First, the basics: Size and size distributions by Cumulants and Regularization for DLS data, cumulative size distribution, as well as molar mass determination for SLS data from the NanoStar. Statistical analysis on your measurements with display of mean, minimum, maximum, and standard deviation is provided. But there is more to your results than calculation of size: DYNAMICS can perform virtually any transition analysis, be it temperature or concentration dependence or any parameter measured or defined by the user. Linear, exponential and sigmoid curve analyses are provided for maximum flexibility.
Size and Size Distributions
Analyze protein and particle sizes using the method of Cumulants or Regularization. Report average radius, diameter and diffusion coefficient as well as the width of the distribution in terms of polydispersity, % polydispersity or polydispersity index (PDI). In Regularization, size distributions may be viewed as Intensity or Mass distributions and custom data labels may created for each peak.
Cumulative Size Distributions
For a broad size distribution, it is important to control the entire distribution rather than the mean radius alone. DYNAMICS provides calculation of the percentage of samples below a certain size of particles (either diameter or radius), with values for D10, D50 and D90 (the size of the sample below 10%, 50% and 90% of the entire distribution lies, respectively). The width of the distribution, defined as (D90-D10)/D50, is calculated as well.
Monitor protein melting or unfolding. Determine temperature transitions for your biomolecule. Every molecule behaves differently. That's why DYNAMICS provides analysis in terms of the onset transition temperature for aggregating samples, midpoint for samples with a defined end state, and linear analysis for any type of transition.
Similarly, determine the Critical Micelle Concentration (CMC) with our Concentration Dependence analysis. There are no limitations when it comes to analyzing trends for your samples. Any transition can be analyzed with the Custom Parametric Analysis. Select parameters such as pH versus radius or salt concentration versus molar mass.
Even perform statistical analysis for duplicate or triplicate samples, or overlay the analysis curves for multiple samples for all of the analysis types listed above.
Absolute Molar Mass Determination
Measure the absolute molar mass of proteins and small polymers in batch with the dedicated static light scattering detector of the DynaPro NanoStar – simultaneously – while collecting DLS data. Compare the results for molar mass with the hydrodynamic radius to determine molecular conformation and distinguish between aggregation and protein unfolding.
For measurements of the absolute molar mass in chromatography mode or batch measurements of large polymers, we recommend the use of our multi-angle light scattering instruments.