Light Scattering University
The LSU Experience
LSU is the brainchild of Dr. Philip Wyatt, who wanted to instill in our customers more than just a passing reference to light scattering. The course demystifies light scattering and provides a unique opportunity to dive into your needs and applications with our expert instructors, connect with light scattering users from around the world, and gain a deeper understanding of your instrumentation and software. Over the past 30 years, the class has grown in its curriculum and its scope. It delves into the theory—and practice—of classical and dynamic light scattering through hands-on practice sessions, software exploration in the computer labs, lectures, and individual instruction. Attendees leave with the most up-to-date documentation, software and firmware versions, and tutorials that benefit everyone back home.
LSU not only reinforces concepts from the first installation training visit but also ensures the same level of knowledge and understanding is passed on to the newest users. The gold standard of education from our experienced LSU faculty gives everyone in the lab the right starting point and sets them up with the best foundation possible. In addition, LSU students discover advanced data processing methods and alternative analytical tools that they may not have been aware of and learn how their MALS and DLS data complements information from other techniques they are using in the lab. A LSU credit, inclusive of airfare, accommodations and most meals, is included for most North American customers who purchase one of our light scattering instruments. While you are here, we work you hard but feed you well—at a variety of Santa Barbara restaurants!
During LSU our students meet Dr. Wyatt, who not only gives an acclaimed Light Scattering Instrument Museum tour, but also delivers a great lecture colloquially referred to as the Dead Physicist’s Talk. There is ample time for our students to meet the people who invented the instruments, build the instruments, as well as those who service and support the instruments. By the time LSU ends, there’s no reason to hesitate to ask for help from someone specifically.
In addition to learning from the faculty, the students in each small cohort learn even more from each other. It’s the perfect opportunity to network, share questions or successes, and troubleshoot common problems. We’ve even introduced colleagues to each other who worked on different floors in the same company but had never met! LSU brings together users with a variety of applications, all of whom share a need for the robust macromolecular characterization afforded by light scattering.
Besides an educational opportunity nonpareil, LSU is a unique time for building rapport between Wyatt Technology and its customers. Not only do we have staff who have been at WTC for decades, but we have customers who have used our equipment that long. Friendships transcend jobs and equipment, and help make the LSU experience personal and meaningful, because we don’t just build instruments, we build relationships. We look forward to welcoming you to Santa Barbara—the American Riviera—sometime soon.
LSU was fun, worthwhile and an unforgettable experience. From the first lecture to the closing remarks, it was all enlightened education: well-organized, useful and interesting. I am grateful beyond description. Your group showed me a highly sophisticated and informative technology, state-of-the-art in analytical chemistry.
This is easily one of the most informative training courses I have attended. It was presented in a good format with lectures mixed with hands-on experience, and had enthusiastic, informed instructors!
I could not be happier with the DynaPro Plate Reader II. It operates extremely well! [The Wyatt Representative] is an outstanding representative and has also provided us with a very high level of service. Wyatt really has been an outstanding company to work with.
Just a few words to express my appreciation about the three-day training I just attended in Santa Barbara. I had been told about the kindness and generosity of Wyatt's people, but last week I was able to realize to what extent. The course, the hotel, the meals, the discussions with all of you, everything was great! I must say that I also enjoyed the three extra days of vacation I took to visit Santa Barbara, Solvang, Santa Ynez and Los Olivos. I love your mountains and waterfront. I will never forget this experience.
Thank you for your help, Wyatt Technology has the best customer service of any company with which I have ever done business. My gratitude to you and your colleagues.
Often described by participants as the best instrument user training they have ever attended, Light Scattering University (LSU) is an intensive three-day experience that combines hard work, good food and a friendly, relaxed atmosphere. LSU operates on two main campuses: at Wyatt Technology Headquarters in Santa Barbara, CA, USA, where attendees enjoy the famously comfortable and sunny Santa Barbara climate and at Wyatt Technology Europe in Dernbach, Germany, with easy access from much of Europe.
In Santa Barbara, LSU and DynaLSU are offered every month. In addition, LSU attendees may elect to add a fourth day for extra training on their Eclipse or Calypso instruments, according to our quarterly schedule, and Mobius training is offered as part of DynaLSU once per quarter.
In Dernbach, LSU is offered every month, and DynaLSU is offered every other month. LSU attendees in Dernbach may elect advanced training on the ViscoStar or Eclipse instruments according to the yearly schedule, including theory, installation, operation, and method development. Half the classes are offered in German.
Additional LSU extensions are offered less frequently, such as our French-language LSU in Toulouse, France, and application-specific training at regional User Meetings. For more details, please explore the LSU training dates in Santa Barbara, Germany, and France.
LSU and DynaLSU are designed to get you up and running with your Wyatt instruments as quickly as possible. The syllabus includes light scattering theory and lots of hands-on work with light scattering instruments and ASTRA or DYNAMICS software. You'll also get an introduction to some of the advanced techniques, such as field-flow fractionation and composition-gradient light scattering. Pass the exams and you'll receive a Master of Light Scattering certificate, in Latin or Greek, guaranteed to impress your boss and coworkers!
Many trainees come away from LSU inspired with new ideas for how light scattering can solve some of their analytical challenges. One of the favorite aspects of LSU mentioned by attendees is the opportunity to meet and work with the scientists and engineers behind the products as well as support staff that they usually only speak with over the phone. Another highlight (available only in Santa Barbara) is the Light Scattering Museum tour, led by none other than Dr. Philip Wyatt, inventor and pioneer of MALS detectors.
DynaLSU is a comparable two-day course for users of DynaPro or Mobius instruments. Many Santa Barbara LSU attendees elect to add a fourth day for extra training on their Eclipse, ViscoStar or Calypso instruments. Dernbach attendees may elect advanced training on field flow fractionation, including theory, installation, operation, and method development. For more details, please explore the Light Scattering University training options.
The lessons are a combination of lectures, seminars, hands-on lab work, quizzes, and individual instruction based upon the background and applications of each student. With a low ratio of students per instructor, there is ample time for individual help and small group work.
Students are encouraged to bring their light scattering data files for individual consultations.
Social time is also scheduled to allow the students to learn from each other and to interact with Wyatt staff in a relaxed atmosphere. We encourage all attendees to join the Light Scattering University Alumni group on LinkedIn, and keep in touch!
- The basics and applications of light scattering with practical demo!
- Instrument setup, maintenance, and flow cell cleaning
- Coupling size exclusion chromatography (SEC) with multi-angle light scattering (MALS) to determine the absolute molar mass and size distributions of macromolecules
- How to get the most out of your light scattering data: the ins and outs of ASTRA Software
- Calibration and use of UV and dRI detectors to determine sample concentration
Small group lab practicals
- Analysis of unfractionated samples and generating a Zimm Plots to determine the molar mass, radius, and the second virial coefficient
- Determination of the refractive index increment dn/dc values through on-line and off-line dRI techniques
- Assessing and optimizing the quality of your light scattering data
Beyond the basics
- Measuring size (hydrodynamic radius) with online or batch dynamic light scattering (DLS) methods (WyattQELS, DynaPro and Mobius)
- How to determine polymer conformation, branching, and intrinsic viscosity with SEC-MALS
- Application-specific seminars:
- analysis of conjugated proteins and copolymers
- field-flow fractionation (FFF)
- batch methods
- analysis of protein-protein interactions with composition-gradient MALS (CG-MALS)
- analysis of light-absorbing proteins
- and more!
- The history of light scattering and a tour of the world's most extensive Light Scattering Museum, conducted by Dr. Philip Wyatt, pioneer of MALS technology
DynaLSU and Mobius LSU: the basics
- The basics and applications of dynamic light scattering (DLS) with practical demo
- Instrument setup, maintenance, cuvette/sample cell cleaning, and sample preparation tips
- How to determine the diffusion coefficient, hydrodynamic radius, and polydispersity of an unfractionated, dissolved sample
- Assessing and optimizing the quality of dynamic light scattering data and data interpretation
- How to get the most out of your light scattering data: Using DYNAMICS software to characterize your proteins, polymers and nanoparticles
Small group lab practicals
- Hands-on DLS analysis of unfractionated samples in microwell plates (Plate Reader), batch cuvettes (NanoStar and Mobius), or flow cell (Mobius) and automation of measurements
- Hands-on SLS analysis of unfractionated samples for molar mass determination (Plate Reader and NanoStar)
- Hand-on PALS analysis (Mobius), automated sample delivery and batch analysis
- Assessing and optimizing the quality of your light scattering data
Beyond the basics
- Advanced analysis techniques:
- temperature transitions (unfolding and aggregation)
- intermolecular interactions via A2 (SLS) and kD (DLS)
- Coupling size exclusion chromatography (SEC) with dynamic light scattering and multi-angle light scattering to determine the radius distribution and molar mass of a fractionated sample
- The history of light scattering and a tour of the world's most extensive Light Scattering Museum conducted by Dr. Philip Wyatt
Additional topics covered in Mobius LSU
- The basics and applications of electrophoretic mobility measurements
- How to determine the electrophoretic mobility, zeta potential, net charge, and isoelectric point
- Assessing and optimizing the quality of mobility data and data interpretation
Who should attend
The lessons learned in LSU and DynaLSU will be of tremendous benefit to those individuals who:
- Purchased a new DAWN, miniDAWN, microDAWN, DynaPro, or Mobius light scattering instrument
- Added Eclipse field-flow fractionation or Calypso concentration-gradient system to a MALS setup
- Changed job responsibilities, and will be using a Wyatt instrument for the first time
- Have been using a Wyatt MALS, DLS, or PALS instrument and want to increase their knowledge of light scattering, data analyses, and applications
- Are responsible for finding new applications for light scattering within their research group