Wyatt Technology

Upcoming Live Webinars

Probing Submicron Protein Aggregation Using Asymmetrical flow Field-Flow Fractionation (AF4) and Light Scattering

Join us for a webinar on December 11 at 8:00 AM PST | 11:00 AM EST | 17:00 CET

Protein aggregation in biotherapeutic drugs is a major concern as aggregates affect the effective dosage and may cause immunogenic responses in patients. However, a lack of reliable analytical methods has hindered the quantification of submicrometer (0.1 to 1 mm) protein aggregates and a detailed understanding of their formation kinetics. In this study, a simple asymmetrical flow field-flow fractionation (AF4) method is developed and used to investigate nanometer (<0.1 mm) and submicrometer (0.1-1 mm) aggregates of heat-stressed anti-streptavidin (anti-SA) IgG1. The Lumry-Eyring nucleated polymerization model for non-native protein aggregation is fit to AF4 data and kinetic analysis is performed to identify the mechanism of aggregate formation. This work showcases the importance of a separation technique when studying complex and dynamic systems. Questions about the impact of a separation method on potentially labile analytes will also be addressed.

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Understanding Absolute Stoichiometry of Oligomeric Protein Complexes using SEC-MALS

Join us for a webinar on January 29 at 8:00 AM PST | 11:00 AM EST | 17:00 CET

Nucleosome assembly proteins (Naps) are histone chaperones that interact with histones H2A-H2B and/or H3-H4 in the regulation of chromatin architecture. Determining the stoichiometry of Nap-histone complexes has been challenging due to two major factors: Naps undergo dynamic oligomerization that is influenced by salt concentration; and H3-H4 forms a hetero-tetramer. Using SEC-MALS, researchers have determined the absolute stoichiometry of Nap-histone complexes containing Nap orthologs, a new Nap paralog, and relevant deletion constructs.

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Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

 

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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