August 7, 2019

AN1603: Protein Reagent Quality for Drug Discovery

Quality and consistency in reagents is critical to successful drug discovery and development. When targeting a particular protein of interest, in vitro experiments should be performed with proteins of biological properties similar to those for in vivo tests. It is important that molecularity, purity, shape and degree of heterogeneity remain the same when any alterations are made.

August 7, 2019

AN8002: Reverse-Phase/MALS Characterization of Protein Oligomers – Fibroblast Growth Factor

Reversed-phase chromatography (RPC) represents one of the most popular applications of HPLC, with particular importance for protein characterization. Adding a DAWN or miniDAWN multi-angle light scattering (MALS) detector to one’s HPLC system, however, simplifies protein identification significantly. It allows one to measure absolute molar masses directly, irrespective of retention time, and to observe the properties of the protein in solution.

August 7, 2019

AN1613: Low-Molar-Mass Peptide Characterization by SEC-MALS

SEC-MALS is not just for large proteins - it can even be used for small peptides below 1 kDa. In fact, a DAWN® or miniDAWN® multi-angle light scattering (MALS) detector is instrumental in obtaining on-line molar mass determinations for peptides of fewer than a thousand Daltons. Learn more by reading our application note.

August 7, 2019

AN1602: Lipid/membrane protein complexes analyzed by SEC-MALS

In this application note we demonstrate the use of multi-angle light scattering (MALS) detection in combination with UV absorption and differential refractive index (DRI) detection to determine the molar masses (MM) of both the core protein and the entire protein-lipid complex.