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Branching Calculations

When using a DAWN or miniDAWN to make molar mass measurements, it is possible to perform sophisticated branching calculations to determine the branching of a polymer sample. ASTRA can determine the degree of tri- or tetrafunctional branching, and even analyze star and comb polymers. If the molar mass and rms radius for a linear and corresponding branched polymer are measured, then ASTRA can calculate the following quantities:

  • branching ratio gM .
  • branch units per molecule.
  • long chain branching.

In addition, if the polymer is too small (rms radius < 10 nm) for size measurements using light scattering, ASTRA can use the mass method for samples with known Mark-Houwink-Sakurada parameters and common elution times.

Branching Analysis GUI

The branching analysis view puts everything at your fingertips to make branching calculations simple. Choose between four different polymer types: tri- or tetra-functional, star, or comb branching. Enter parameters for long chain branching, or the mass method. Finally, instantly view results by selecting the plot type. Displayed at left is the interface with a comparison of conformation plots and slopes for a linear and branched sample.

The following plots demonstrate the other visual information available in the branching analysis view:

  • Branching Ratio
  • Branch Units per Molecule

  • long chain branching



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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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