Raleigh AAV Workshop & Advanced Training
Join us in the RTP area to learn about AAV analysis by light scattering on March 27!
Adeno-associated virus (AAV)-based viral vectors have emerged as popular delivery vehicles in gene therapy owing to their lack of pathogenicity, low immunogenicity, and ability to deliver therapeutic genomes into a wide range of host cells. To ensure the safety and efficacy of viral vector-based drug products, it is essential to implement robust and reliable characterization and quantitation analytics throughout the development and manufacturing process. Join us for one or both these events on Monday, March 27, to learn more.
AAV Workshop: Industrial case studies and introduction to quantifying AAV CQAs by light scattering
In this workshop, we will discuss the SEC-MALS method for measuring multiple CQAs in a single 30-min run and with an LOQ of 5x1010 mL-1. These CQAs include Cp (total titer), Vg, E/F, and percentage of small aggregates. Strategies for using SEC-MALS in QC as a release assay will also be shared in detail. Additionally, AAV solutions using DLS (for screening size, particle concentration, and stability), FFF (for quantifying all aggregates), and in-line monitoring (for process development) tools will be presented as well.
The workshop is open to anyone who is interested in learning more about light scattering tools for AAV analysis. Admission is free, and complimentary continental breakfast is included.
AAV Advanced Training: How to quantify AAV CQAs by light scattering
We will provide step-by-step training on how to use SEC-MALS and Wyatt’s Viral Vector Analysis to quantify AAV CQAs using the AAV SOP Guidance Manual as reference. We will also provide guidance on best practices on using DLS and FFF-MALS for AAV analysis. At the end of the meeting, we will offer data review opportunities to those who do not mind staying late. Admission is free, and a complimentary lunch will be provided.
Monday, March 27, 2023
AAV Workshop: 8:30 AM – 10:30 AM
AAV Advanced Training: 11:00 AM – 4:30 PM
Who should attend:
- Workshop: Anyone who is interested in tools to characterize and quantify AAV and other gene vectors.
- Advanced Training: Anyone who is interested in or actively working with SEC-MALS who want to learn how to quantify CQAs and perform extended characterization of AAV products.
Workshop registration is complimentary to all and includes continental breakfast and lunch.