Vaccines Illuminated: Biophysical characterization, PAT, and quality control via light scattering techniques request

Sophia Kendrick

Key Learning Objectives:

  • How molar mass, size, titer, and composition of therapeutic and vaccine biomolecules are determined with size-exclusion chromatography coupled to multi-angle light scattering (SEC–MALS)
  • How mRNA-lipid nanoparticle vaccines are characterized with field-flow fractionation coupled to MALS (FFF–MALS)
  • How quality attributes of viruses and virus-like particles are quantified using MALS and dynamic light scattering (DLS)
  • How high-throughput formulation and stability studies of are carried out with plate-based DLS
  • How real-time MALS can monitor chromatographic purification of viruses and VLPs