Upcoming Live Webinars
Advances in Downstream PAT for Biologics, Vaccines and Gene Vectors
Join us for a webinar on Tuesday, August 23 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
Rapid process development and scale-up of novel complex drugs, as well as reliable unit operations during commercial production, require advanced PAT tools, capable of providing timely feedback on product attributes. Real-time multi-angle light scattering (RT-MALS) determines key biophysical product attributes, including molar mass, particle size and particle concentration. These attributes are further indicative of quality attributes such as titer, aggregation and full-empty ratio, and may be used for process control as well as quality monitoring.
This webcast will review the principles, capabilities and limitations of RT-MALS, and then present case studies covering downstream processing (DSP) of viral vectors, proteins and polysaccharides, as well as formulation, production and purification of lipid nanoparticles.
Comparing Methods that Determine Empty-Full Ratio of AAV Capsids
Join us for a webinar on Tuesday, September 20 from 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
The relative abundance of empty and full AAV capsids in gene therapy vector preparations is a critical quality attribute. A variety of methods are available to perform these measurements including SEC-MALS, which combines separation by size-exclusion chromatography with detection by multi-angle light scattering, UV/Vis, and differential refractometry. However, there are relatively few reports that compare these methods. This webinar compares the outcomes of numerous orthogonal characterization methods on a single vector preparation. We find that SEC-MALS not only provides favorable performance for quality control and product development applications, but that it is uniquely situated to be established as a platform method for the semi-quantitative measurement of empty/full ratios across AAV serotypes, transgenes, and manufacturing processes.
Advantages of FFF-MALS over SEC-MALS for the Determination of Molar Mass and Size Distribution of Polysaccharides
Join us for a webinar on Tuesday, September 27 at 10:00 AM CEST
Size-exclusion chromatography (SEC) has been widely used for the determination of molar mass and molar mass distribution of various natural biopolymers. However, the lack of appropriate calibration standards and non-ideal separation due to column interactions often causes misleading and incorrect results.
This webinar explores the capabilities of the two separation techniques SEC and field-flow fractionation (FFF) for characterizing the molar mass, size as well as distribution of sulfated polysaccharides extracted from brown algae, none as fucoidan. Most of the non-ideal separation can be eliminated by applying the alternative separation technique FFF.
Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:
Vaccines Illuminated: Biophysical characterization, PAT, and quality control via light scattering techniques
Sophia Kenrick, Ph.D., Wyatt Technology
July 20, 2022
Experimental methodology in a core facility: Light scattering for preliminary experiments and final answers
Peter Randolph, Ph.D., Florida State University and Landry Bennett, Ph.D., Wyatt Technology
June 21, 2022
Jing Lan, Wyatt Technology
April 29, 2022
Tao Lei Zhao, Wyatt Technology
May 12, 2022
Stability characterization of vaccines, gene vectors and therapeutic antibodies with light scattering
Xujun Zhang, Ph.D., Wyatt Technology
May 24, 2022
Solving SEC/GPC Limitations with Multi-Angle Light Scattering and Asymmetric-Flow Field-Flow Fractionation
Stepan Podzimek, Ph.D., Wyatt Technology
April 19, 2022
Adam Gormley, Ph.D., Rutgers University, and Eric Seymour, Wyatt Technology
March 24, 2022
Using SEC-MALS in Protein Characterization and Structural Studies in the Biocenter Oulu Structural Biology Core Facility
Kristian Koski, Ph.D., Biocenter Oulu, University of Oulu
March 22, 2022
Looking at proteins from different angles: The unusual behavior of phospholipase PlaB from Legionella pneumophila
Wulf Blankenfeldt, Ph.D., Helmholtz-Zentrum für Infektionsforschung
February 22, 2022
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