Upcoming Live Webinars
Real-Time Monitoring of AAV CQAs in a Chromatographic Polishing Step
Join us for a webinar on Tuesday, April 9 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CET
Downstream purification and enrichment of full capsids for AAV-based gene therapy products, along with aggregate removal, is typically accomplished by ion-exchange chromatography (IEX). Development of this process usually requires sending multiple fractions for offline analysis for each iteration of process conditions, creating a bottleneck that holds back time-to-market and burdens both the development and analytical teams.
Real-time multi-angle light scattering (RT-MALS) is a process analytical technique that quantifies biophysical attributes of AAVs including Vg/Cp, titer and aggregation. RT-MALS provides immediate, high-density feedback on critical quality attributes (CQAs) during the chromatography run, eliminating burdensome offline analyses, and enables optimized control over the collection of purified product. This webcast will present RT-MALS basics and case studies for accelerated AAV process development in a CDMO.
Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of your Results.
Join us for a webinar on Thursday, April 18 at 7:00 AM PDT | 10:00 AM EDT | 15:00 CET
The scientific community aims to enhance transparency and reliability in published research, with a specific focus on the quality of biological reagents, notably recombinant proteins. Concerns have been raised regarding irreproducible results, prompting discussions on common quality issues associated with recombinant proteins and their impact on downstream applications. In response, professionals in protein purification and characterization have collaboratively developed guidelines for improved quality control.
These guidelines include minimal information requirements for protein identity, production parameters, and long-term stability, as well as a minimal set of quality tests for purity, homogeneity, and identity. Additional recommendations cover DNA contamination, “spectral and thermal denaturation signatures”, homogeneity, “competent fraction”, storage conditions, batch-to-batch reproducibility, and other factors based on intended applications. Evaluation of these guidelines over a one-year period suggests that their implementation can enhance experimental reliability and optimize protein quality. Investing in protein QC benefits all stakeholders in life sciences, including researchers, editors, and funding agencies, by improving data veracity and minimizing resource wastage.
Recent Webinars
Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:
Request: Exploring Enhanced Macromolecular and Nanoparticle Analysis through the Integration of DLS/ELS/SLS with the ZetaStar Instrument
Sophia Kenrick, Ph.D., Waters | Wyatt Technology, and Richard Chung, Waters | Wyatt Technology
March 15, 2024Request: Leveraging Advanced Analytical Technologies to Expedite the Development of Antibody Drug Conjugates
Eric Miller, Ph.D., Abzena, and Colette Quinn, Ph.D., Waters | Wyatt Technology
February 28, 2024Request: Supporting Sustainability: A Comprehensive Case Study on PLGA and PLA Polymer Characterization and Optimization Using Light Scattering with Triple Detection
Stepan Podzimek, Ph.D., Waters | Wyatt Technology
February 13, 2024Request: Smaller Particle and Low Adsorption Columns for Fast and Efficient Characterization of AAV through SEC-MALS
Bala Addepalli, Ph.D., Waters
January 31, 2024Request: Towards Well-Characterized Proteins and Protein Conjugates Using SEC-MALS
Michelle Chen, Ph.D., Waters | Wyatt Technology
January 17, 2024Request: In-Line and On-Line Monitoring of CQAs for Biologics, Vaccines and Gene Vectors
Daniel Some, Ph.D., Waters | Wyatt Technology
December 5, 2023Request: Successful SEC-MALS analysis of proteins and AAVS
Leo Liu, Ph.D., Waters | Wyatt Technology
July 11, 2023 & November 22, 2023Request: Determination Critical Product Parameters for Proteins from Research to Production
David Golonka, Ph.D., Waters | Wyatt Technology, and Felix Deluweit, Ph.D., Waters | Wyatt Technology
November 8, 2023Request: Multi-Attribute Quantification of Viral Vectors Using Light Scattering
Martin Kurnik, Ph.D., Waters | Wyatt Technology
November 1, 2023Request: Optimizing Development and Release Efficiency of Protein, AAV and LNP Therapies Using Multi-Attribute Quantification (MAQ) Light Scattering
Michelle Chen, Ph.D., Waters | Wyatt Technology
November 1, 2023Request: Shining Light on AAVs: Using Light Scattering Technologies to Solve Challenges in Characterization
Steven Milian, M.S., Thermo Fisher Scientific, Gennarino Del Bagno, Thermo Fisher Scientific, and Sophia Kenrick, Ph.D., Waters | Wyatt Technology
October 18, 2023Request: Advances in Automated Dynamic Light Scattering Characterization and Zeta Potential of Macromolecules and Nanoparticles
Sophia Kenrick, Ph.D., Waters | Wyatt Technology and Richard Chung, Waters | Wyatt Technology
October 10, 2023Request: Unraveling the Complexity: Advanced Analysis of Polymeric Nanostructures
Susanne Boye, Ph.D., Institut Makromolekulare Chemie and Robert Mildner, Ph.D., Waters | Wyatt Technology
August 29, 2023
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