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Structural Characterization of Biopolymers by Analytical Separation Techniques with Advanced Detectors


Presented Live: May 9, 2019

Speakers: Stepan Podzimek, Ph.D.

This webcast explores the capabilities of multi-detector analysis of biopolymers after separation by size-exclusion chromatography or field-flow fractionation. The primary detection technologies include multi-angle light scattering and differential viscometry, along with traditional detectors such as refractive index and UV–vis. Case studies include difficult samples such as branched and other nonlinear polymers, and fluorescent polymers.


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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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