Wyatt Technology

DynaPro Plate Reader II

DynaPro Plate Reader II

 

High-throughput, automated, temperature-controlled Dynamic Light Scattering.

Revolutionize your performance by replacing your conventional, cuvette-based dynamic light scattering unit with the automated DynaPro® Plate Reader II. The DynaPro excels at high-throughput sizing and screening of pharmaceutical and nanoparticle formulations to enable novel workflows that simply would not be possible with other DLS instruments. Even if you need to run just a handful of samples, it's a great productivity booster.

 Watch a video to learn more.

 View this video to see how easy it is to make measurements with the DynaPro Plate Reader II, courtesy of Yale University Chemical and Biophysical Instrumentation Center.

Included with each DynaPro Plate Reader is a copy of DYNAMICS, our powerful software package for analyzing automated batch dynamic light scattering. The Security Pack (DYNAMICS SP) option provides 21 CFR (11) compliance for GMP/GLP use.

  • Revolutionize DLS

    The DynaPro Plate Reader II offers dynamic light scattering (DLS) measurements of the size and interactions of proteins, nanoparticles and other macromolecules in situ in industry-standard microwell plates. No liquid handling occurs after dispensing into the plates, to maximize throughput and minimize sample carryover. This ground-breaking instrument provides rapid optimization of the formulation process for:

    Key features

    • Measure hydrodynamic radius from 0.5 nm to 1000 nm
    • Sensitivity down to 0.125 mg/mL lysozyme
    • Industry standard microwell plates of 96, 384 or 1536 wells
    • All measurements made in situ in the wells
    • Temperature ramps from 4°C to 85°C
    • Integrates with plate-based liquid handling robotics

    A wealth of applications

    The DynaPro Plate Reader II provides unparalleled levels of ease-of-use, productivity, reproducibility, and flexibility in order to address additional demanding applications such as:

    • Optimizing protein crystallization
    • Assessing aggregation or oligomerization
    • Determining protein stability-indicating parameters such as thermal denaturation and colloidal stability
    • Screening high-concentration protein solutions for reversible association and viscosity

    • A wealth of features

      • Rapid or slow temperature ramps 4°C - 85°C with excellent uniformity across the plates. Samples are heated in parallel and all filled wells measured at each temperature
      • Well read times as short as 5-10 seconds
      • Integration with liquid-handling robots for higher levels of automation, especially when your workflow is plate-based, and utilizes spectroscopic and other plate readers in addition to DLS
      • A camera for viewing each well from below, to aid in data diagnostics (e.g. to detect precipitation or bubble formation)
    • A set of images collected by the onboard camera, showing clean wells and wells containing bubbles, crystals and precipitates.

    • Lab automation

      An OLE software API is available for interfacing the DynaPro Plate Reader II to robotic liquid handling in the high-throughput lab. This capability allows for fully automated plate loading and data collection.

      Wyatt partners with several companies that offer integrated solutions for automated loading and analysis of plated samples: 


    • Freeslate Biologics Formulation System
      Freeslate Biologics Formulation System

      Click to enlarge

        Contact Freeslate


    • Hudson Robotics Plate Transfer

      Click to play video

        Contact Hudson Robotics

    • In addition, Hamilton Robotics, Caliper Life Sciences, and Tecan offer custom integration services.

  • DYNAMICS 7, software

    DYNAMICS - Automates well-plate scans, temperature ramps, etc. and analyzes DLS data to determine size distributions, temperature onset of melting or aggregation, and the diffusion interaction parameter kD.

  • Supported Well Plate Formats 96, 384 or 1536 *
    Size Range (Radius, Rh) 0.5 to 1000 nm
    Minimum Concentration @ 14 kDa 0.125 mg/mL
    Optics
       Laser Wavelength 830 nm
       Laser Power Control Programmable 10% – 100%
       Attenuation Range 1 to 106
    Temperature Control 4 – 85°C ††
    Fluidics
       Minimum Sample Volume 4 µL †††
    Data Acquisition Time 1 to 3600 seconds per well per read
    Read Time per Well 5 – 20 seconds
    Electronics
       Onboard Camera 3 megapixels ††††
       Digital Communication Ethernet (TCP/IP)
    Dimensions 60 cm (L) x 36 cm (W) x 25 cm (H)

    * Supports many industry standard well plates. Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. for a list of supported plates.

    Minimum concentration specified with 50 µL of Lysozyme (14 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

    †† Accuracy of ± 0.5°C for 4 – 50°C, and ± 1°C for 50 – 80°C. Minimum temperature of 4°C requires a laboratory temperature of 24°C or below.

    ††† Minimum volume specified with 2 mg/mL Lysozyme in a 1536 well plate, Wyatt P/N P8803-1536, with 25 seconds acquisition.

    †††† Camera operates with sample temperature in the range of 4 – 50°C, and automatically powers off above 50°C.


    Host PC requirements may be found in Computer Requirements.

    Specifications subject to change without notice.

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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