Wyatt Technology

Field-Flow Fractionation Combined with Multi-Angle Light Scattering

 

Presented Live: June 11, 2009

Field-Flow Fractionation (FFF) is a technique that fractionates macromolecular species using laminar flow without any stationary phase present. The advantage of this technique is that it can fractionate a broad range of sizes in a single run; by removing the stationary phase from the method, problems with sample absorption are frequently eliminated. When this technique is combined with downstream detection by multi-angle light scattering (MALS) and a concentration detector, the molar mass and size can be determined and true polydispersity of the sample can be evaluated. This webinar explains how the principle of FFF works and presents a wide variety of applications for FFF-MALS analysis.

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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