Upcoming Live Webinars

Real-Time Monitoring of AAV CQAs in a Chromatographic Polishing Step

Join us for a webinar on Tuesday, April 9 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CET

Downstream purification and enrichment of full capsids for AAV-based gene therapy products, along with aggregate removal, is typically accomplished by ion-exchange chromatography (IEX). Development of this process usually requires sending multiple fractions for offline analysis for each iteration of process conditions, creating a bottleneck that holds back time-to-market and burdens both the development and analytical teams.

Real-time multi-angle light scattering (RT-MALS) is a process analytical technique that quantifies biophysical attributes of AAVs including Vg/Cp, titer and aggregation. RT-MALS provides immediate, high-density feedback on critical quality attributes (CQAs) during the chromatography run, eliminating burdensome offline analyses, and enables optimized control over the collection of purified product. This webcast will present RT-MALS basics and case studies for accelerated AAV process development in a CDMO.

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Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of your Results.

Join us for a webinar on Thursday, April 18 at 7:00 AM PDT | 10:00 AM EDT | 15:00 CET

The scientific community aims to enhance transparency and reliability in published research, with a specific focus on the quality of biological reagents, notably recombinant proteins. Concerns have been raised regarding irreproducible results, prompting discussions on common quality issues associated with recombinant proteins and their impact on downstream applications. In response, professionals in protein purification and characterization have collaboratively developed guidelines for improved quality control.

These guidelines include minimal information requirements for protein identity, production parameters, and long-term stability, as well as a minimal set of quality tests for purity, homogeneity, and identity. Additional recommendations cover DNA contamination, “spectral and thermal denaturation signatures”, homogeneity, “competent fraction”, storage conditions, batch-to-batch reproducibility, and other factors based on intended applications. Evaluation of these guidelines over a one-year period suggests that their implementation can enhance experimental reliability and optimize protein quality. Investing in protein QC benefits all stakeholders in life sciences, including researchers, editors, and funding agencies, by improving data veracity and minimizing resource wastage.

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Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

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