Upcoming Live Webinars
Probing Submicron Protein Aggregation Using Asymmetrical flow Field-Flow Fractionation (AF4) and Light Scattering
Join us for a webinar on December 11 at 8:00 AM PST | 11:00 AM EST | 17:00 CET
Protein aggregation in biotherapeutic drugs is a major concern as aggregates affect the effective dosage and may cause immunogenic responses in patients. However, a lack of reliable analytical methods has hindered the quantification of submicrometer (0.1 to 1 mm) protein aggregates and a detailed understanding of their formation kinetics. In this study, a simple asymmetrical flow field-flow fractionation (AF4) method is developed and used to investigate nanometer (<0.1 mm) and submicrometer (0.1-1 mm) aggregates of heat-stressed anti-streptavidin (anti-SA) IgG1. The Lumry-Eyring nucleated polymerization model for non-native protein aggregation is fit to AF4 data and kinetic analysis is performed to identify the mechanism of aggregate formation. This work showcases the importance of a separation technique when studying complex and dynamic systems. Questions about the impact of a separation method on potentially labile analytes will also be addressed.
Understanding Absolute Stoichiometry of Oligomeric Protein Complexes using SEC-MALS
Join us for a webinar on January 29 at 8:00 AM PST | 11:00 AM EST | 17:00 CET
Nucleosome assembly proteins (Naps) are histone chaperones that interact with histones H2A-H2B and/or H3-H4 in the regulation of chromatin architecture. Determining the stoichiometry of Nap-histone complexes has been challenging due to two major factors: Naps undergo dynamic oligomerization that is influenced by salt concentration; and H3-H4 forms a hetero-tetramer. Using SEC-MALS, researchers have determined the absolute stoichiometry of Nap-histone complexes containing Nap orthologs, a new Nap paralog, and relevant deletion constructs.
Recent Webinars
Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:
- The Importance of Light Scattering in Biopharmaceutical Formulation Development
- Predicting and Evaluating the Stability of Therapeutic Protein Formulations by Dynamic Light Scattering and Machine Learning
- A Quick Guide to Properly Measuring Zeta Potential and Particle Size for Challenging Samples
- Characterizing Protein-Nucleic Acid Interactions by Light Scattering
- FFF 2.0: Obtaining high resolution distributions of nanoparticle size and zeta potential
- Development of a New Online Micro-Viscometer for APC and UHPLC Applications
- Crystallized Biotherapeutics: Optimizing mAb Crystallization using Light Scattering
- Separation and Characterization of Complex (Bio)macromolecular Architectures
- Understanding monoclonal antibody unfolding and aggregation
- Applications of Light Scattering to HIV Integrase Structural Biology and Drug Discovery
- µSEC-MALS adds Multi-Angle Light Scattering to UHP-SEC for Measurement of Absolute Molecular Weight and Size
- Applications of Analytical Light Scattering in a Biophysics Core Facility
- Quickly Screen Peptides, Proteins and Nanoparticles for Size and Aggregation Using Advanced Methods
- Advances in High-Throughput Screening of Aggregation, Stability and Viscosity Utilizing Light Scattering in Microwell Plates
- Absolute Characterization of Polymeric Excipients with Size Exclusion Chromatography and Light Scattering
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