Wyatt Technology

DYNAMICS

DYNAMICS

The intuitive software for acquiring and analyzing size, molar mass and zeta potential or charge with your Möbiuζ, DynaPro Plate Reader III, and DynaPro NanoStar.

Collect, display and analyze your batch light scattering data from Dynamic Light Scattering (DLS), Phase Analysis Light Scattering (PALS), and Static Light Scattering (SLS) measurements. Set up and walk away to perform high-throughput DLS screening in microwell plates or automated mobility and charge measurements using an autosampler.

DYNAMICS offers an optional 21CFR(11)-compliant security and database package for use under GMP and GLP regulatory regimes.

Analyze protein and particle sizes distributions without fractionation
  • First, the basics: Size and size distributions by Cumulants and Regularization for DLS data, cumulative size distribution, as well as molar mass determination for SLS data from the NanoStar. Statistical analysis on your measurements with display of mean, minimum, maximum, and standard deviation is provided. But there is more to your results than calculation of size: DYNAMICS can perform virtually any transition analysis, be it temperature or concentration dependence or any parameter measured or defined by the user. Linear, exponential and sigmoid curve analyses are provided for maximum flexibility.

    Size and Size Distributions

    Analyze protein and particle sizes using the method of Cumulants or Regularization. Report average radius, diameter and diffusion coefficient as well as the width of the distribution in terms of polydispersity, % polydispersity or polydispersity index (PDI). In Regularization, size distributions may be viewed as Intensity or Mass distributions and custom data labels may created for each peak.

      

    Cumulative Size Distributions

    For a broad size distribution, it is important to control the entire distribution rather than the mean radius alone. DYNAMICS provides calculation of the percentage of samples below a certain size of particles (either diameter or radius), with values for D10, D50 and D90 (the size of the sample below 10%, 50% and 90% of the entire distribution lies, respectively). The width of the distribution, defined as (D90-D10)/D50, is calculated as well.

    Transition Analysis

    Monitor protein melting or unfolding. Determine temperature transitions for your biomolecule. Every molecule behaves differently. That's why DYNAMICS provides analysis in terms of the onset transition temperature for aggregating samples, midpoint for samples with a defined end state, and linear analysis for any type of transition.

    Similarly, determine the Critical Micelle Concentration (CMC) with our Concentration Dependence analysis. There are no limitations when it comes to analyzing trends for your samples. Any transition can be analyzed with the Custom Parametric Analysis. Select parameters such as pH versus radius or salt concentration versus molar mass.

    Even perform statistical analysis for duplicate or triplicate samples, or overlay the analysis curves for multiple samples for all of the analysis types listed above.

      

      

    Absolute Molar Mass Determination

    Measure the absolute molar mass of proteins and small polymers in batch with the dedicated static light scattering detector of the DynaPro NanoStar – simultaneously – while collecting DLS data. Compare the results for molar mass with the hydrodynamic radius to determine molecular conformation and distinguish between aggregation and protein unfolding.

    For measurements of the absolute molar mass in chromatography mode or batch measurements of large polymers, we recommend the use of our multi-angle light scattering instruments.

      

  • Everything is already set up for the protein scientist. Simply open a new experiment and start recording your data immediately. Data are stored, analyzed and presented in real time. DYNAMICS features an intuitive 32-bit multi-document graphical user interface. Logically organized, software features are dependent on your specific instrument, showing only the needed features and parameters. At the same time, the software becomes more complex at the right time and situation for data collection, analysis, and presentation. Expandable nodes expose the right level of detail for each measurement. For ease of use, simply click into the appropriate location to bring up desired options in addition to using standard menu items.

      

    Original Data

    Never lose any original data through preprocessing. All acquired autocorrelation functions are stored and can be readily reprocessed using different, customizable data filters to determine the size distribution and other quantities of interest of your samples. Any processing parameter may be changed post-acquisition.

      

    Automated Data Collection

    Unattended operation of your instrument is made possible by using powerful and flexible Event Schedules. Design virtually any sequence of data collection for your instrument, be it temperature or stability studies, plate reader scans or any variation in collection parameters. Wyatt provides an extensive library of Event schedules in the Customer Support Center.

    Extensive Solvent Database

    Over 200 of the most common solvents and buffers are already included in our standard database. Of course, custom solvents may be added as well.

    Automation Interface

    An OLE interface is provided for the high-throughput lab using the DynaPro Plate Reader II. Interface your instrument with a robotic handler for fully automated plate loading and data collection. This email address is being protected from spambots. You need JavaScript enabled to view it. for details.

  • Everything is logically organized: View your results as a table, correlation graph, size distribution or parametric graph with multiple y-axis support. Expandable nodes expose the right level of detail for your measurements, so you can choose to display individual acquisitions, measurements or to overlay complete sets of data.

      

    Flexibility

    Any choice to display parameters in tables and graphs as well as user selectable units and precision are available. Settings can be saved globally or individually as presets (Standard Operating Procedures). Graph settings are customizable with custom axis displays, ranges, fonts and user-defined peak labeling. Overlay views for autocorrelation functions and sized distributions show reproducibility of repeat measurements or changes in your sample.

      

    Fully Customizable

    Add custom solvents and parameters to your data. Custom parameters may be even entered directly in your data table. Even better: Make those a part of your measurement name with the handy Names function!

    Variable Data Filter

    Outliers due to dust may be filtered out by a number of criteria. Apply your filter to all data, selected data, or even manually mark out individual acquisitions. Filtration criteria may be changed at any time.

      

    Data Export and Reporting

    Any display can be exported as a graph or as original data with a simple click of the mouse or by pressing shortcut keys. Results reports are partially customizable.

  • Wyatt Software System Requirements

    Please see Host Computer Requirements for details of the computer hardware and operating system required by DYNAMICS.

   

For essential macromolecular and nanoparticle characterization—The Solution is Light™

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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