ASTRA®

Luminary software for macromolecular and nanoparticle separation and characterization
 

ASTRA

Turn your data into results with the ultimate in light scattering software—ASTRA.

The most powerful and versatile software available for the characterization of macromolecules and nanoparticles via multi-angle and dynamic light scattering.

  • Maximize your productivity via ASTRA's One-Click MW™ feature, automated multi-sequence processing and HPLC integration.
  • Take advantage of its integrated control of Agilent systems, or its support for Waters Empower sequences.

ASTRA offers a suite of basic and advanced analyses, including customized reports and an optional 21CFR(11)-compliant security and database package.

It greatly simplifies MALS and DLS analysis, so you can quickly and accurately assess molar mass, size, conformation, conjugation and other essential physical parameters.

With each analysis, ASTRA provides the quantitative experimental uncertainties that transform mere numbers—into science.

ASTRA Features and Capabilities

Browse these tabs to learn about the many capabilities and features in ASTRA which make it is the most powerful and versatile software available for the characterization of macromolecules and nanoparticles via multi-angle and dynamic light scattering.

One-Click MW™

One-Click MW

Don't waste time - from sample to results in one click. Read more...

 

One Click: From Sample to Results

One-click MW

 
ASTRA 7 increases productivity for routine analyses

Running a SEC-MALS analyses has never been easier. ASTRA’s One-Click MW™ feature complete an entire method at the click of a button.

Click on Run Default and ASTRA will:

  • Synchronize data collection with your HPLC
  • Autoset parameters to determine MW and Rg
  • Generate custom reports and graphs
  • Prepare for the next run

run-default-button

Method Builder

ASTRA's Method Builder simplifies the specification of methods for the most common protein and polymer analyses, in three intuitive steps:

  • Choose an analysis profile
  • Specify the instruments
  • Enter additional parameters

Then save the method, load the sample(s), click Run Default and return later to find out the MW results in the final report.

Freedom to Explore

With ASTRA, you are never locked in to default methods or standard procedures. You always* have access to each parameter and processing step to review, validate and improve.

*Except network accounts where access is limited under the 21CFR(11) compliant Security Pack.

Ahead of the Curve

As you build expertise and confidence, and move on to complex analyses, ASTRA allows you to refine the results of automated processing with fine adjustments to peak selection, baseline specification, detector filtering and other settings.

HPLC Control

Full digital synchronization between your HPLC system, Wyatt detectors and ASTRA 7:

  • Minimizes user errors
  • Runs SEC-MALS from a single, familiar environment
  • Saves you time and money

ASTRA 7's HPLC control modules works with standard Agilent hardware including pumps, autosamplers, column ovens and UV/Vis detectors.*

*Control of additional HPLC instruments coming soon

HPLC Manager

Integrated HPLC control

Let ASTRA take control of your HPLC stack for effortless synchronization between injections and data collection. ASTRA 7 eliminates the need for separate HPLC and light scattering software. HPLC modules are listed together with Wyatt detectors in a common experiment configuration, and key parameters such as vial position, injection volume and flow rate specified in ASTRA's sequences.

HPLC Manager

HPLC Manager

The HPLC Manager app controls and monitors HPLC modules including pumps, column ovens, UV detectors and autosamplers, right from the desktop. Included free with the HPLC Control module.

High-Throughput Processing

Built from the ground up to handle with ease large sets of data, ASTRA has the tools you need to process and report on sequences containing many samples, including replicates. "One-to-Many" processing interface lets you focus on your goals, and leave the drudgery to the computer.

EASI Table

Everything you need at a glance.

Quickly compare multiple data runs with the EASI Table feature to assess compound stability and consistency. The table data can be exported in a variety of formats, or copied and pasted into your favorite word processing software.

Specify which data should be displayed in EASI Table, as well as the format and precision used to display the values. Choose from percent or numerical uncertainties, or omit them entirely.

EASI Table

Sequence Processing

Collect data while you sleep.

Sequences are used to specify and control the automated collection of data as well as system functions. A sequence can be configured with the default method or a different method can be specified for each sample. Sequences also perform post-collection maintenance, such as running the COMET flow cell cleaner or switching the Orbit to solvent recycle mode.

Sequence

In the Samples view, it is possible to modify sample parameters on-the-fly, adding samples, changing collection times, or skipping samples.


Let your computer do the work.

ASTRA allows you to process an experiment as you wish, then instruct the computer to apply the same settings and analysis steps on any number of additional data files. You can quickly and easily specify which elements of your 'source' experiment should be incorporated in the target experiments.

Processing

Once the processing is finished, your resulting data files are fully analyzed and ready to be reviewed.

Unmatched Analysis

ASTRA Main Screen

Building on over 40 years of light scattering research and development, ASTRA 7 includes an unrivaled range of analysis features. ASTRA takes full advantage of modern multicore computers, processing data at blazing speeds and with superior accuracy.

Molar Mass Determination

Determine molar mass and size.

Molar Mass via Light Scattering

Light scattering stands alone in its ability to measure the molar mass of macromolecules in solution both accurately and quickly.
Learn more


Molar Mass via Conventional and Universal Calibration

Build a bridge between legacy conventional and universal calibration results and modern, accurate light scattering analysis. Learn more

Protein Conjugate and Copolymer Analysis

Determine molar mass, size, and relative fractions.

If you have an RI detector, a UV detector, and a light scattering instrument, you can directly measure the molar mass, size, and relative fractions of copolymers and protein conjugates. Learn more

Particles and Number Density

Determine size and number density with just a light scattering detector.

It is possible to measure the size and number density of a sample with just a light scattering instrument. ASTRA supports several sophisticated models to determine the size of a macromolecule based solely on the angular variation in the light scattering intensity.
Learn more

Translational Diffusion and Hydrodynamic Radius

Determine translational diffusion and hydrodynamic radius with dynamic light scattering.

On-Line QELS

With the support of a QELS instrument module, you can extend your data analysis to determine the translational diffusion and corresponding hydrodynamic radius of your samples. Learn more


QELS batch Regularization and Cumulants

Determine translational diffusion and corresponding hydrodynamic radius of your unfractionated samples. Learn more

Viscometry

Measure viscosity directly using the Wyatt ViscoStar.

ASTRA provides several analysis features that take advantage of viscosity data. Use these features to further quantify your macromolecules to determine Mark-Houwink-Sakurada terms, hydrodynamic radius, and more. Learn more

Molecular Conformation

Assess the shape of your molecules.

The shape of molecules can be assessed from the molar mass and rms radius of the sample. Learn more

Branching

Determine polymer branching from light scattering, viscosity, or both.

ASTRA includes sophisticated branching calculations to assess the branching characteristics of a polymer sample. Learn more

Distributions

Evaluate the distribution of components in a sample.

Sample properties are determined for individual peaks in a chromatography run. ASTRA also supports visualizing these data in terms of the distribution of these measured quantities. This is extremely powerful when assessing how much of a sample falls with a particular range of molar mass or size. Learn more

Second Virial Coefficient (A2) Measurements

Determine macromolecule self-association behavior.

The second virial coefficient (A2) is a measure of macromolecular self-association, and is one of the few parameters that can be used to predict the crystallization properties of a sample. ASTRA allows you to measure this important characteristic. Learn more

dn/dc and UV extinction coefficient determination

Measure the dn/dc and UV extinction coefficients for your novel samples.

While dn/dc and UV extinction coefficients are documented for many existing compounds, they must be experimentally determined for novel samples. ASTRA provides analysis features to measure these critical sample characteristics. Learn more

Band Broadening Correction

Model and account for inter-detector mixing effects.

While most analysis packages force you to live with the undesirable effects of passing a sample through a series of detectors. ASTRA's revolutionary band broadening correction accounts for these effects to give you most accurate results possible. Learn more

Absorbance Correction

Account for samples that absorb light.

If a sample absorbs light at the MALS laser wavelength, molar mass calculations will be skewed due to less light reaching the scattering volume than expected. ASTRA uses the Forward Monitor detector signal available in all Wyatt MALS instruments to calculate the laser attenuation produced by sample absorption and correct the scattering signal levels. In this manner accurate molecular weights are ensured, even in the presence of absorbtion. Learn more...

Blank Subtraction

Flatten out complicated FFF runs.

Certain analysis techniques, such as FFF, cause large changes in refractive index and viscosity measurements as flow rates are modified during the run. This can make it difficult to properly analyze the file, as the RI and viscosity changes due to flow and pressure changes mask those caused by the sample. ASTRA provides a mechanism to subtract a data collection run without sample (a 'blank') from a sample run to reveal the sample-specific signal changes. Learn more

Error Analysis

ASTRA provides a rock-solid scientific foundation for your results.

Wyatt Technology software is written by scientists for scientists. ASTRA was designed from the ground up to properly compute and propagate uncertainties to ensure our results are publication-quality. Learn more

Cutting Edge Technology

Take advantage of Wyatt Technology's patented technologies.

Wyatt Technology is committed to pushing macromolecular characterization to new levels. Consequently, we have a very strong program to develop intellectual property. Many of the algorithms in ASTRA are on the cutting edge of scientific analysis, and are either patented or have patents pending. ASTRA is protected by:

  • U.S. patent numbers: 5,528,366; 6,411,383; 6,651,009; 6,774,994; 7,386,427; 7,911,594;
  • German patent Numbers: 694 33 615.7-08; 603 19 078.2-08;
  • British patents based on European application numbers: EP 0 665 433; EP 1 510 807;
  • Japanese patent Numbers: 3,580,380; 4,439,211; 4,381,914;

and other patents pending.

State of the Art Reporting

Take control of your analysis reporting with ASTRA's custom reporting system. With just a few mouse clicks, adjust which data to display and how it should be formatted. Add your own logo, incorporate some personal notes, or just pare the default reports to focus on what you care about.

Report Designer

Customizable Logo and Summary

Personalize your reports.

Report Options

In addition to the numerical and content selections provided in the Report Designer, ASTRA allows you to specify a logo image, and where it should be displayed. You can specify the title as well as additional summary information or notes that will appear on the report.

Personalized Data Selection

Choose your data. Choose your formatting.
Show your achievements.

Report Data Options

Control the content and presentation of your reports with a few mouse clicks. Any bit of data generated during analysis is now available for you to incorporate into your report, with just a click of the mouse.

Full Numerical Formatting

Choose your display style and precision.

Click on a result, and ASTRA presents a simple, intuitive interface for controlling the numerical precision and display format of the result. Quickly and easily change results from fixed point to scientific notation, increase or decrease the number of significant digits to display, and determine if you wish uncertainties to be displayed as numerical values or percentages.

Formatting Results

Built from the ground up to comply with 21 CFR Part 11, ASTRA 7 with Security Pack makes compliance complete and intuitive.

  21 CFR Part 11 White Paper

Wyatt Software System Requirements

Please see Host Computer Requirements for details of the computer hardware and operating system required by ASTRA.