A SEC-MALS method is developed to quantify a number of biophysical attributes of mRNA, including molecular weight, percentage of aggregates, R_g, R_h, and R_g/R_h.

This application note shows the capabilities of asymmetric-flow field-flow fractionation (AF4) in combination with differential refractive index measurement plus static and dynamic light scattering (AF4-MALS-DLS) for a gentle separation and investigation of caseins enzymatically cross-linked with mTGase. Using this approach we have gained essential knowledge on the modus operandi of mTGase on size and conformation of caseins.

Size-exclusion chromatography with multi-angle light scattering (SEC-MALS), alone or in conjunction with a viscometer (SEC-MALS-IV) is often used to characterize LCB in macromolecules. A simple means of distinguishing between LCB and SCB would be of great value to polymer scientists. This application note describes how SEC-MALS identifies and quantifies these two properties.

Ligonsulfonates require costly, time-consuming purification of salts, sugars and other low-molecular-weight contaminants prior to analysis by GPC, in order to prevent column damage. This application note describes the use of AF4-MALS to characterize lignosulfates.

This application note describes a plate-based method for early process development of a compound in nanoparticle format that is rapid and requires very little material, combining acoustic resonance milling and automated DLS. These qualities make it appropriate for implementation in the discovery phase where a nimble strategy is imperative.

The WyattQELS dynamic light scattering module can be embedded in a DAWN multi-angle light scattering instrument for the simultaneous determination of molar mass and hydrodynamic radius. Here measurements of a globular protein, BSA, are compared with those of a glycoprotein.

Ligand Binding Assays (LBAs) used for large molecule drug development can necessitate conjugation of a drug to one or more different tags. To help ensure that LBAs utilizing conjugated drug reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically prior to being incorporated into the LBA.

Asymmetric Flow Field-Flow Fractionation (AF4) separated and characterized both the (large) guest alone, and—on account of the high concentrations achieved at the membrane—the host-guest complex. The millimolar concentration obtained during the separation step prevented dissociation of the conjugate, permitting determination of the complex stoichiometry and its stability in solution.

This application note describes measurements made using a stopped-flow MALS-SAXS set-up that was implemented at the SWING beamline of the SOLEIL Synchrotron near Paris, France. The apparatus was developed for the study of the formation of fibrin networks following enzymatic activation of fibrinogen molecules.

In this application note, we perform rapid UHP-SEC-MALS analysis on two antibodies under various conditions mimicking different manufacturing and purification processing steps.

Defining a composition profile is therefore an essential aspect of copolymer product development. Learn more about copolymer analysis by reading our application note.

The goal of this investigation is to accurately evaluate the TiO2 nanoparticle dispersion in different cell culture media using the high-throughput dynamic light scattering DynaPro® Plate Reader. To improve the dispersion of these nanoparticles, dispersing agents such as bovine serum albumin (BSA) and fetal bovine serum (FBS) were explored.