Upcoming Live Webinars
Characterizing Protein-Nucleic Acid Interactions by Light Scattering
Join us for a webinar on August 21 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
Harnessing the interactions between DNA, RNA, and proteins holds much promise for detecting biomarkers, diagnosing disease, and improving cancer-targeting therapeutics. Quantifying these interactions is essential for understanding and controlling their biomolecular mechanisms. Multi-angle light scattering (MALS) is a powerful tool for directly measuring molar masses of proteins, nucleic acids, and complexes in solution without fluorescent or radio labeling.
In this webcast, an expert will present two complementary light-scattering techniques for determining the stoichiometry and affinity of interactions between proteins and nucleic acids:
- Size-exclusion chromatography coupled with MALS, ultraviolet, and differential refractive index detection (SEC–MALS) analyzes each of the species present in a solution of macromolecules
- Composition-gradient MALS (CG–MALS) quantifies the binding affinity and stoichiometry of biomolecular complexes, including multi-step reactions, label-free, and immobilization-free
Application examples will focus on protein-DNA and protein-RNA complexes.
A Quick Guide to Properly Measuring Zeta Potential and Particle Size for Challenging Samples
Join us for a webinar on September 25 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST
Dr. Jason Lin has extensive experience characterizing many types of analytes using light scattering, including some that have been quite challenging.
In this webinar he will present methods and techniques that can be used to overcome common challenges when measuring zeta potential and particle size.
The webinar will cover two main light scattering techniques: dynamic light scattering (DLS) and phase analysis light scattering (PALS). Each technique will be introduced in terms of theory and instrumentation setup. This will be followed with descriptions of common challenges that can arise and specific solutions to overcome these challenges.
Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:
- Development of a New Online Micro-Viscometer for APC and UHPLC Applications
- Crystallized Biotherapeutics: Optimizing mAb Crystallization using Light Scattering
- Separation and Characterization of Complex (Bio)macromolecular Architectures
- Understanding monoclonal antibody unfolding and aggregation
- Applications of Light Scattering to HIV Integrase Structural Biology and Drug Discovery
- µSEC-MALS adds Multi-Angle Light Scattering to UHP-SEC for Measurement of Absolute Molecular Weight and Size
- Applications of Analytical Light Scattering in a Biophysics Core Facility
- Quickly Screen Peptides, Proteins and Nanoparticles for Size and Aggregation Using Advanced Methods
- Advances in High-Throughput Screening of Aggregation, Stability and Viscosity Utilizing Light Scattering in Microwell Plates
- Absolute Characterization of Polymeric Excipients with Size Exclusion Chromatography and Light Scattering
- Absolute Characterization of Glycoproteins and their Interactions with Proteins and Antibodies by Light Scattering
- Essential Polymer Characterization with MALS and FFF
- Small, but bright: µSEC-MALS adds light scattering to UHP-SEC for biophysical characterization of biotherapeutics
- Characterizing Molecular Structure in Synthetic and Natural Polymers by Multi-Angle Light Scattering
- Innovations and Applications of UHP-SEC and Multi-Angle Light Scattering (MALS) for the Analysis of Biotherapeutic Proteins
- Advances in online viscometry: extending the limits of detection for intrinsic viscosity and hydrodynamic radius
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