Wyatt Technology

Conventional size exclusion chromatography relies on reference standards that do not accurately represent your molecules. SEC-MALS measures molar mass directly, independent of elution time, for confidence in reported values.

SEC-MALS

Not all macromolecules behave alike. Your samples may have a different conformation than molecular standards or exhibit unexpected column interactions. As an absolute technique for determining molar mass and rms radius rg in solution, SEC-MALS - the combination of Size Exclusion Chromatography with Multi-Angle Light Scattering analysis - overcomes the limitations of column calibration.

Standard in protein, biopolymer and synthetic polymer characterizations labs around the world, Wyatt MALS detectors are valued for reliable and robust measurements, delivered by our powerful ASTRA software.

Add one or more Wyatt detectors, MALS-certified SEC columns and software to your favorite analytical HPLC system in order to characterize:

  • Heterogeneous biopolymers and synthetic polymers
  • Oligomers and aggregates
  • Conjugates such as ADC PEGylated proteins or block co-polymers

SEC Beam

Multi-angle static light scattering (MALS) requires precise alignment of the beam direction, laser polarization and detector position in order to provide accurate measurements.

Molar Mass

Multi-angle static light scattering (MALS) requires precise alignment of the beam direction, laser polarization and detector position in order to provide accurate measurements.

 

Selected references

  Folta-Stogniew, E. Oligomeric states of proteins determined by size-exclusion chromatography coupled with light scattering, absorbance, and refractive index detectors. Methods Mol. Biol.  2006, 328, 97-112.

  Laurén, J.; Gimbel, D. A.; Nygaard, H. B.; Gilbert, J. W.; Strittmatter, S. M. Cellular prion protein mediates impairment of synaptic plasticity by amyloid-β oligomers. Nature  2008, 457, 1128-1132.

  Ren, X.; Kloer, D. P.; Kim, Y. C.; Ghirlando, R.; Saidi, L. F.; Hummer, G.; Hurley, J. H. Hybrid structural model of the complete human ESCRT-0 complex. Structure  2009, 17, 406-416.

  Tarazona, M. P; Saiz, E. Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of macromolecules. J. Biochem. Bioph. Meth.  2003, 56, 95-116.

  Zhang, M.; Zhang, L.; Cheung, P. C. K.; Dong, J. Fractionation and characterization of a polysaccharide from the sclerotia of Pleurotus tuber-regium by preparative size-exclusion chromatography. J. Biochem. Bioph. Meth.  2003, 56, 281-289.

 BIBLIOGRAPHIC SEARCH

Application notes

Instrumentation for SEC-MALS

  • MALS Detectors

    dawn-heleos-II, sec-mals, size exclusion chromatography, protein aggregation, polymer analysis, polymer molecular weight

    DAWN HELEOS II - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.

    • Heated/cooled option: -15°C to +150°C
    • High-temperature option: to +210°C

    miniDAWN TREOS - Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.

    µDAWN - The first MALS detector for UHPLC, with interdetector dispersion as low as 2 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.

  • Dynamic Light Scattering Detectors

    WyattQELS, instrument accessory, sec-mals, size exclusion chromatography, protein aggregation, polymer analysis, polymer molecular weight

    WyattQELS - A dynamic light scattering (DLS) module which integrates into a DAWN or miniDAWN MALS detector to provide simultaneous DLS measurements in the same MALS scattering volume.

  • Refractive Index Detectors

    optilab-t-rex, sec-mals, size exclusion chromatography, protein aggregation, polymer analysis, polymer molecular weight

    Optilab T-rEX - A unique on-line differential refractometer for measuring concentration of any macromolecule, regardless of chromophores.

    Optilab UT-rEX - The first differential refractometer for UHPLC. For measuring concentration of any macromolecule, regardless of chromophores.


    Viscometer

    ViscoStar II - A highly sensitive, on-line differential viscometer used in conjunction with MALS to determine the size and conformation of all types of biopolymers and synthetic polymers.

  • Accessories

    WyattCOMET, sec-mals, size exclusion chromatography, protein aggregation, polymer analysis, polymer molecular weight

    COMET - An ultrasonic transducer that cleans MALS flow cells automatically by agitating the fluid and loosening particles from the glass surfaces. This module may be integrated into a DAWN, miniDAWN or µDAWN.

    Orbit - The Orbit helps conserve mobile phase by programmatically directing the solvent to either a waste bottle or back to the solvent reservoir.

    RI Calibration Kit - This set of refractive index calibration standards can be used to calibrate any Optilab or third-party differential RI instrument.

    SEC Columns - Wyatt offers silica-based columns specifically designed and tested for optimal SEC-MALS measurements of proteins and peptides.

    Solaris - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination.

    WISH Injection Module - The Wyatt Injection System for High-Pressure (WISH) simplifies manual loading of SEC columns as well as "batch" determination of dn/dc values using an Optilab.

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

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ISO Certification

iso-certification

  ISO 9001:2008

   

 

Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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