release on our co-marketing agreement with Waters.
The Next Generation Compact MALS Detector
May 19, 2017 - Introducing the next generation miniDAWN: the miniDAWN® TREOS® II. In designing the TREOS II, your productivity was our guiding light. The TREOS II is modular and field-serviceable, adds ease-of-use features to make SEC-MALS even easier, and is upgradeable to a μDAWN. This new instrument is also more sensitive than the previous TREOS and has a larger range of size (hydrodynamic radius) measurement. To download a white paper describing the many improvements introduced in the TREOS II, click here.
Protein Quality Control in SPR and BLI High-Throughput Screening Studies
May 9, 2017 - Protein-protein binding studies are only as good as the quality of the reagents used in the experiments. Great resources in terms of instrumentation, sample production and scientist time are typically invested in high-throughput screening of antibodies or fragments to obtain optimal therapeutic candidates, perform epitope binning and rank candidates by affinity and kinetics. However, often little attention is paid to verifying protein solution quality—which can lead researchers down the proverbial rabbit hole, chasing after ephemeral or anomalous results that are caused by degraded proteins. To download a white paper describing a fast, easy and non-perturbing pre-screening method using the DynaPro Plate Reader II, click here.
Wyatt presents Polymer Characterization seminar hosted by the University of Minnesota
April 13, 2017 – Wyatt Technology, in partnership with the University of Minnesota, presented a polymer characterization seminar in the Twin Cities to over fifty scientists and engineers. Dr. Jeff Ahlgren was the keynote speaker and discussed ways in which size-exclusion chromatography (SEC) or gel permeation chromatography (GPC) coupled with multi-angle light scattering (MALS), refractive index (RI) and differential viscometry can be a powerful technique in determining important polymer characteristics such as: absolute molar mass, size, branching and conformation. Many of our long time and loyal customers attended and got a chance to hear about the latest developments at Wyatt Technology including the new, benchmark-setting ViscoStar III. Read more...
Wyatt staff gathers for ribbon-cutting ceremony at new facility
April 4, 2017 - Most of the boxes have been unpacked. Furniture that didn’t survive the move has been recycled. New logos have been stenciled on the glass. Our company sign has been hoisted into place on the building’s façade. Read more...
Reviewer's Choice Awarded for DAWN HELEOS II
March 14, 2017 - At the recent Pittsburgh Conference (PittCon) held in Chicago, IL March 6-9, 2017, Wyatt Technology was pleasantly surprised to learn that we had won a Scientist’s Choice Award® from SelectScience. The active roster of 300,000+ SelectScience customers were invited to participate in the process by nominating, voting and reviewing companies of their choosing. Read more...
DynaPro High-Throughput Dynamic Light Scattering Now Available with 21CFR11 Compliance
March 3, 2017 - We are pleased to announce the release of 21CFR(11)-compliant software for the groundbreaking DynaPro® Plate Reader II. The DynaPro PRII is widely used in the biopharmaceutical industry to characterize aggregation as well as thermal, conformational and colloidal stability of biotherapeutics. With the release of the 21CFR11-compliant Security Pack (SP) in DYNAMICS, labs operating under GLP or GMP regulatory requirements may now take advantage of the DynaPro PRII’s automation to measure dozens, hundreds or even thousands of samples per day. DYNAMICS SP also supports the DynaPro NanoStar cuvette-based DLS detector and the Möbius zeta potential/DLS detector.
New Application Note: Assessing Ligand-Binding Assay Reagent Proteins by SEC-MALS
March 3, 2017 - Ligand Binding Assays (LBAs) used for large molecule drug development often utilize proteins conjugated to various tags. To ensure that these conjugated protein reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically. This application note, contributed by Justin Low et al. at Genentech, discusses the advantages of SEC-MALS for this application and the impact of aggregated reagents on assays used to detect anti-drug antibodies. Download here.
Webcast: High Throughput Tools for Optimizing Drug Nanosuspensions
February 2, 2017 - Listen to this on-demand webcast to learn how Dr. Dennis Leung of Merck & Co. (currently at Genentech) has implemented high-throughput process development tools for drug nanosuspensions, utilizing high-throughput dynamic light scattering for rapid analytical feedback. View the webcast
Featured article: Algorithm-driven high-throughput screening of colloidal nanoparticles under simulated physiological and therapeutic conditions
February 14, 2017 - Testing the stability of nanoparticles for drug delivery, phototherapy and diagnostics under physiological conditions is a critical phase of development. Bhirde et al. of the FDA and NIH have demonstrated the use of the DynaPro DLS Plate Reader for assessing the stability of model colloidal systems and their interaction with serum albumin, fully automated over many samples conditions and an extended time period. Read more...
Webcast: The Molecular Tool-kit of Hemorrhagic Fever Viruses
February 2, 2017 - The ability of viruses to maximize functionality from a limited genome, and to evolve their functionalities in real time offers us fundamental insights into the capabilities and plasticity of proteins in general. In this on-demand webcast, Dr. Erica Ollmann Saphire of the Scripps Research Institute explores the versatile structural biology of viral proteins and how light scattering played a key role in her discoveries. View the webcast.
Screening Developability and Pre-Formulation of Biotherapeutics with High-Throughput Dynamic Light Scattering (HT–DLS)
February 2, 2017 - Learn how high-throughput dynamic light scattering meets the challenges of aggregation and stability studies in early developability and pre-formulation studies of biotherapeutics. Newly released white paper. Download here.
ViscoStar III - Innovations in Online Viscometry for GPC
January 21, 2017 - The ViscoStar III comprises breakthrough technology for online viscometry, enhancing sensitivity and resolution while improving ease-of-use and nearly eliminating sensitivity to pressure fluctuations from the HPLC pump or other sources. This white paper describes the innovations and the end benefits to users. Download here.
We’re moving! Just not very far...
January 9, 2017 - Yes, it’s true. Wyatt Technology is moving its premises in January 2017. For those of you who have known—and loved—our location at 6300 Hollister Avenue, do not fret. We are moving about 100 meters away to 6330 Hollister Avenue. Read more...
Wishing you the best of Holidays
December 22, 2016 - On behalf of our entire company, I wanted to take this opportunity to wish you and your families a wonderful holiday season. The end of the year is, in its own way, a commencement of the New Year, and our company has enjoyed 34 years of prosperity thanks to your business. Read more...
Commercializing multi-angle light scattering instruments - an interview with Philip Wyatt
November 11, 2016 - The fascinating story behind the origins of MALS instrumentation that revolutionized absolute molar mass and size analysis of proteins, polymers and nanoparticles. Read more...
Sigma Xi inducts 12 Wyatt Technology scientists
October 26, 2016 - Sigma Xi Inducts 12 Wyatt Technology Scientists October 26, 2016-Wyatt Technology proudly announces that eleven of its scientists have been inducted into Sigma Xi, The Scientific Research Society. Sigma Xi is the world’s largest, multidisciplinary honor society for scientists and engineers. Read more...
Cutting edge women use cutting edge instruments
October 18, 2016 - If you were watching the Today Show on Monday, October 17, 2016 you would have seen at least one segment not related to politics. In fact, “Cutting Edge Women”, is a regular part of the Today Show. As you might guess, it’s about influential women and this episode featured Anne Wojcicki, the founder and CEO of 23andMe, a genetic testing company in the Bay Area of California. 23andMe is also of greater interest to Wyatt Technology because Anne Wojciki’s company is a customer of our technology. Read more...
Chemistry Nobel Prize awarded to Sir J. Fraser Stoddart
October 5, 2016 - Wyatt Technology congratulates its customer, Sir J. Fraser Stoddart of Northwestern University on sharing the Nobel Prize in Chemistry today with Bernard L. Feringa and Jean-Pierre Sauvage. They have developed molecules with controllable movements, which can perform a task when energy is added. We are delighted to see their work on nano-motors recognized as the building blocks for future molecular machines. Some of Professor Stoddart’s publications using our DAWN instruments may be found in our Bibliography. Read more...
Highlights: Light scattering reveals novel applications of DNA
March 29, 2016 - Deoxyribonucleic acid (DNA) is an amazingly versatile molecule thanks to its unique structure and stability. Encoding and passing on to subsequent generations all inheritable genetic information, the DNA molecule plays a well-known, pivotal role in the biological world. However, DNA has also been the subject of cutting-edge research in fields outside the usual scope of genetics and biology, from nanotechnology and nanoelectronics to drug delivery and even optogenetics, taking advantage of its highly customizable properties. Light scattering, of course, plays an important part in many of these studies. Read more...
The myriad applications of quantitative light scattering in the biological and biopharmaceutical sciences
March 28, 2016 - Actually too numerous to cover in a single publication, but Allen Minton of the Laboratory of Biochemistry and Genetics in NIH’s National Institutes of Diabetes and Digestive and Kidney Diseases has done a spectacular job of critically addressing quite a few. His review article, “Recent applications of light scattering measurement in the biological and biopharmaceutical sciences,” appears in Analytical Biochemistry 501 (2016) pp. 4-22. Read more...
Retooling protein characterization
February 15, 2016 - Dr. Lisa Heiden of Genetic Engineering & Biotechnology News states if the Biotherapeutics Workshop is to progress beyond Rough-Hewn Products, it will need a range of finely calibrated analytical Platforms. DLS (dynamic light scattering) assesses size distribution, and MALS (multiangle light scattering) measures molar mass. Both are utilized throughout drug discovery, beginning with quality assessments of proteins to identify the best candidate molecules. Read more...
ZoBio optimizes solution behavior of proteins
February 10, 2016 - ZoBio Staff In many projects, the largest hurdle to be overcome is preparation of a protein that is well-behaved for biophysical and structural biological studies. Often even after achieving high level expression of a soluble, functional variant of the target, it may aggregate under the high concentrations required for many experimental techniques, particularly NMR and X-ray crystallography. Read more...
Wyatt’s International Light Scattering Colloquium focuses on the nano world
January 27, 2016 - Dr. Robert L. Stevenson presents highlights from the 24th ILSC in American Laboratory. Featured talks include those by Erica Ollmann Saphire of Scripps Research Institute, Frank von der Kammer of the University of Vienna, Joseph Arndt of biogen, David Cistola of University of North Texas Health Science Center and Joseph Glajch of Momenta Pharmeceuticals. Read more...
Light scattering in the nano world
January 20, 2016 - Methods for the analysis of nanoparticles have been available since the 1980s and light scattering offers one of the most versatile sets of characterization tools. The three most common variations of analytical light scattering includes: multi-angle, dynamic and electrophoretic (MALS, DLS and ELS, respectively). The light scattering toolbox comprising MALS, DLS and ELS is an essential kit for scientists and engineers working in nanoparticle characterization. Read more...
Protein formulation consortium
December 16, 2015 - Scientists in the fields of structural biology, biophysics, protein formulation and stability have formed a consortium, Protein-excipient Interactions and Protein-Protein Interactions in Formulation (PIPPI). PIPPI’s goals are a mechanistic understanding of protein stability, as well as training a new generation of innovative and entrepreneurial early-stage researchers who will develop methodologies and tools to guide the formulation of robust biologics. Three-year scholarships are available for 15 Ph.D. students. Read more...
Recent publications in the field of natural polymers
December 15, 2015 - Natural polymers and their derivatives feature in a host of cutting-edge applied research projects, including biofuels, environmentally friendly structural materials, nanopharmaceuticals and food science. Light scattering is, of course, a primary means for characterizing natural polymers in terms of molar mass, size, and conformation. Here we present some highlights of recent publications utilizing MALS to study polymerization, structure and degradation of natural polymers, and even the contribution of these materials to the texture and consistency of one of our favorite mobile phases: beer. Read more...
The chromatography detector you shouldn't miss
Nov. 20, 2015 - The question of 'Which chromatography detector should I buy' was addressed in a recent LinkedIn post, which gave a great overview of the various detectors available for many types of chromatography, but only briefly alluded to the most important detector type for anyone running size-exclusion chromatography (SEC) or gel-permeation chromatography (GPC): multi-angle light scattering (MALS). Read more...
Wyatt Technology’s growth continues…
Nov. 18, 2015 - Wyatt Technology Europe GmbH, Wyatt Technology's subsidiary office in Dernbach, Germany, has expanded its headquarters. The grand opening ceremony of the new European center of operations was held in conjunction with the European Users' Meeting, September 29th through October 2nd, 2015. Read more...
ILSC 2015: Scattered Light
November 15, 2015 - Wyatt Technology hosted the 24th International Light Scattering Colloquium bringing together dozens of scientists to discuss the use of light scattering in diverse applications from polymer science and nanoparticles to viruses and therapeutic proteins. Read more...