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  • GGPF 2017

    To Absolute Molar Mass and Beyond: Essential Polymer Characterization with Multi-Angle Light Scattering

    Presented by Dr. Jeffrey Ahlgren, Senior Applications Scientist
    Monday, March 13, 2017

    Characterization techniques based on multi-angle light scattering with size-exclusion chromatography (SEC-MALS) address many of the key analytical challenges in the modern polymer laboratory, including the absolute determination of molecular weight distributions and moments, branching ratios and copolymer composition/molecular weight distributions. This seminar will review light scattering technology, instrumentation and applications to polymer research and development. Select examples will illustrate how SEC-MALS overcomes the limitations of single-detector GPC, facilitating rapid and effective characterization of a wide variety of polymers including polyacrylamides, acrylic copolymers, nylons, styrenes and more. Advanced applications include high-molar-mass branched polymers and fluorescent polymers. Case studies will be presented to illustrate failures of standard SEC-MALS and their solutions via field-flow fractionation (FFF-MALS) and long-wavelength MALS with fluorescence blocking filters. The supplemental roles of Dynamic Light Scattering (DLS) and Viscometry will also be explored.

    Exhibitor RibbonVisit our Booth!

    Place: Michael's at Shoreline Mountain View, CA
    For more info and to Register: Golden Gate Polymer Forum Symposium on Polymer Characterization


    ACS 2017

    Advances in Asymmetric Flow Field Flow Fractionation (AF4) combined with MALS in the characterization of polymers in solution

    Presented by Dr. Jeffrey Ahlgren, Senior Applications Scientist
    Monday, April 3, 2017 | Room 120, Moscone Center | 1:30 PM | Industrial Innovations in Polymer Chemistry Symposium

    Applications in Asymmetric Flow Field Flow Fractionation (AF4) have been growing in number since its commercialization approximately 15 years ago. When coupled with online detectors such as multi-angle liight scattering detectors (MALS) and PALS (phase analysis light scattering detectors), molar mass distributions, size distributions, and even charge distributions can now be measured. AF4 can help confirm results determined by classical techniques such as SEC-MALS, and in cases of heavily branched material that becomes entangled in SEC packing material, fractionation by AF4 can result in improved size distribution analysis due to the nature of the fractionation method. Innovations such as hollow fibers have been developed to reduce sample and solvent requirements without loss of signal sensitivity. For sample types prone to entanglement or significant aggregation resulting from sample concentration during focusing steps, specialized channels such as the frit inlet channel have been developed to permit fractionation of these samples without a focusing step that typically is used during FFF methods. Examples of samples which do not fractionate well by SEC but were successfully fractionationated by FFF will be presented.

    Exhibitor RibbonVisit us at Booth #605

    Place: Moscone Convention Center, San Francisco, CA
    Learn More and Register Here


    ACS 2017

    Benefits of MALS, FFF and Viscometry in the characterization of polysaccharide molar mass, size, conformation and branching ratios

    Presented by Dr. Jeffrey Ahlgren, Senior Applications Scientist
    Thursday, April 6, 2017 | Room 130, Moscone Center | 9:20 AM | Frontiers in Glycoanalytics Symposium

    Complex carbohydrates such as cellulose can be characterized for molar mass, size, conformation and branching following fractionation by Asymmetric Flow Field Flow Fractionation (AF4) or Size Exclusion Chromatography (SEC) using multi-angle light scattering (MALS) and viscometry to measure these important physical characteristics. A multi-detector approach is beneficial for determining more than just molar mass distribution; characteristics such as intrinsic viscosity distributions and determination of conformation and branching characteristics can also be made. Examples of this type of multi-detector analysis will be presented to demonstrate the range and sensitivity that modern instrumentation is now capable of achieving with polysaccharides in solution when fractionated by SEC or FFF methods, including the use of hollow fiber technology with AF4. Recent improvements in on-line viscometry will also be discussed that enhance the sensitivity of detection and software improvements in data analysis to reduce issues from peak broadening and pump pulsations.

    Exhibitor RibbonVisit us at Booth #605

    Place: Moscone Convention Center, San Francisco, CA
    Learn More and Register Here


  • Please check back for upcoming European seminars!


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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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