Multi-Angle Light Scattering (MALS) Detectors
Feature Comparison
Capabilities | DAWN® | miniDAWN® | microDAWN® | ultraDAWN™ |
Environment | Analytical HPLC/FPLC | Analytical HPLC/FPLC | Analytical UHPLC | Process |
Integrated DLS module | Optional WyattQELS | Optional WyattQELS | Optional WyattQELS | No |
DLS fiber connection | Optional, to NanoStar or Mobius | Optional, to NanoStar or Mobius | Optional, to NanoStar or Mobius | Not available |
Measurements | ||||
Molar Mass Range | 200 Da to 1 GDa | 200 Da to 10 MDa | 200 Da to 10 MDa | 1 kDa to 1 GDa |
Molecular Size Range (Rg) |
10 to 500 nm, up to 1000 nm with shape-specific models |
10 to 50 nm, up to 150 nm with shape-specific models |
10 to 50 nm, up to 150 nm with shape-specific models |
10 to 250 nm |
Molecular Size Range (Rh, with DLS) |
Flow: 0.5 to 300 nm Batch: 0.5 nm to 1 µm |
Flow: 0.5 to 50 nm Batch: 0.5 nm to 1 µm |
Flow: 0.5 to 30 nm Batch: N/A |
N/A |
MALS Sensitivity |
10 ng of 100 kDa polystyrene in THF1; 200 ng of BSA in PBS2 |
25 ng of 100 kDa polystyrene in THF1; 500 ng of BSA in PBS2 |
3.5 ng of 100 kDa polystyrene in THF3; 70 ng of BSA in PBS3 |
Process Dependent |
Optics | ||||
Static Scattering Detectors | 18 angles | 3 angles | 3 angles | 18 angles |
Laser Power Control | Programmable 10% to 100% | Programmable 10% to 100% | Programmable 10% to 100% | Programmable 10% to 100% |
DLS Scattering Angle (optional WyattQELS) |
any one of 4 dedicated locations | 135° | 130° | N/A |
Temperature Options |
Ambient Ultra-High to +210 °C Heated/Cooled -15 °C to +150 °C |
Ambient only | Ambient only | Ambient only |
Host Software | ASTRA | ASTRA | ASTRA | OBSERVER |
1Under HPLC-SEC conditions with 7.8x300 mm columns.
2Under HPLC-SEC conditions with 7.8x300 mm Wyatt SEC protein columns.
3Under UHPLC-SEC conditions with 4.6x150 mm columns.