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On-Line QELS

ASTRA supports data collection and analysis from the WyattQELS DLS Module and certain DynaPro instruments. When analyzing QELS data, ASTRA can:

  • Measure the translational diffusion and corresponding hydrodynamic radius of each eluting fraction in an online measurement.
  • Determine integral and differential distributions of the translational diffusion coefficients and hydrodynamic radii.
  • Calculate the ratio of the hydrodynamic radius (Rh) and rms radius for additional conformation information.
  • Present the full correlation function for inspection and analysis.
  • Calculate uncertainties in measured quantities.
QELS Analysis GUI
The QELS analysis view in ASTRA presents the correlation function and corresponding single exponential fit. Analysis parameters can be set in the view, with immediate feedback in the results and fit.

Custom plots can be made showing not only the Rh value as a function of elution volume, but also the uncertainties in the presented quantities. Consider the plot of Rh vs. elution volume for a fractionated BSA sequence.

QELS Analysis of BSA



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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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