Upcoming Live Webinars

Measuring AAV Quality Attributes with Light Scattering

Join us for a webinar on Tuesday, October 19 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

Adeno-associated virus (AAV) is a common delivery vehicle in gene therapy. Robust, rapid and automated characterization methods are imperative for commercialization of AAV-based products. However, standard methods are labor- and time-intensive, requiring costly reagents. In this presentation, we will discuss a method to measure three critical quality attributes (CQAs) of AAVs: 1) total number of viral capsid particles; 2) relative capsid content (e.g., fraction of full capsids); and 3) percentage of monomer or aggregates. The highly precise SEC-MALS method utilizes multi-angle light scattering in line with standard size-exclusion chromatography, requiring little sample prep, no reagents and minimal operator effort.

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AAV-1500×843

Using Chemical Denaturants and Light Scattering to Determine Aggregation Propensity of Biopharmaceuticals

Join us for a webinar on Wednesday, October 27 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

Determining the developability of candidate molecules and optimizing formulations require rapid assessment of biophysical characteristics using accelerated stressors to represent normal storage conditions. Thermal stress, assuming the Arrhenius relationship between temperature and degradation rate (which has been successfully used for small molecule development), is the most common approach. Unfortunately, this relationship is not straightforward for biological molecules, so alternative approaches, such as using denaturants to accelerate non-native interactions, could prove beneficial.

Static and dynamic light scattering are invaluable tools for quantifying protein-protein interactions and aggregation phenomena. This webinar will review light scattering-based methods for protein characterization and explain their utility, when combined with partially denaturing buffers, in understanding aggregation propensity of monoclonal antibodies (mAbs).

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mAbs

Upcoming Rebroadcasts w/ Live Q&A

October 20, 10-11 AM (CEST): Analysis of Viral Proteins, Nucleic Acids and Biomolecular Interactions with Light
Characterizing viral proteins and their interactions with receptors and engineered monoclonal antibodies (mAbs) is key to fundamental understanding of how a virus infects cells and ultimately to the successful development of protein-based drugs. Light-scattering based methods provide essential solutions to addressing these questions.

November 17, 10-11 (CET): Understanding Polymer Properties with Multi-Angle Light Scattering
Polymers, both of synthetic and natural origin, are an integral part of our everyday life. In contrast to low-molar-mass organic compounds, polymer samples of identical chemical composition can differ substantially by their application properties. Many important polymer properties are affected by the molar mass distribution and structural topology of the polymer chains. A multi-angle light scattering (MALS) detector following an analytical separation technique such as SEC or FFF, often in combination with an online viscometer, can provide detailed information about the molecular structure and thus proper understanding of the properties and behavior of a very wide range of polymers.

Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

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