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Virtual Method Optimization and Size Analysis for FFF

Flow-field flow fractionation (flow-FFF) offers highly versatile separations for the analysis of complex fluids, covering a size range of macromolecules and particles from 1 nm to 10,000 nm. However, flow-FFF is often perceived as a difficult technique to learn because of the multiple parameters available for adjustment.

Our new SCOUT DPS® software simulates flow-FFF from basic physical principles, enabling the virtual optimization of flow-FFF methods and opening up the power of flow-FFF separations to non-experts. SCOUT DPS also analyzes particle size distributions (hydrodynamic radius) by elution time to increase characterization capabilities. This publication in The Column explains how it works. http://www.chromatographyonline.com/field-flow-fractionation-virtual-optimization-versatile-separation-methods-0.

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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