Share
Wyatt Technology

miniDAWN TREOS

miniDAWN TREOS

 

The Multi-Angle Light Scattering Detector for Absolute Macromolecular Analysis.

The miniDAWN TREOS Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering superb sensitivity over a wide range of molecular weight, size and concentration. TREOS performance for MALS analysis is exceeded only by the DAWN HELEOS II which covers a wider range of sizes and molecular weights with even better sensitivity.

Included with each miniDAWN is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.

  • The miniDAWN TREOS is most often used in conjunction with Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition, independent of column calibration by reference standards.

    The miniDAWN and its companion Optilab T-rEX dRI detector are well-established for MALS analysis, cited in thousands of peer-reviewed publication. With the addition of a ViscoStar II differential viscometer or WyattQELS module, the triple-detection system can analyze conformation and sizes below the rg limit.

    • Mw from 200 Da to 10 MDa
    • Sensitivity: 1.0 µg/mL BSA (66.4 kDa)
    • rg from 10 to 50 nm, up to 150 nm with shape-specific models
    • Ultra-stable laser

    The TREOS may also be used in batch (off-line) mode or in conjunction with a Calypso II CG-MALS system for characterization of macromolecular interactions: affinity and absolute stoichiometry, label-free and immobilization-free, as well as second virial coefficients, cross virial coefficients, average molar masses and sizes.

    The TREOS is only available as an ambient-temperature detector.

  • Dynamic Light Scattering

    WyattQELS - A dynamic light scattering (DLS) detection module which integrates inside the miniDAWN to provide simultaneous DLS measurements in the same scattering volume. The optical collection fiber is held in a dedicated 90° fiber mount.

    DynaPro NanoStar - Primarily used for batch DLS, the NanoStar does double duty as an online DLS detector by installing its optical collection fiber into the miniDAWN.


    Ultrasonic Cleaning

    COMET - An ultrasonic transducer that cleans MALS flow cells automatically by agitating the fluid and loosening particles from the glass surfaces. Integrates inside the miniDAWN.

  • The miniDAWN may be positioned downstream of all types of standard GPC, HPLC or FPLC systems for analysis of molar mass and size distributions (see SEC-MALS page), and derivatives such as conformational analysis. For those systems equipped with a UV, RI, fluorescence or other type of concentration detector, the miniDAWN accepts up to 4 analog inputs so a 3rd-party concentration signal may be used in MALS calculations of molar mass. The miniDAWN is generally set up downstream of a UV detector and upstream of a RI detector.

    In most cases it is beneficial to connect an Optilab T-rEX as well as a UV detector. This permits universal concentration detection regardless of fluorophores or chromaphores. The combination of UV and RI signals is utilized in ASTRA's Conjugation Analysis algorithm to characterize binary complexes such as glycoproteins, PEGylated proteins, membrane proteins enveloped in surfactant, or block co-polymers.

    The miniDAWN may also be connected to an Eclipse Field-Flow Fractionation system for characterization of macromolecules and nanoparticles from 1 nm to > 100 nm via FFF-MALS.

    Both SEC-MALS and FFF-MALS will benefit from an additional WyattQELS module for measuring nanoparticle size via dynamic light scattering. The WyattQELS utilizes the same laser and flow cell volume as MALS detection. Its collection fiber, placed at 90° to the laser beam, does not displace any of the three detectors in the miniDAWN.

    For analysis of natural and synthetic polymers, the ViscoStar II may also be added as well. Differential viscometry is an orthogonal means of determining molar mass and conformation.

  • MALS detectors may be operated in batch (unfractionated) mode when the analysis does not benefit from separation:

    • If the sample is not amenable to separation by SEC or FFF, to measure weight-average molar mass, and size as z-average radius
    • In order to validate SEC-MALS measurements:
      • to determine if large aggregates or other species are filtered out on the column
      • to determine if reversible aggregates dissociate subsequent to dilution on the column or FFF separation channel
    • In order to characterize macromolecular or nanoparticle complexes which are disrupted by column shear or dilution
    • In order to measure binding affinity or reaction kinetics of macromolecules such as protein-ligand interactions

    Calypso II - Automated sample preparation and delivery system for carrying out and analyzing composition-gradient MALS in a miniDAWN. Analyses include biomolecular interactions analyses high-concentration protein-protein interactions, Zimm plots and dn/dc as well as time-resolved MALS. Utilizes the CALYPSO software for interaction analysis.

    microCUVETTE - Allows static and dynamic light scattering measurements to be made simultaneously in a MALS detector using a quartz cuvette, requiring only 10 µL.

    NanoFilter Kit - Makes it easy to filter, recover and refilter precious sample. This is especially useful when your total sample volumes are below 300 µL.

    Microbatch Kit - Used to inject samples into the flow cell of a MALS detector, and in determining dn/dc with an RI detector.

  • Orbit - The Orbit helps conserve mobile phase by programmatically directing the solvent to either a waste bottle or back to the solvent reservoir. In a CG-MALS system, the Orbit is used to automatically select wash solvents in the post-experiment cleaning cycle.

    SEC Columns - Wyatt offers silica-based columns specifically designed and tested for optimal SEC-MALS measurements of proteins and peptides.

    Solaris - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination.

    WISH - The Wyatt Injection System High-Pressure (WISH) triggers ASTRA data collection and is ideal for use in SEC-MALS without an autoinjector. Also used with an Optilab dRI detector for the determination of dn/dc values.

    SysTrack - Convenient shelving for holding multiple detectors in your experimental setup.

    Thermos - A temperature-controlled chamber for SEC/GPC columns and FFF channels.

  • ASTRA - Our comprehensive software solution for MALS and DLS analysis in chromatography, AF4 or batch mode, included with each detector. ASTRA is available in a 21 CFR(11) compliant version and offers additional options such as particle analysis and a research database.

    CALYPSO - Software for automated composition-gradient MALS experiments: control of the Calypso II, data acquisition and analysis for biomolecular interactions and reaction kinetics as well as weight-average molar mass, z-average molecular size, and virial coefficients.

  • Measurements  
       Molar Mass Range 200 Da to 10 MDa *
       Molecular Size Range (rg) 10 nm to 50 nm , up to 150 nm with shape-specific models *
       Molecular Size Range (Rh, with    WyattQELS DLS module or
       DynaPro NanoStar)
    In flow mode, 0.5 nm – 40 nm; in batch mode, 0.5 nm – 1 µm *††
       Sensitivity 1.0 µg/mL BSA; 0.5 µg BSA typical HPLC loading
    Fluidics  
       Mobile Phase Compatibility All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.
    Optics  
    Detectors  
       MALS Detectors High-gain, high dynamic range, static light scattering detectors at 3 angles
       Auxiliary Detectors Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.
       A/D Resolution 24 bit (detector dynamic range > 16,000,000)
    DLS Detector (optional) WyattQELS dynamic light scattering module installs directly inside the miniDAWN chassis.

    Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument may be installed in the miniDAWN.
    Laser Properties  
       Laser Wavelength 658 nm (nominal). Each instrument is individually characterized.
       Laser Power Control Programmable 10% - 100%
    Flow Cells Fused Silica, optimal for solvent refractive index < 1.50
    F2, optimal for solvent refractive index > 1.50
    Electronics  
       Analog Inputs 4 differential analog inputs with 24 bit resolution
       Analog Outputs 3 single ended analog outputs -10 V to +10 V
       Other Inputs/Outputs Alarm In, Alarm retransmit, Auto-Inject in, Autoinject contact closure, Recycle In, Recycle valve power
       Computer Interface Ethernet
       Processed Data Transmission Rate Software selectable from 36.6 Hz to 0.2 Hz
       Front Panel Display 162.5 mm, 16-bit, high resolution touch screen
    Dimensions 60 cm (L) x 36 cm (W) x 17 cm (H)

    * Typical. Actual range depends on dn/dc, sample concentration, sample conformation and chromatography conditions.

    Typical. Actual range depends on the sample.

    †† Assuming a flow of 0.3 mL/min.


    Facilities requirements may be found in Wyatt Instrument Facilities Specifications.
    Host PC requirements may be found in Computer Requirements.

    Specifications subject to change without notice.

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

13 Key Questions to Ask

ultimate-guide

Get the inside scoop on buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument!

Request the Ultimate Guide today.
Get Ultimate Guide

 

ISO Certification

iso-certification

  ISO 9001:2008

   

 

Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



© Wyatt Technology Corporation. All rights reserved.