The unique combination of FFF-MALS-DLS
Our detectors for multi-angle light scattering and refractive index represent the leading technology in their categories. When combined, they create an all-new level of capability that dramatically expands your characterization capabilities.
The Eclipse® DualTec supports both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow FFF (AF4) in one instrument. Utilizing one HPLC stack and one set of Wyatt detectors, the DualTec permits automated switching between any two of SEC, AF4 and HF5 separation followed by MALS and/or DLS characterization.
DAWN® HELEOS® II
The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.
A unique on-line differential refractometer for measuring concentration of any macromolecule, regardless of chromophores. The high-concentration Optilab accommodates protein concentration up to 180 mg/mL.
Isolation and Characterization of Extracellular Vesicles (EVs)
Field-flow fractionation combined with multi-angle and dynamic light scattering (FFF-MALS-DLS) has increasingly received recognition as a key tool in extracellular vesicle research, especially for exosomes. It is evident that the differences in size, surface molecules and chemistry, and mechanical properties among exosome subpopulations all collectively contribute to their biodistribution and systemic functions in vivo. FFF-MALS-DLS combines true size-based separation using an Eclipse® FFF system with independent determination of size and structure by a DAWN® HELEOS® II light scattering instrument. It can determine highly accurate size distributions and particle concentrations in native solution and the size fractions may be isolated and automatically collected for further off-line investigation using a standard fraction collector.