The drive to develop synthetic rubber during World War II spurred the first research efforts into theory and practice of light scattering for characterization of macromolecules in solution. Since then light scattering technology has evolved into the sophisticated, robust instruments developed – and continually refined - by Wyatt Technology. Absolute analysis of synthetic polymers to determine molecular weight, size, branching and conformation remains one of the primary applications of multi-angle light scattering.
Sample and reference polymers with the same hydrodynamic volume elute from a GPC column at the same time, but do not have the same molecular weights. SEC-MALS determines polymer molar mass independently of elution time.
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