Conceptually, the Calypso II is 'just' a box with some syringe pumps and tubing, right? Well, that's what we thought it would be - until coming up against the juxtaposition of challenging biomolecular interaction research and the demands of exquisitely sensitive light scattering instruments. In order to simultaneously achieve:
minimal sample consumption
compatibility from peptides to to high-concentration monoclonal antibodies to nanogels
affinities ranging from pM to mM
while still providing exceptional ease-of-use, accuracy, robustness and reasonable cost in capital and consumables, we had to stretch the limits of available technology.
We worked hard, so you don't have to
We left no stone unturned looking for the rare components that met all our demands, such as one-of-a-kind syringe pumps and pressure transducers as well as hard-to-find, 30 nm pore size, filter membranes and low-volume, high-efficiency degassers. What we couldn't buy, we innovated and designed from scratch.
Users have been particularly pleased with our unique, ultra-low-dead-volume, finger-tight filter housings, which help ensure sufficient solution cleanliness for high-affinity analyses yet retain very little sample. Special valves and syringes were co-developed with suppliers to push syringe pump performance and ease-of-use to the max.
Recognizing that CG-MALS is unfamiliar territory for most users, we've also developed Tutorials, Application Guides, Method Templates, and additional support literature and training to help you get started and keep you on track. Make the most of this amazingly powerful technique - we're here to help at every turn.
Some additional Calypso II features
The layout and design were fine-tuned to reduce sample use while enhancing automation and maintaining compatibility with the DAWN HELEOS II and miniDAWN TREOS, the ultimate in MALS detectors. Special features of the Calypso II include:
Most tubing is 0.010" (0.25 mm) i.d. PEEK for biocompatibility and low volume. May be changed to PTFE or stainless steel for non-aqueous solutions, and larger i.d. for viscous solutions
A range of syringe sizes to meet specific needs
In-line degassing of samples and buffers
Easy access to all tubing and filters, for rapid maintenance
Pressure warnings indicate when a filter membrane is saturated or a capillary tube clogged