The miniDAWN may be positioned downstream of all types of standard GPC, HPLC or FPLC systems for analysis of molar mass and size distributions (see SEC-MALS page), and derivatives such as conformational analysis. For those systems equipped with a UV, RI, fluorescence or other type of concentration detector, the miniDAWN accepts up to 4 analog inputs so a 3rd-party concentration signal may be used in MALS calculations of molar mass. The miniDAWN is generally set up downstream of a UV detector and upstream of a RI detector.
In most cases it is beneficial to connect an Optilab T-rEX as well as a UV detector. This permits universal concentration detection regardless of fluorophores or chromaphores. The combination of UV and RI signals is utilized in ASTRA's Conjugation Analysis algorithm to characterize binary complexes such as glycoproteins, PEGylated proteins, membrane proteins enveloped in surfactant, or block co-polymers.
The miniDAWN may also be connected to an Eclipse Field-Flow Fractionation system for characterization of macromolecules and nanoparticles from 1 nm to > 100 nm via FFF-MALS.
Both SEC-MALS and FFF-MALS will benefit from an additional WyattQELS module for measuring nanoparticle size via dynamic light scattering. The WyattQELS utilizes the same laser and flow cell volume as MALS detection. Its collection fiber, placed at 90° to the laser beam, does not displace any of the three detectors in the miniDAWN.
For analysis of natural and synthetic polymers, the ViscoStar II may also be added as well. Differential viscometry is an orthogonal means of determining molar mass and conformation.