Couple a DAWN®, miniDAWN® or microDAWN™ multi-angle light scattering (MALS) detector to SEC or FFF for reliable characterization of distributions of molar mass / molecular weight, size, conjugation and conformation of macromolecules, aggregates, fragments, conjugates, colloids, and nanoparticles.
All measurements take place in solution, and are independent of inherently unreliable column calibration.
Dynamic light scattering (DLS) measures the hydrodynamic radius of proteins, aggregates, nanoparticles, vesicles, and more. The NanoStar® uses standard microcuvette format and the DynaPro® Plate Reader high-throughput microwell plates.
The Mobius measures electrophoretic light scattering (ELS) and DLS simultaneously to determine zeta potential or molecular charge, automated by an autosampler or manually, under a wide range of solvents.
Optilab and microOptilab™ refractive index (dRI) detectors are utilized primarily for on-line measurement of analyte concentration in SEC-MALS and FFF-MALS, and for determination of analyte dn/dc (refractive index increment), and the absolute refractive index (aRI) of solvents.
Optilab dRI detectors serve as universal concentration detectors since no chromaphore or fluorophore is necessary. Required for Conjugation Analysis.
The ViscoStar® and microViscoStar™ on-line differential viscometers measure the intrinsic viscosity of polymers downstream of SEC. Differential viscometry is best combined with MALS and RI for analysis of polymer conformation and Mark-Houwink parameters.
On its own, the ViscoStar may be utilized with the Universal Calibration technique or Mark-Houwink parameters to assess polymer molar mass / molecular weight over a wide range.
Eclipse® devices carry out field-flow fractionation (FFF) of macromolecules and nanoparticles ranging in size from 1 nm - 1000 nm, with no stationary phase, eliminating shear and non-ideal column packing interactions. Integrates with top-quality HPLC pumps and autosamplers.
A wide choice of flat and hollow-fiber channels separates both soluble and colloidal components, from nanogram to milligram quantities.
The Calypso® automated composition-gradient accessory and software are used in conjunction with a MALS detector (CG-MALS) to characterize biomolecular interactions, label-free and immobilization-free. CG-MALS determines equilibrium dissociation constants self- and hetero-association as well as more complex interactions.
Also useful for automation of Zimm plots, dn/dc analysis, reaction kinetics and polymer interaction analyses.
Our comprehensive software solution for MALS and DLS analysis in chromatography, FFF or batch mode.
ASTRA® is available in both standard and 21 CFR(11)-compliant versions, and offers additional modules for advanced capabilities, such as universal calibration (online viscometry), particle analysis or research database.
Control the Eclipse® DualTec™ and AF4 Field-Flow Fractionation (FFF) systems directly via standard industry-wide packages such as ChemStation, OpenLAB, Chromeleon and LCsolution, or use the native Voyager CDS®.
Coupled with ASTRA for data collection and analysis, ECLIPSE provides users with a turn-key solution for macromolecular and nanoparticle characterization.