Wyatt Technology


Composition-Gradient, Multi-Angle Static Light Scattering (CG-MALS) is a powerful technique for characterizing a wide range of biomolecular interactions.

CG-MALS

Label-free and immobilization-free, CG-MALS is a rigorous yet versatile first-principles technique that measures interactions directly by monitoring the changes in solution molar mass that arise from the formation or dissociation of complexes. Apply CG-MALS to characterize:

  • Self- and hetero-association
  • Binding affinity from pM to mM
  • Absolute molecular stoichiometry (not just mole ratios)
  • Multi-valent or cooperative interactions; simultaneous self- and hetero-association
  • Kinetics of binding, aggregation or dissociation over time scales of seconds to hours

Self- and Hetero-Association

CG-MALS characterizes self- and hetero-association.

Binding Kinetics
CG-MALS works by mixing different compositions of samples and diluents, then measuring the weight-average molar mass of the solution at each composition step. Binding kinetics are often apparent.

CG-MALS also characterizes non-specific interactions between proteins at high concentrations and between proteins and excipients, which impact colloidal stability of formulated biotherapeutics. This email address is being protected from spambots. You need JavaScript enabled to view it. to learn how CG-MALS can address your specific biomolecular interaction analyses, such as:

  • Drug-target binding for monoclonal antibodies or bispecific fusion proteins
  • Monovalent or multi-valent receptors
  • Self-association under a variety of buffer conditions
  • Multi-protein assemblies in structural biology
  • Colloidal stability at protein concentrations of 100 mg/mL and beyond

Protein Assemblies

CG-MALS excels at analyzing multi-protein assemblies.

Instrumentation for CG-MALS

  • Composition-Gradient System

    Calypso, CG-MALS, biomolecular interactions

    Calypso® - Composition-gradient preparative system + software for carrying out and analyzing CG-MALS interaction analyses. Requires a MALS detector.

  • MALS Detectors

     dawn, molar mass determination, molar mass measurement, molar mass analysis, molecular weight measurement, molar mass 
              characterization

    DAWN® - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.

    • Standard option: ambient temperature
    • Heated/cooled option: -15°C to +150°C
    • High-temperature option: ambient to +210°C

    The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.


    miniDAWN®
    - Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.

    DynaPro® NanoStar® - With sample volumes as small as 1.25 µL and temperature control spanning -15°C to +150°C, the NanoStar goes above and beyond traditional cuvette-based DLS instruments: it offers an optimized static light scattering detector (in parallel to the DLS detection system) which may be used with the CALYPSO software to perform CG-MALS experiments with very little sample. Samples must be prepared manually, without benefit of the Calypso's automation.

  • Dynamic Light Scattering Detector

    WyattQELS, CG-MALS, binding affinity determination, light scattering, protein interactions, equilibrium dissociation coefficient

    The CALYPSO® software does not analyze DLS data, but can be set to trigger DLS data acquisition by Wyatt’s ASTRA® software.

    WyattQELS - A dynamic light scattering (DLS) module which integrates into a DAWN or miniDAWN MALS detector to provide simultaneous DLS measurements in the same scattering volume.

    DynaPro® NanoStar® - With sample volumes as small as 1.25 µL and temperature control spanning -15°C to +150°C, the NanoStar goes above and beyond traditional cuvette-based DLS instruments: it offers an optimized static light scattering detector (in parallel to the DLS detection system) which may be used with the CALYPSO software to perform CG-MALS experiments with very little sample. The NanoStar does double duty as an online DLS detector by installing its optical collection fiber into a Wyatt MALS detector.

  • Refractive Index Detector

    Optilab, CG-MALS, dawn-heleos-II, binding affinity determination, light scattering, protein interactions

    Optilab® - A unique on-line differential refractometer for measuring concentration of any macromolecule, regardless of chromophores. The high-concentration Optilab accommodates protein concentration up to 180 mg/mL.

  • Accessories

    Orbit™ - In a CG-MALS system, the Orbit is used to automatically choose wash solvents in the post-experiment cleaning cycle.

    COMET™ - An ultrasonic transducer that cleans MALS flow cells automatically by agitating the fluid and loosening particles from the glass surfaces. This module is included with each DAWN, miniDAWN and microDAWN.

    microCUVETTE™ - Allows static and/or dynamic light scattering measurements to be made in a quartz cuvette in a DAWN, in order to reduce sample consumption to the bare minimum - requiring only 10 µL per composition step. Samples are prepared manually rather than by means of the Calypso, while the CALYPSO software is still used to acquire and analyze data.

    NanoFilter Kit - Makes it easy to filter, recover and refilter precious sample when the microCUVETTE is used for CG-MALS.

    RI Calibration Kit - Pre-made RI calibration standards are available to speed up RI calibration. Can be used to calibrate any Optilab or third-party differential RI instrument.

    Solaris™ - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination.

  • Software

    CALYPSO, software

    CALYPSO™ - The CALYPSO software orchestrates CG-MALS analysis of biomolecular interactions, controlling sample preparation and delivery, data acquisition and data analysis. Provides the most extensive suite of association models available to determine the affinity and absolute molecular stoichiometry of macromolecular interactions including protein-protein binding, binding of aptamers and peptides or proteins, etc., as well as first-order analysis of reaction kinetics.

   

Ultimate Guide

Thirteen key questions to ask when buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument.

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Solutions Catalog

Our Solutions Catalog combines Wyatt's product lines, applications and services into a single comprehensive booklet.

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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