Wyatt Technology

Innovations and Applications of UHP-SEC and Multi-Angle Light Scattering (MALS) for the Analysis of Biotherapeutic Proteins

 

Presented Live: October 25, 2016

Speakers:
Dr. Stephan Koza, Principal Applications Chemist, Waters Corp.
Dr. John Champagne, Senior Applications Scientist, Wyatt Technology Corp.

Ultra-high performance size exclusion chromatography (UHP-SEC) is gaining in popularity owing to the high speed and resolution of separation compared to standard HP-SEC. Online multi-angle light scattering (MALS) is the recognized method for obtaining absolute molecular weight and size, independently of column retention time, making it indispensable for accurate and reliable characterization by SEC. However, light scattering detectors designed for HPLC-SEC are in general not suitable for UHP-SEC.

In this webinar we discuss the practical application of UHP-SEC with regards to method development and successful implementation. This will be followed by a discussion on limitations of standard MALS detectors and present the capabilities of a MALS detector designed explicitly to meet the challenges of UHP-SEC. The utility of UHP-SEC combined with MALS–dubbed ‘µSEC-MALS’–as well as online dynamic light scattering will be demonstrated in the context of protein characterization.

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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