Wyatt Technology

DynaPro Plate Reader III

DynaPro Plate Reader III

 

 

High-throughput, automated, Dynamic and Static Light Scattering

Revolutionize your performance by replacing your conventional, cuvette-based dynamic light scattering unit with the automated DynaPro® Plate Reader III. The DynaPro excels at high-throughput sizing and screening of pharmaceutical and nanoparticle formulations to enable novel workflows that simply would not be possible with other DLS instruments. Even if you need to run just a handful of samples, it's a great productivity booster.

For biotherapeutic development, the DynaPro Plate Reader III is a comprehensive solution for stability evaluation. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (Tonset, Tm, Tagg) and colloidal stability (A2/B22, kD) may be quantified with plate-based DLS and SLS. Even viscosity of concentrated protein solutions can be screened across dozens of formulations in a single plate.

Included with each DynaPro Plate Reader is a copy of DYNAMICS, our powerful software package for analyzing automated batch dynamic light scattering. The Security Pack (DYNAMICS SP) option provides 21 CFR (11) compliance for GMP/GLP use.

  • Revolutionize DLS

    The DynaPro Plate Reader III offers dynamic light scattering (DLS) and static light scattering (SLS) measurements of the size, molecular weight and interactions of proteins, nanoparticles and other macromolecules in situ in industry-standard microwell plates. No liquid handling occurs after dispensing into the plates, to maximize throughput and minimize sample carryover. This ground-breaking instrument provides rapid optimization of the formulation process for:

    A wealth of applications

    The DynaPro Plate Reader III provides unparalleled levels of ease-of-use, productivity, reproducibility, and flexibility in order to address additional demanding applications such as:

    • Optimizing protein crystallization
    • Assessing aggregation or oligomerization
    • Determining protein stability-indicating parameters such as thermal denaturation and colloidal stability
    • Screening high-concentration protein solutions for reversible association and viscosity

    Key features

    • Measure hydrodynamic radius from 0.5 nm to 1000 nm
    • Sensitivity for size down to 0.125 mg/mL lysozyme
    • Measure weight-average molar mass from 1 to 1000 kDa
    • Industry standard microwell plates of 96, 384 or 1536 wells
    • Sample volume from 4 μL to 150 μL
    • All measurements made in situ in the wells
    • Temperature ramps from 4°C to 85°C
    • Integrates with plate-based liquid handling robotics

  •  

    • A wealth of features

      • Rapid or slow temperature ramps 4°C - 85°C with excellent uniformity across the plates. Samples are heated in parallel and all filled wells measured at each temperature.
      • Well read times as short as 5-10 seconds
      • Seal wells with oil or sealing tape to prevent evaporation. The tape is gently heated to prevent condensation.
      • Integration with liquid-handling robots for higher levels of automation, especially when your workflow is plate-based, and utilizes spectroscopic and other plate readers in addition to DLS/SLS
      • A camera for viewing each well from below, to aid in data diagnostics (e.g. to detect precipitation or bubble formation)
    • A set of images collected by the onboard camera, showing clean wells and wells containing bubbles, crystals and precipitates.

    • Lab automation

      An OLE software API is available for interfacing the DynaPro Plate Reader II to robotic liquid handling in the high-throughput lab. This capability allows for fully automated plate loading and data collection.

      Wyatt partners with several companies that offer integrated solutions for automated loading and analysis of plated samples: 


    • Hudson Robotics Plate Transfer

      Click to play video

         Contact Hudson Robotics

    • In addition, Hamilton Robotics, Caliper Life Sciences, and Tecan offer custom integration services.

  • Compatible Well Plates

    NanoFilter Kit - Makes it easy to filter, recover and refilter precious sample. This is especially useful when your total sample volumes are below 300 µL.

  • DYNAMICS 7, software

    DYNAMICS - Automates well-plate scans, temperature ramps, etc. and analyzes DLS data to determine size distributions, temperature onset of melting or aggregation, second virial coefficient A2and the diffusion interaction parameter kD.

    DYNAMICS is offered with a 21CFR(11)-compliant Security Pack enabling the use of the DynaPro Plate Reader in a GMP environment.

  • Supported Well Plate Formats 96, 384 or 1536 *
    Dynamic Light Scattering
    Size Range (Radius, Rh) 0.5 to 1000 nm
    Minimum Concentration @ 14 kDa 0.125 mg/mL
    Static Light Scattering
    Molar Mass Range 1000 to 1,000,000 Da
    Minimum Concentration @ 67 kDa 1 mg/mL ††
    Optics
       Laser Wavelength 830 nm
       Laser Power Control Programmable 10% – 100%
       Attenuation Range 1 to 105
    Temperature Control 4 – 85°C †††
    Fluidics
       Minimum Sample Volume 4 µL ††††
    Data Acquisition Time 1 to 3600 seconds per well per read
    Read Time per Well 5 – 20 seconds
    Electronics
       Correlator 512 channels †††††
       Onboard Camera 3 megapixels ††††††
       Digital Communication Ethernet (TCP/IP)
    Dimensions 60 cm (L) x 36 cm (W) x 25 cm (H)

    * Supports many industry standard well plates. Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. for a list of supported plates.

    Minimum concentration specified with 50 µL of Lysozyme (14 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

    †† Minimum concentration specified with 50 µL of BSA (67 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

    ††† Accuracy of ± 0.5°C for 4 – 50°C, and ± 1°C for 50 – 80°C. Minimum temperature of 4°C requires a laboratory temperature of 24°C or below.

    †††† Minimum volume specified with 2 mg/mL Lysozyme in a 1536 well plate, Wyatt P/N P8803-1536, with 25 seconds acquisition.

    ††††† 100 nsec sampling time in a multi-tau layout

    †††††† Camera operates with sample temperature in the range of 4 – 50°C, and automatically powers off above 50°C.


    Host PC requirements may be found in Computer Requirements.

    Specifications subject to change without notice.

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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