Binding of Thrombin-α and an Anti-Thrombin Antibody

Antibody-antigen binding, hormone-receptor interactions, and many other common biomolecular interactions occur at stoichiometries other than 1:1. The Wyatt Calypso^® system utilizes composition-gradient multi-angle light scattering (CG-MALS) to determine both the affinity and binding stoichiometry for macromolecules in solution with no need for immobilization or tagging, which may influence the interaction.