Boston AAV User Meeting & Workshop
Join us at the Hilton Boston/Woburn
November 19th Boston AAV User Meeting & Workshop
AAV Workshop: An introduction to the characterization of AAV by light scattering
Adeno-associated virus (AAV) is an attractive delivery vehicle in gene therapy attributed to its mild immune response and ability to deliver the therapeutic genome into a wide range of host cells. To ensure the safety and efficacy of the viral vector-based drug product, it is essential to implement robust and reliable characterization tools throughout the production and manufacturing process. In this presentation, we will discuss a size exclusion chromatography (SEC) method coupled with UV, multi-angle light scattering (MALS), and differential refractive index (dRI) detectors to measure the following three important AAV quality attributes (QAs): 1) total number of viral capsid particles; 2) relative capsid content (e.g., ratio of empty and full capsids); and 3) percentage of monomer or aggregates. Additionally, orthogonal methods using dynamic light scattering (DLS) and field flow fractionation (FFF) will be compared.
AAV User Meeting: Secrets to the successful quantification of AAV by light scattering
We will provide step-by-step training on how to use SEC-UV-MALS-dRI, protein conjugate analysis in ASTRA software, and additional calculations to measure AAV quality attributes (QAs). The QAs include: 1) total number of viral capsid particles; 2) relative capsid content (e.g., ratio of empty and full capsids); and 3) percentage of monomer or aggregates. We will also be available to answer any of your questions regarding DLS and FFF applications and analysis.
Date: November 19, 2019: AAV Workshop
AAV Workshop Time: 8:30 AM – 11:00 AM
AAV User Meeting Time: 11:00 AM – 4:15 PM
Location: Hilton Boston/Woburn
2 Forbes Rd.
Woburn, MA 01801
Who should attend:
- Workshop: Anyone who is interested in tools to characterize and quantify AAV and other viral vectors for gene therapy.
- User Meeting: Active SEC-MALS users who want to learn how to characterize and quantify QAs of AAV particles and other viral vectors.
Registration is free. Continential breakfast and lunch are provided.
September 2019 Meeting was held in Woburn, MA
April 2019 Meeting was held in Woburn, MA
April 2018 Meetings were held in Woburn, MA
- Julia Deuel, Bluebird Bio
"Biophysical Characterization of a Complex Product: Lentiviral Vector"
- Damian Houde, Codiak Biosciences
"Characterization of extracellular vesicles by light scattering techniques"
- Haiying Zhang, Weill Cornell Medical College
"Identification of a novel nanoparticle population and distinct subsets of exosomes by AF4"
April 2016 Meeting was held in Woburn, MA
- Wing-Yee Fu, Momenta Pharmaceuticals
"SEC-MALS for the Characterization of a Complex Generic Drug-Glatiramer Acetate"
- Chris Broomell, Takeda Vaccines
"Bringing Understanding to What's Underneath the Peak: Light Scattering's Contribution to a Well Characterized VLP Vaccine"
April 2015 Meeting was held in Woburn, MA
- Elizabeth Bartlett, Immunogen
"Investigation of Reversible Self-Association During Early Stage Development of a Low Concentration Antibody-Drug Conjugate"
- Patricia Lowden, Eleven Therapeutics
"Colloidal Stability of Proteins by Self-Interaction Nanoparticle Spectroscopy and Dynamic Light Scattering"
- Allie Obermeyer, MIT
"Light Scattering to Study the Interaction of Proteins and Polyelectrolytes"
- Jonathan Kingsbury, Sanofi
"Measuring Weak Interactions in Protein Solutions by Light Scattering and Sedimentation"
November 2013 Meeting was held in Woburn, MA
- Ewa Folta-Stogniew, Yale University
"Protein Interactions Studied by Light Scattering"
- Nicholas Luksha, Pfizer Inc.
"Utilization of Light Scattering Techniques as Screening Tools for Formulation Development"
- Dominic Walsh, Brigham & Women's Hospital & Harvard Medical School
"Alzheimer's Disease and the Amyloid β-protein – Size Matters"
- John Champagne, Wyatt Technology
"VLP Characterization: Molar Mass, Size, Composition, Conformation, and Interactions"
- Dan Some, Wyatt Technology
"CG-MALS Case Studies: 1. Hetero-association - Multi-Valent Protein-Ligand Binding, 2. Self-Association - Concentrated IgG"
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