Wyatt Technology

Upcoming Live Webinars

Biophysical studies of HIV integrase: structural biology, drug discovery and mechanisms of action

Join us for a webinar on February 27 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

We apply multiple biophysical techniques to the study of HIV integrase and the antiviral effects of small molecules that target this protein, including X-ray crystallography, small angle X-ray scattering, and analytical ultracentrifugation. Light scattering measurements, including SEC-MALS and DLS, are key enablers of this ongoing research. In the webinar I will describe how all of these techniques come together and complement each other to provide deeper understanding of the structure and function of HIV integrase, more effective screening of anti-HIV integrase compounds, and a complete picture of the therapeutic mechanism of arising drug candidates.

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Understanding monoclonal antibody unfolding and aggregation

Join us for a webinar on March 14 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

Investigations on thermal behavior are essential during the development of therapeutic proteins. Understanding the link between thermal unfolding and aggregation might help to minimize conformational and colloidal instabilities. In this study, a therapeutic monoclonal antibody (MAb) and its Fab and Fc fragments were investigated by differential scanning fluorimetry, temperature-ramped dynamic light scattering and turbidity measurement. Repulsive net charges at the low pH increased the colloidal stability, although a reduction of the conformational stability was observed. Aggregation was driven by hydrophobic interactions. Thus, the presented methods described and explained the thermal behavior of the protein and demonstrated their value for the development of pharmaceutical protein products. Coupling of differently charged hydrophobic payloads affected colloidal stability mostly based on the charge effects. The combination of orthogonal methods allows a better understanding of the conformational and colloidal stability of mAbs and mAb-conjugates.

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Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

 

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Ultimate Guide

Thirteen key questions to ask when buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument.

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Our Solutions Catalog combines Wyatt's product lines, applications and services into a single comprehensive booklet.

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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