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Wyatt Technology

Multi-Angle Light Scattering (MALS)

  • DAWN HELEOS II

    DAWN HELEOS II

    The world's most advanced MALS instrument for characterization of macromolecules and nanoparticles in solution to determine absolute molar mass and size.

    In line with HPLC for SEC-MALS, downstream of Eclipse field-flow fractionation, stand alone or with a Calypso, the DAWN HELEOS II offers the highest sensitivity and widest range of measurements for peptides, proteins, conjugates, polymers, and nanoparticles. Optional configurations include temperature control, embedded dynamic light scattering and automated flow-cell cleaning.

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  • miniDAWN TREOS

    miniDAWN TREOS

    Absolute macromolecular analysis of molar mass and size in solution, when the advanced features of the DAWN are not needed.

    The miniDAWN TREOS offers excellent performance over a wide range of molar mass, size and concentration, for characterization of macromolecules and particles below 50 nm in radius.

    Use in line with HPLC for SEC-MALS, downstream of Eclipse field-flow fractionation, with a Calypso for interactions or stand alone. Optional configurations include embedded dynamic light scattering and automated flow-cell cleaning.

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  • µDAWN

    µDAWN

    The first SEC-MALS detector for UHPLC. Uncompromised sensitivity.

    The µDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of proteins, peptides, polymers or nanoparticles below 50 nm in radius directly, without resorting to column calibration or reference standards.

    The µDAWN brings all the advantages of SEC-MALS to the world of UHPLC, including optional embedded dynamic light scattering. Use in conjunction with the Optilab UT-rEX differential refractometer for UHPLC.

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Feature Comparison

Capabilities DAWN HELEOS II miniDAWN TREOS µDAWN
   
Measurements    
Molar Mass Range 200 Da to 1 GDa 200 Da to 10 MDa 200 Da to 10 MDa
Molecular Size Range
(rg)
10 – 500 nm, up to 1000 nm with shape-specific models 10 – 50 nm, up to 150 nm
with shape-specific models
10 – 50 nm, up to 150 nm
with shape-specific models
Molecular Size Range
(Rh, with DLS)
Flow: 1 – 300 nm
Batch: 0.5 nm – 1 µm
Flow: 1 – 40 nm
Batch: 0.5 nm – 1 µm
Flow: 1 - 30 nm
Batch: N/A
MALS Sensitivity 0.4 µg/mL BSA; 0.2 µg BSA typical HPLC loading 1.0 µg/mL BSA; 0.5 µg BSA typical HPLC loading 1.0 µg/mL BSA; 70 ng BSA typical loading on UHPLC-SEC 15 cm column
   
Optics      
Static Scattering Detectors 18 angles 3 angles 3 angles
Laser Power Control Programmable 10% - 100% Programmable 10% - 100% Programmable 10% - 100%
DLS Scattering Angle (optional WyattQELS) any one of 18 static scattering angles 90° 130°
   
Temperature Options Ambient
Ultra-High to +210°C
Heated/Cooled -15°C to +150°C
Ambient only Ambient only

 

See Accessories for MALS

 

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

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ISO Certification

iso-certification

  ISO 9001:2008

   

 

Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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