DynaPro Plate Reader

DynaPro Plate Reader

High-throughput, automated, Dynamic and Static Light Scattering

REVOLUTIONIZE

Revolutionize your performance by replacing your conventional, cuvette-based dynamic light scattering unit with the automated DynaPro® Plate Reader III. The DynaPro excels at high-throughput sizing and screening of pharmaceutical and nanoparticle formulations to enable novel workflows that simply would not be possible with other DLS instruments. Even if you need to run just a handful of samples, it's a great productivity booster.


COMPREHENSIVE SOLUTION

For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (Tonset, Tm, Tagg) and colloidal stability (A2/B22, kD) may be quantified with plate-based DLS and SLS. Even viscosity of concentrated protein solutions can be screened across dozens of formulations in a single plate.


COMPATIBLE WITH DYNAMICS

The DynaPro Plate Reader is accompanied by DYNAMICS, our powerful software package for analyzing automated batch dynamic light scattering. The Security Pack (DYNAMICS SP) option provides 21 CFR (11) compliance for GMP/GLP use.

Revolutionize DLS

The DynaPro Plate Reader offers dynamic light scattering (DLS) and static light scattering (SLS) measurements of the size, molecular weight and interactions of proteins, nanoparticles and other macromolecules in situ in industry-standard microwell plates. No liquid handling occurs after dispensing into the plates, to maximize throughput and minimize sample carryover. This ground-breaking instrument provides rapid optimization of the formulation process for:

A wealth of applications

The DynaPro Plate Reader provides unparalleled levels of ease-of-use, productivity, reproducibility, and flexibility in order to address additional demanding applications such as:

  • Assessing aggregation or oligomerization
  • Quantifying AAV titer for multiple processes
  • Determining protein stability-indicating parameters such as thermal denaturation and colloidal stability
  • Optimizing protein crystallization
  • Screening high-concentration protein solutions for reversible association and viscosity

Key features

  • Measure hydrodynamic radius from 0.5 nm to 1000 nm
  • Sensitivity for size down to 0.125 mg/mL lysozyme
  • Measure weight-average molar mass from 1 to 1000 kDa
  • Industry standard microwell plates of 96, 384 or 1536 wells
  • Sample volume from 4 μL to 150 μL
  • All measurements made in situ in the wells
  • Temperature ramps from 4°C to 85°C
  • Integrates with plate-based liquid handling robotics

See how easy it is to make measurements with the DynaPro Plate Reader,
courtesy of Yale University Chemical and Biophysical Instrumentation Center.

A wealth of features

  • Rapid or slow temperature ramps 4°C to 85°C with excellent uniformity across the plates. Samples are heated in parallel and all filled wells measured at each temperature.
  • Well read times as short as 5 to 10 seconds
  • Seal wells with oil or sealing tape to prevent evaporation. The tape is gently heated to prevent condensation.
  • Integration with liquid-handling robots for higher levels of automation, especially when your workflow is plate-based, and utilizes spectroscopic and other plate readers in addition to DLS/SLS
  • A camera for viewing each well from below, to aid in data diagnostics (e.g. to detect precipitation or bubble formation)

A set of images collected by the onboard camera, showing clean wells and wells containing bubbles, crystals and precipitates.

Lab automation

An OLE software API is available for interfacing the DynaPro Plate Reader to robotic liquid handling in the high-throughput lab. This capability allows for fully automated plate loading and data collection.

Wyatt partners with several companies that offer integrated solutions for automated loading and analysis of plated samples: 


Hudson Robotics Plate Transfer

Click to play video

   Contact Hudson Robotics

In addition, Hamilton Robotics, Caliper Life Sciences, and Tecan offer custom integration services.

DYNAMICS - Automates well-plate scans, temperature ramps, etc. and analyzes DLS data to determine size distributions, temperature onset of melting or aggregation, second virial coefficient A2and the diffusion interaction parameter kD.

DYNAMICS is offered with a 21CFR(11)-compliant Security Pack enabling the use of the DynaPro Plate Reader in a GMP environment.

Supported Well Plate Formats 96, 384 or 1536 *
Dynamic Light Scattering
   Size Range (Radius, Rh) 0.5 to 1000 nm
   Minimum Concentration @ 14 kDa 0.125 mg/mL
Static Light Scattering
   Molar Mass Range 1000 to 1,000,000 Da
   Minimum Concentration @ 67 kDa 1 mg/mL ††
Optics
   Laser Wavelength 830 nm
   Laser Power Control Programmable 10% – 100%
   Attenuation Range 1 to 105
Temperature Control 4 to 85°C †††
Fluidics
   Minimum Sample Volume 4 µL ††††
Data Acquisition Time 1 to 3600 seconds per well per read
Read Time per Well 5 – 20 seconds
Electronics
   Correlator 512 channels †††††
   Onboard Camera 3 megapixels ††††††
   Digital Communication Ethernet (TCP/IP)
Dimensions 60 cm (L) x 36 cm (W) x 25 cm (H)
* Supports many industry standard well plates. Please contact support@wyatt.comfor a list of supported plates.

Minimum concentration specified with 50 µL of Lysozyme (14 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

†† Minimum concentration specified with 50 µL of BSA (67 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

††† Accuracy of ± 0.5°C for 4 – 50°C, and ± 1°C for 50 – 80°C. Minimum temperature of 4°C requires a laboratory temperature of 24°C or below.

†††† Minimum volume specified with 2 mg/mL Lysozyme in a 1536 well plate, Wyatt P/N P8803-1536, with 25 seconds acquisition.

††††† 100 nsec sampling time in a multi-tau layout

†††††† Camera operates with sample temperature in the range of 4 – 50°C, and automatically powers off above 50°C.


Host PC requirements may be found in Computer Requirements.

Specifications subject to change without notice.

"Very important to validate proteins before crystallization."
"DLS from Wyatt is very easy to use. Fast and reliable data about your protein solutions. New and updated software allows for easy setup of temp ramping and temp dependent protein aggregation." Michal Boniecki, University of Saskatchewan

"A great addition to any protein structural analysis lab!"
"We just got the DynaPro Plate Reader III and it is a great addition to our lab. Results are of high quality and reproducibility, all with an intuitive and well laid out user interface. The instrument is often used as a pre-cursor to or to consolidate data from SAXS protein analysis. We often work with small concentrations and the plate reader has a high enough sensitivity for our purposes." James Jorgensen, Structural and Biophysical Core Facility

"Very useful instrument for protein quality control"
"The first DLS instrument that enables high-throughput sizing and screening of pharmaceutical and nanoparticle formulations and only requires low sample amount." Chien-hung Chen, Takeda San Diego

"Excellent instrument for characterizing proteins and small molecules."
"We use our plate reader all the time. It's and excellent way for us to characterize the reagents and compounds we are working with. We have also used this to scout buffer conditions during assay development. We find the DynaPro plate reader to be very helpful in our drug discovery efforts." Jonathan Brooks, Pfizer

Other DLS & ELS Instruments

DynaPro NanoStar, molar mass determination, molar mass measurement, molar mass analysis, molecular weight measurement, molar mass characterization

DynaPro® NanoStar® - With sample volumes as small as 1.25 µL and temperature control spanning -15°C to +150°C, the NanoStar goes above and beyond traditional cuvette-based DLS instruments. It offers an optimized static light scattering detector in parallel to the DLS detection system in order to determine true molar mass.

Mobius - The finest, most versatile light scattering instrument for reliable electrophoretic mobility and size measurements of proteins, polymers, and all types of nanoparticles. Wyatt Technology's Mobius is the first and only light scattering instrument that makes reliable, reproducible, and non-destructive electrophoretic mobility measurements of macromolecules as small as 2 nm in radius (as well as micron-size nanoparticles) in most aqueous buffers.