Upcoming Live Webinars

Looking at proteins from different angles: The unusual behavior of phospholipase PlaB from Legionella pneumophila

Join us for a webinar on Tuesday, February 22 from 10-11 AM CET

Legionella pneumophila, the causative agent of Legionnaires’ Disease, uses contaminated freshwater to trigger potentially fatal pneumonia. It enters lung macrophages and establishes itself in a protected niche by secreting a plethora of proteins into the host cell. Among these factors is a significant number of phospholipases that damage host cell membranes, manipulate signal transduction by interfering with second messengers and provide nutrients to the bacterium. One of these phospholipases is PlaB, a PLA2-type phospholipase that inserts into the outer membrane of L. pneumophila after being secreted via an unknown pathway. Interestingly, PlaB shows lowest specific activity at high protein concentrations, which is the consequence of tetramer formation and may represent a means of self-protection that prevents harmful activity while PlaB is synthesized within the L. pneumophila cell.

A surprising mechanism that controls the tetramerization of PlaB was discovered by employing different analytical methods, including SEC-MALS and protein crystallography in prominent roles. These findings demonstrate the synergy of looking at proteins from different angles.

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SEC-MALS Characterization of Block Copolymers

Join us for a webinar on Thursday, February 24 at 8:00 AM PST | 11:00 AM EST | 17:00 CET

Block copolymers are of great current interest, as routes to self-assembled nanostructures in solution and in bulk. Advances in controlled synthetic methodologies have greatly expanded access to such materials, yet routine characterization techniques are in some aspects inadequate. Block copolymers present particular challenges for characterization by size-exclusion chromatography (SEC), which will be identified and discussed. This webinar will review how adding detection by multi-angle light scattering (SEC-MALS) can solve some of these issues as well as the remaining difficulties.

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Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

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