Absolute Characterization of Glycoproteins and their Interactions with Proteins and Antibodies by Light Scattering
Join us for a webinar on September 6 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
Glycoproteins (GPs) are primary targets of neutralizing and therapeutic antibodies for a large variety of indications, including epilepsy and many types of viruses. Many non-IgG therapeutic proteins are themselves GPs. Hence, the characterization of GPs and potential degradants, as well as their interactions with therapeutic or target proteins, is essential for understanding GP targets and developing effective therapeutics. This webinar presents two first-principles techniques for carrying out these analyses in solution: SEC-MALS-UV-RI and CG-MALS.
Unlike common unmodified proteins that are globular in conformation and highly hydrophilic in surface chemistry, GPs cannot be characterized accurately by standard analytical size-exclusion chromatography (SEC). Coupling SEC to multi-angle light scattering (MALS) and differential refractive index (dRI) detection results in a powerful SEC-MALS-UV-RI triple-detection system that can determine the molar mass of the protein and total glycan mass in order to characterize degradants and complexes. Composition-gradient multi-angle light scattering (CG-MALS) utilizes the same detectors, with a stop-flow system to quantify the absolute stoichiometry and binding affinity of reversible association between GPs and antibodies or solubilized target proteins.
Characterization of Polymeric Excipients with Size Exclusion Chromatography and Light Scattering
Join us for a webinar on September 21 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
Polymeric and other content can contribute up to 90% of the total mass in a pharmaceutical product, where excipients play a critical role in the drug’s performance. In just one example of the importance of excipient properties for therapeutic effect, the molar mass and degree of branching in polymeric excipients directly impact the rate of drug release.
In this webinar we show how conventional characterization techniques including size exclusion chromatography (SEC) and bulk viscosity measurements may fall short. The addition of light scattering analysis provides complete characterization of molar mass, viscosity, branching, conformation, conjugation, and size of excipients and other polymers.
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Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:
- Essential Polymer Characterization with MALS and FFF
- Small, but bright: µSEC-MALS adds light scattering to UHP-SEC for biophysical characterization of biotherapeutics
- Characterizing Molecular Structure in Synthetic and Natural Polymers by Multi-Angle Light Scattering
- Innovations and Applications of UHP-SEC and Multi-Angle Light Scattering (MALS) for the Analysis of Biotherapeutic Proteins
- Advances in online viscometry: extending the limits of detection for intrinsic viscosity and hydrodynamic radius
- Biotherapeutics Form and Function: Case Studies in Light Scattering
- Characterizing Microgel Polymers and Nanostructures by Light Scattering
- Optimizing Protein Biotherapeutic Formulations with the Light Scattering Toolkit
- Measuring Size, Stability and Conformation of Biopolymers
- Drug Delivery Nanoparticles Illuminated: the Light Scattering Toolkit-Part 2, Online Measurements
- Techniques de diffusion de lumière pour l’étude et la caractérisation de composés biothérapeutiques
- Molmassen mit weniger Probe schneller und besser messen – mit dem neuen µDAWN Lichtstreudetektor für die UHPLC
- Verbessern Sie Ihre Proteinanalytik durch den Einsatz von Streulichtdetektoren
- Asymmetric Flow Field Flow Fractionation (AF4): A Powerful Alternative to SEC