Not sure which instruments and accessories you need to carry out any of the light scattering techniques we provide? Visit the Instrument Overview to find the right configuration, where we have listed each technique along with the primary instruments and associated accessories.
If you know which type of instrument interests you, the categories below will help you compare models.
Couple a DAWN®, miniDAWN® or μDAWN® multi-angle light scattering (MALS) detector to SEC or FFF for reliable characterization of distributions of molar mass / molecular weight, size, conjugation and conformation of macromolecules, aggregates, fragments, conjugates, colloids, and nanoparticles.
All measurements take place in solution, and are independent of inherently unreliable column calibration.compare
Dynamic light scattering (DLS) measures the hydrodynamic radius of proteins, aggregates, nanoparticles, vesicles, and more. The NanoStar® uses standard microcuvette format and the DynaPro® Plate Reader III high-throughput microwell plates.
The Möbiuζ® measures electrophoretic light scattering (ELS) and DLS simultaneously to determine zeta potential or molecular charge, automated by an autosampler or manually, under a wide range of solvents.compare
Optilab differential refractive index (dRI) detectors are utilized primarily for on-line measurement of analyte concentration in SEC-MALS and FFF-MALS, and for determination of analyte dn/dc (refractive index increment), and the absolute refractive index (aRI) of solvents.
Optilab® dRI detectors serve as universal concentration detectors since no chromaphore or fluorophore is necessary. Required for Conjugation Analysis.
The ViscoStar® III on-line differential viscometer measures the intrinsic viscosity of polymers downstream of SEC. Differential viscometry is best combined with MALS and RI for analysis of polymer conformation and Mark-Houwink-Sakurada parameters.
On its own, the ViscoStar can determination hydrodynamic radius, or may be utilized with the Universal Calibration technique to assess polymer molar mass / molecular weight over a wide range.read more
The Calypso® II automated composition-gradient accessory and software are used in conjunction with a MALS detector (CG-MALS) to characterize biomolecular interactions, label-free and immobilization-free. CG-MALS determines equilibrium dissociation constants self- and hetero-association as well as more complex interactions.
Also useful for automation of Zimm plots, dn/dc analysis, reaction kinetics and polymer interaction analyses.read more
Eclipse® devices carry out field-flow fractionation (FFF) of macromolecules and nanoparticles ranging in size from 1 nm - 1000 nm, with no stationary phase, eliminating shear and non-ideal column packing interactions. Integrates with top-quality HPLC pumps and autosamplers.
A wide choice of flat and hollow-fiber channels separates both soluble and colloidal components, from nanogram to milligram quantities.compare
Our comprehensive software solution for MALS and DLS analysis in chromatography, FFF or batch mode.
ASTRA® is included with each MALS, dRI and dVI detector. It is available in both standard and 21 CFR(11)-compliant versions, and offers additional modules for advanced capabilities, such as universal calibration (online viscometry), particle analysis or research database.read more
Software for batch DLS, SLS and ELS measurements in the DynaPro® and Möbiuζ® instruments, included with each.
DYNAMICS® calculates size, size distributions, and derived parameters such as the melting temperature TM, the aggregation onset temperature Tonset and the concentration coefficient of diffusion kD.
Design, control, data acquisition and analysis of CG-MALS measurements for macromolecular interactions in solution.
CALYPSO provides an unusually extensive set of interaction models covering self-association, hetero-association, and non-specific interactions with advanced modeling capabilities.
Controls the Eclipse® DualTec™ and AF4 Field-Flow Fractionation (FFF) systems via standard industry-wide packages such as ChemStation, OpenLAB, Chromeleon and LCsolution.
Coupled with ASTRA for data collection and analysis, ECLIPSE provides users with a turn-key solution for macromolecular and nanoparticle characterization.
When you purchase a new Wyatt instrument, you get more than a warranty contract - we are here to help with a friendly, expert staff of technicians and Ph.D. scientists who provide industry-benchmark training, technical and application assistance, instrument maintenance and more.
Not ready to buy a Wyatt instrument but need the assistance of the foremost experts in light scattering analysis? Consider our analytical lab and consulting services in the areas of macromolecular and nanoparticle characterization, including SEC-MALS, FFF-MALS, CG-MALS, DLS and ELS.