Request: Vaccines Illuminated: Biophysical characterization, PAT, and quality control via light scattering techniques
Key Learning Objectives:
- How molar mass, size, titer, and composition of therapeutic and vaccine biomolecules are determined with size-exclusion chromatography coupled to multi-angle light scattering (SEC–MALS)
- How mRNA-lipid nanoparticle vaccines are characterized with field-flow fractionation coupled to MALS (FFF–MALS)
- How quality attributes of viruses and virus-like particles are quantified using MALS and dynamic light scattering (DLS)
- How high-throughput formulation and stability studies of are carried out with plate-based DLS
- How real-time MALS can monitor chromatographic purification of viruses and VLPs