Upcoming Live Webinars

Predicting and evaluating the stability of therapeutic protein formulations by dynamic light scattering and machine learning

Join us for a webinar on October 23 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

PIPPI, which stands for Protein-excipient Interactions and Protein-Protein Interactions, is a European academic-industrial consortium addressing the challenges in formulation of protein-based drugs. Its overall objective is to develop methodologies, tools and databases to guide the rational formulation of robust protein-based therapeutics. Much of the effort carried out under PIPPI has utilized dynamic light scattering (DLS), the primary biophysical technique for screening aggregation in a high-throughput fashion.

Thanks to efficient utilization of resources and high flexibility, DLS can be applied in multiple ways to increase the progression of new-drug candidates into phase 1 clinical trial. In this webinar a series of DLS methods are presented for evaluating stability under different formulations, including standard protocols and the latest applications. All, if not most, of these methods are carried out in high-throughput fashion using convenient microwell plates in order to test a large variety of candidates and formulation conditions.

Due to the overwhelming amount of data available from high throughput techiniques such as plate-based DLS, there is a staggering increase in opportunities for finding patterns in data. Such a patterns are helpful in ab initio prediction of stability-indicating parameters. A machine learning application developed to identify those patterns, and trained using a standard formulation screening dataset, is shown.

The Importance of Light Scattering in Biopharmaceutical Formulation Development

Join us for a webinar on November 15 at 8:00 AM PDT | 11:00 AM EDT | 17:00 CEST

Protein formulation development and characterization relies heavily on a set of analytical tools and techniques to accurately assess a molecule’s stability. This toolbox includes a standard set of tools which has come to us through industry wisdom and experience.

Light scattering is increasingly considered a “standard” tool in the set, and it is a core part of Fujifilm’s approach to protein formulation development. We will introduce how various types of light scattering measurements are used in our work and offer several case studies where light scattering data impacted the project direction or was the only way to accurately measure the product.

Recent Webinars

Previous webinars may be viewed in our Webinar Library. The following recent webinars are now available:

• A Quick Guide to Properly Measuring Zeta Potential and Particle Size for Challenging Samples
• Characterizing Protein-Nucleic Acid Interactions by Light Scattering
• FFF 2.0: Obtaining high resolution distributions of nanoparticle size and zeta potential
• Development of a New Online Micro-Viscometer for APC and UHPLC Applications
• Crystallized Biotherapeutics: Optimizing mAb Crystallization using Light Scattering
• Separation and Characterization of Complex (Bio)macromolecular Architectures
• Understanding monoclonal antibody unfolding and aggregation
• Applications of Light Scattering to HIV Integrase Structural Biology and Drug Discovery
• µSEC-MALS adds Multi-Angle Light Scattering to UHP-SEC for Measurement of Absolute Molecular Weight and Size
• Applications of Analytical Light Scattering in a Biophysics Core Facility
• Quickly Screen Peptides, Proteins and Nanoparticles for Size and Aggregation Using Advanced Methods
• Advances in High-Throughput Screening of Aggregation, Stability and Viscosity Utilizing Light Scattering in Microwell Plates
• Absolute Characterization of Polymeric Excipients with Size Exclusion Chromatography and Light Scattering

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