AN2002: Enzymatically Cross-linked Casein Characterized by FFF-MALS

This application note shows the capabilities of asymmetric-flow field-flow fractionation (AF4) in combination with differential refractive index measurement plus static and dynamic light scattering (AF4-MALS-DLS) for a gentle separation and investigation of caseins enzymatically cross-linked with mTGase. Using this approach we have gained essential knowledge on the modus operandi of mTGase on size and conformation of caseins.