Wyatt Technology
  • What is ECLIPSE?

    The ECLIPSE software controls the Eclipse DualTec and Eclipse AF4 Field-Flow Fractionation (FFF) systems. Coupled with ASTRA for data collection and analysis, ECLIPSE provides users with a turn-key solution for macromolecular characterization.

    The ECLIPSE software uses either Agilent, Dionex, or Shimadzu chromatography components for flow delivery and sample injection. Users can stay with their HPLC software platform and run their Eclipse instrument with the familiar interface they already know. Agilent users will work within the ChemStation software environment for complete system control, and Shimadzu owners use software that interfaces with LC Solutions for Eclipse system control.

    The ECLIPSE software provides a control center for starting an experiment, either for a single run or for an unattended sequence of runs. Once a separation has been completed, data processing takes place in ASTRA, the powerful and versatile software for MALS data. ASTRA features many special and proprietary algorithms, including band broadening correction, copolymer and protein conjugate analysis, number density calculation, and distribution analysis. The result is a seamless system for high resolution separation and cutting-edge detection technology.

  • ECLIPSE Control 1

    The job of controlling HPLC instruments is best done by the original vendor's own HPLC software. Therefore, the Eclipse does not use its own controller software, but integrates into various HPLC packages. There are many benefits to this approach. Users can stay within their HPLC software platform and run the Eclipse with the familiar interface they already know. The complete portfolio of HPLC equipment can be integrated with all the sophisticated features and options that the HPLC manufacturer offers. Existing HPLC instruments can be integrated and local support and service from the original supplier is available. If the HPLC components require maintenance, the local service organization of the HPLC vendor takes care of this task.

    Currently the Eclipse operates with Agilent, Dionex, and Shimadzu HPLCs. For owners of Agilent HPLC systems, a ChemStation or OpenLAB add-in module is installed which creates access to the Eclipse as an instrument in ChemStation. ChemStation data files can store specific data traces, like pressure reading, cross flow and inject flow rates, etc. A similar integration exists for the LCsolution software from Shimadzu. Additional HPLC software packages will be integrated in the near future.

    Manual control of all flow modes and flow rates is controlled by easy access to software menus that permit system preparation and equilibration prior to sample runs.

    ECLIPSE Control 2


    The Eclipse instrument is configured using the standard ChemStation Hardware Configuration dialog interface:

    ECLIPSE Control 3


    Eclipse instrument settings can be viewed from within ChemStation for convenience:

    ECLIPSE Control 4

  • AF4 separation methods are generated and stored in ChemStation through the interactive method design window that simplifies the process of fractionating a wide variety of sample applications.

    Data Collection


    Cross flow gradients are easy to program using the Cross Flow Assistant. Cross flow fields can be constant, linear decreasing cross flow or exponential decreasing cross flow in order to resolve complex sample compositions.

    Method Development

  • The flexibility of AF4 allows one to achieve optimal separation for most applications, but in some cases it can be difficult to find the optimal conditions for the separation run. Therefore, Wyatt Technology provides you with an indispensable tool for the method development by simulating an AF4 separation. All relevant parameters are entered by the user, and the resulting separation is immediately shown, including the effect of band broadening and dilution of the sample at the channel outlet.

    Helpful Tools 1

    Helpful Tools 2


    The example below shows a simulated BSA separation comparing two experimental conditions:

    • The green experiment uses a 350 µm spacer, constant cross flow at 3 mL/min, and a SC (153 mm length) channel, compared to the red experiment, which uses a 490 µm spacer and a gradient cross flow from 2 – 0 mL/min in 20 minutes in the MC (114 mm length) channel.

    • The red experiment has higher resolution, the peaks are narrower and solvent consumption is reduced.

    Without the Optimizer software it would have been impossible to predict the result of varying several parameters at the same time. The Eclipse Optimizer provides a vital shortcut to find the best separation conditions.

    Helpful Tools 3

  • Host Computer Requirements

    Please see Host Computer Requirements for details of the computer hardware required by ECLIPSE.


    Operating System

    Windows 7 Compatibility

    • Microsoft Windows 7/8.1 Professional/Ultimate (32- and 64-bit): Agilent OpenLAB
    • Microsoft Windows 7/8.1 Professional/Ultimate (32-bit): Agilent ChemStation, Dionex Chromeleon, Shimadzu LabSolutions
    • Microsoft Windows XP Professional (32-bit): Agilent ChemStation, Agilent OpenLAB, Dionex Chromeleon, Shimadzu LCsolution, Shimadzu LabSolutions

    For Agilent ChemStation, Agilent OpenLAB, Dionex Chromeleon and Shimadzu LCsolution software requirements, please visit the respective vendor websites.


   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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