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  • Pacifichem 2015THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES

    Preparation, Measurement, and Fractionation of Monodisperse Single Wall Carbon Nanotubes

    presented by Dr. Philip Wyatt, Chief Executive Officer
    December 19, 2015 | 11:10 - 11:30 AM

    The many extant and growing applications of single wall carbon nanotubes (SWCNT) suggest strongly that care must be exercised to insure that each such application includes a physical description of the nanotubes used as well as a specification of the properties best suited for the application being studied. One of the most important features of these nanotubes is their length. Assuming that they are not aggregated, it remains to establish for subsequent use that their lengths are known accurately and that each sample is comprised of nanotubes of the same length, i.e. the sample is monodisperse. Using Wahlund chromatographic separation (also known as A4F), traditionally prepared SWCNT samples are fractionated and each fraction sized. Using newly developed analytical techniques, the length of such fractions may be determined during the separation process. Specific size subsets then may be segregated as they elute insuring, thereby, that the nanotube samples to be used are monodisperse. Preparation protocols may be differentiated and quantified, for example, on the basis of the differential size distributions they yield.

    Place: Honolulu, HI
    For more info: Pacifichem 2015 Meeting

     


    CHI's PepTalk 2016

    Molar mass, size and interactions: light scattering tools for essential biophysical characterization

    Presented by Dr. Wafa Hassouneh, Applications Scientist
    Thursday, January 21, 2016 3:05 PM

    Biophysical techniques based on static and dynamic light scattering address many of the key analytical challenges in biotherapeutic research and development, from early candidate selection through scale-up, formulation, characterization and comparability studies. This seminar will review light scattering technology and instrumentation, then present select examples illustrating how Wyatt's light scattering solutions facilitate rapid and effective development of biologics including mAbs, ADCs, PEGylated and other proteins.

    Exhibitor RibbonVisit us at Booth #413

    Place: San Diego, CA
    For more info: 2016 PepTalk Annual Conference

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    WCBP 2016

    At-line and off: The Light Scattering Toolkit for Essential Biophysical Characterization

    Presented by Dr. Daniel Some, Director of Marketing & Principal Scientist
    Wednesday, January 27, 2016 10:20 AM

    From aggregates to conjugates and peptides to polysaccharides, light scattering detectors combine with a variety of sample delivery systems to provide a comprehensive suite of biophysical characterization tools. The latest addition to the toolkit, the µDAWN, adds multi-angle light scattering capabilities to UHPLC for enhanced resolution, speed and productivity in absolute molar mass determination. This seminar presents an overview of instrumentation and examples of the tasks relevant to well-characterized biopharmaceuticals that may be accomplished with these tools, with a look at potential for use in-line and at-line on the production floor.

    Exhibitor RibbonVisit us at Booth #26

    Place: Palm Court Room, Mayflower Hotel, Washington, DC
    For more info: 2016 WCBP Conference

     


    Biophysical Society Annual Meeting 2016

    Get it Right the First Time - Enhancing Protein Binding and Structural Studies with the Light-Scattering Toolkit

    Presented by Dr. Sophia Kenrick, Application Scientist
    Monday, February 29, 2016 10:30 AM

    Biophysical binding studies utilizing surface plasmon resonance (SPR), biolayer interferometry (BLI), isothermal titration calorimetry (ITC), and related techniques are central to the study of protein-protein, protein-DNA and similar biomolecular interactions. Though these are well-established techniques, in a variety of circumstances, binding measurements may be ambiguous or even fail to provide useful data. Wasted measurements can end up being costly in terms of consumables and time. Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) are powerful techniques for studying biomolecular structure. SAXS and SANS usually require precious beam time at large facilities, leaving little room for error where the sample preparation is concerned. Poor samples provide poor SAXS/SANS data, but the opportunity to utilize the X-ray or neutron beam may never be recovered. One thing that SAXS and SANS have in common with SPR, BLI and ITC, is the urgent need to verify sample quality and aggregation state in solution prior to carrying out structural or binding measurements. This seminar discusses a suite of complementary techniques, all based on light scattering, that are useful in assessing and troubleshooting many of the underlying characterization issues. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) can help researchers assess solution quality prior to running binding or structural experiments, qualify aggregation behavior of analytes, and characterize complex interactions that may not be amenable to standard characterization methodology. Judicious use of the biophysical light-scattering toolkit is essential for robust and reliable interaction and structure studies.

    Exhibitor RibbonVisit us at Booth #601

    Place: Los Angeles, CA
    For more info: 2016 Biophysical Society Annual Conference

     


    Pittcon 2016

    SHORT COURSE: Light Scattering Techniques for Protein, Polymer, and Characterization

    Presented by Dr. Sigrid Kuebler, Director of Customer Service and Support
    Wednesday, March 9, 2016 8:30 AM

    Light scattering techniques are commonly used to characterize macromolecules in terms of their absolute molecular weight, size and second virial coefficient. This seminar will provide an overview of the fundamentals and applications of Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). Both techniques can be applied either in stand-alone (batch) mode as well as coupled to a separation technique, e.g. size-exclusion chromatography (SEC) or field flow fractionation (FFF). When used with a separation system, light scattering detection can additionally be used for the assessment of size heterogeneity (aggregation and fragmentation), molecular weight distribution, compositional heterogeneity (protein conjugates and copolymers), conformational analysis, and branching. Scientists in the pharmaceutical, chemical, biotechnology and medical device industries, and researchers working in academic or government labs, who wish to learn how light scattering can help them better characterize peptides, proteins, (bio)polymers or nanoparticles. This is an introductory course - no previous light scatting experience is required.

    Exhibitor RibbonVisit us at Booth #3630

    Place: Georgia World Conference Center, Atlanta, GA
    For more info and to Register: 2016 Pittcon Short Course

     


  • Wyatt Technology Europe's Spring Seminar Series:
    How Wyatt Technology’s Light Scattering Toolkit Contributes to the Characterization of Proteins

    Presented by

    Dr. Dierk Roessner, Senior Application Scientist, Wyatt Technology Europe
    Dr. Roger Scherrers, Senior Application Scientist, Wyatt Technology Europe
    Dr. Thomas Jocks, Senior Scientist Marketing, Wyatt Technology Europe

    In these seminars we will discuss the characterization of proteins by determination of their size heterogeneity and molecular weight distribution (aggregation and fragmentation), their compositional heterogeneity (modified proteins, membrane proteins, protein complexes), and conformational analysis.

    Light scattering detectors can be combined with a variety of sample separation and delivery systems such as Size Exclusion Chromatography and Field Flow Fractionation, or applied for batch methods to provide a comprehensive suite of biophysical characterization tools. The latest offspring which recently joined the Wyatt toolkit, the µDAWN, adds multi-angle light scattering capabilities to UHPLC for enhanced resolution, speed and productivity in absolute molar mass determination.

    These seminars give an overview of the basic light scattering principles and introduce the instrumentation.

    Based on practical examples we present the most promising approaches towards well-characterized proteins and biopharmaceuticals. We also take a look at the potential to use these techniques in-line and at-line in the production process.

    Who should attend?

    Scientists in the pharmaceutical, chemical, biotechnology and medical device industries, and researchers working in academic or governmental labs, who wish to learn how light scattering can help them to comprehensively characterize their peptides and proteins. As this is an introductory seminar, no profound light scattering experience is required.

    For more information please contact This email address is being protected from spambots. You need JavaScript enabled to view it..

    Register Online

    Seminar Locations, Dates and Times

    Wyatt Technology Europe at the University of Zürich

    Wednesday, February 17, 2016, 8:30 h to 15:30 h

    The seminar is hosted by Zürich University’s Department of Biochemistry.

    Several young scientists from the working groups of Prof. Plückthun and Prof. Zerbe will contribute to the seminar with intriguing insights into their recent work.

    For more information please contact This email address is being protected from spambots. You need JavaScript enabled to view it..

    Wyatt light scattering seminar Zurich

     

    Wyatt Technology Europe at the University of Freiburg

    Thursday, February 18, 2016, 8:30 h to 15:30 h

    The seminar is hosted by the Institute for Pharmaceutical Sciences at the University of Freiburg/Br.

    Guest Speaker TBA

    For more information please contact This email address is being protected from spambots. You need JavaScript enabled to view it..

    Wyatt light scattering seminar Freiburg

     

    Wyatt Technology Europe at the University of Tübingen

    Friday, February 19, 2016, 9:00 h to 15:00 h

    The seminar is hosted by the Institute for Microbiology at the University of Tübingen.

    Prof. Dr. Karl Forchhammer will give an intriguing talk on his recent work, preliminarily entitled: "Glutamine Synthetase from Bacillus subtilis: a complex enzyme with dual function"

    For more information please contact This email address is being protected from spambots. You need JavaScript enabled to view it..

    Wyatt light scattering seminar Tubingen

     

 

For additional information on all seminars, please contact This email address is being protected from spambots. You need JavaScript enabled to view it.

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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