Multi-Angle Light Scattering (MALS) Detectors

DAWN®

The world's most advanced MALS instrument for characterization of macromolecules and nanoparticles in solution to determine absolute molar mass and size.

In line with HPLC for SEC-MALS, downstream of Eclipse field-flow fractionation, stand alone or with a Calypso, the DAWN offers the highest sensitivity and widest range of measurements for peptides, proteins, conjugates, polymers, and nanoparticles. Optional configurations include temperature control, embedded dynamic light scattering and automated flow-cell cleaning.

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miniDAWN®

The best in basic multi-angle light scattering for absolute macromolecular analysis

The miniDAWN determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to SEC/GPC, it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.

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microDAWN™

The first SEC-MALS detector for UHPLC. Uncompromised sensitivity.

The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of proteins, peptides, polymers or nanoparticles below 50 nm in radius directly, without resorting to column calibration or reference standards.

The microDAWN brings all the advantages of SEC-MALS to the world of UHPLC, including optional embedded dynamic light scattering. Use in conjunction with the Optilab differential refractometer for UHPLC.

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ultraDAWN™

A breakthrough in process analytical technology for production of nanoparticles, biopharmaceuticals and polymers.

For the first time, critical quality attributes, or CQAs – including weight-average molecular weight Mw and rms radius Rg – can be monitored directly, in-line or on-line, for rapid feedback on product and process quality.

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Feature Comparison

Capabilities DAWN® miniDAWN® microDAWN™ ultraDAWN™
Environment Analytical HPLC/FPLC Analytical HPLC/FPLC Analytical UHPLC Process
Measurements
Molar Mass Range 200 Da to 1 GDa 200 Da to 10 MDa 200 Da to 10 MDa 1 kDa to 1 GDa
Molecular Size Range
(Rg)
10 to 500 nm, up to 1000 nm with shape-specific models 10 to 50 nm, up to 150 nm
with shape-specific models
10 to 50 nm, up to 150 nm
with shape-specific models
10 to 250 nm
Molecular Size Range
(Rh, with DLS)
Flow: 0.5 to 300 nm
Batch: 0.5 nm to 1 µm
Flow: 0.5 to 50 nm
Batch: 0.5 nm to 1 µm
Flow: 0.5 to 30 nm
Batch: N/A
N/A
MALS Sensitivity 10 ng of 100 kDa polystyrene in THF1;
200 ng of BSA in PBS2
25 ng of 100 kDa polystyrene in THF1;
500 ng of BSA in PBS2
3.5 ng of 100 kDa polystyrene in THF3;
70 ng of BSA in PBS3
Process Dependent
Optics
Static Scattering Detectors 18 angles 3 angles 3 angles 18 angles
Laser Power Control Programmable 10% to 100% Programmable 10% to 100% Programmable 10% to 100% Programmable 10% to 100%
DLS Scattering Angle
(optional WyattQELS)
any one of 4 dedicated locations 135° 130° N/A
Temperature Options Ambient
Ultra-High to +210°C
Heated/Cooled -15°C to +150°C
Ambient only Ambient only Ambient only
Host Software ASTRA ASTRA ASTRA OBSERVER

1Under HPLC-SEC conditions with 7.8x300 mm columns.
2Under HPLC-SEC conditions with 7.8x300 mm Wyatt SEC protein columns.
3Under UHPLC-SEC conditions with 4.6x150 mm columns.