DNA and RNA Repair Enzymes

ABH3, one of the DNA and RNA repair enzymes in humans, directly reverses methylated lesions into unmethylated bases. The activity of ABH3 is important in keeping the integrity of the human genome; therefore, obtaining the crystal structure of this protein will give insights into bio-informatics studies for other DNA repair enzymes, and help to design future enzyme inhibitors. Achieving protein stability and homogeneity is crucial for obtaining protein crystals. In this experiment, a DynaPro^® light scattering instrument was used to monitor the protein stability and homogeneity in different buffers.