DynaPro Plate Reader

DynaPro Plate Reader

High-throughput, automated, dynamic and static light scattering

Upgrade to plates

Revolutionize your performance by replacing your conventional, cuvette-based dynamic light scattering unit with the automated DynaPro Plate Reader III. The DynaPro excels at high-throughput sizing and screening of pharmaceutical and nanoparticle formulations to enable novel workflows that simply would not be possible with other DLS instruments. Even if you need to run just a handful of samples, it's a great productivity booster.

Comprehensive solution

For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (Tonset, Tm, Tagg) and colloidal stability (A2/B22, kD) may be quantified with plate-based DLS and SLS. Even viscosity of concentrated protein solutions can be screened across dozens of formulations in a single plate.

For nanoparticle formulation and process development, including viral vectors, non-viral vectors and nano-drug delivery vehicles, the DynaPro adds particle concentration / physical titer to basic DLS measurements of size and polydispersity. Its automation is particularly valued to screen fractions during process development and then to quantify CQAs in quality control.

Compatible with DYNAMICS

The DynaPro Plate Reader is operated by DYNAMICS, our powerful software package for collecting, analyzing, and reporting automated batch dynamic and static light scattering data.

DYNAMICS is offered with a 21 CFR Part 11 compliant Security Pack option, enabling the use of the DynaPro Plate Reader in a GMP environment.

Revolutionize DLS

The DynaPro Plate Reader offers dynamic light scattering (DLS) and static light scattering (SLS) measurements of the size, molecular weight, concentration and interactions of proteins, nanoparticles and other macromolecules in situ in industry-standard microwell plates. No liquid handling occurs after dispensing into the plates, to maximize throughput and minimize sample carryover. This ground-breaking instrument provides rapid optimization of the formulation process for:

A wealth of applications

The DynaPro Plate Reader provides unparalleled levels of ease-of-use, productivity, reproducibility, and flexibility in order to address additional demanding applications such as:

  • Assessing aggregation or oligomerization
  • Quantifying AAV titer for multiple processes
  • Determining protein stability-indicating parameters such as thermal denaturation and colloidal stability
  • Optimizing protein crystallization
  • Screening high-concentration protein solutions for reversible association and viscosity

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Key features

  • Measure hydrodynamic radius from 0.5 nm to 1000 nm
  • Determine particle concentration for particles up to 320 nm in diameter
  • Sensitivity for size down to 0.125 mg/mL lysozyme
  • Measure weight-average molar mass from 1 to 1000 kDa
  • Industry-standard microwell plates of 96, 384 or 1536 wells
  • Sample volume from 4 μL to 150 μL
  • All measurements made in situ in the wells
  • Temperature ramps from 4 °C to 85 °C
  • Integrates with plate-based liquid handling robotics

Automated characterization of size, stability and molecular weight in industry-standard microwell plates

Click here to request a copy of our DynaPro Plate Reader brochure.

Introducing the DynaPro Plate Reader III

Step up to automated DLS

A wealth of features

  • Rapid or slow temperature ramps 4 °C to 85 °C with excellent uniformity across the plates. Samples are heated in parallel and all filled wells measured at each temperature.
  • Well read times as short as 5 to 10 seconds
  • Seal wells with oil or sealing tape to prevent evaporation. The tape is gently heated to prevent condensation.
  • Integration with liquid-handling robots for higher levels of automation, especially when your workflow is plate-based, and utilizes spectroscopic and other plate readers in addition to DLS/SLS
  • A camera for viewing each well from below, to aid in data diagnostics (e.g. to detect precipitation or bubble formation)

A set of images collected by the onboard camera, showing clean wells and wells containing bubbles, crystals and precipitates.

Lab automation

An OLE software API is available for interfacing the DynaPro Plate Reader to robotic liquid handling in the high-throughput lab. This capability allows for fully automated plate loading and data collection.

Wyatt partners with several companies that offer integrated solutions for automated loading and analysis of plated samples: 

Hudson Robotics Plate Transfer

Click to play video

Contact Hudson Robotics

In addition, Hamilton Robotics, Caliper Life Sciences, HighRes Biosolutions and Tecan offer custom integration services.

DYNAMICS - Automates well-plate scans, temperature ramps, etc. and analyzes DLS and SLS data to determine size and size distributions, molar mass, temperature onset of melting or aggregation, particle concentration, second virial coefficient A2 and the diffusion interaction parameter kD.

DYNAMICS is offered with a 21 CFR Part 11 compliant Security Pack option, enabling the use of the DynaPro Plate Reader in a GMP environment.

Supported Well Plate Formats 96, 384 or 1536 *
Dynamic Light Scattering
   Size Range (Radius, Rh) 0.5 to 1000 nm
   Minimum Concentration @ 14 kDa 0.125 mg/mL
Static Light Scattering
   Molar Mass Range 1000 Da to 1 MDa ††
   Minimum Concentration @ 67 kDa 1 mg/mL †††
   Laser Wavelength 830 nm
   Laser Power Control Programmable 10% – 100%
   Attenuation Range 1 to 105
Temperature Control 4 to 85 °C ††††
   Minimum Sample Volume 4 µL †††††
Data Acquisition Time 1 to 3600 seconds per well per read
Read Time per Well 5 – 20 seconds
   Correlator 512 channels ††††††
   Onboard Camera 3 megapixels †††††††
   Digital Communication Ethernet (TCP/IP)
Dimensions 60 cm (L) x 36 cm (W) x 25 cm (H)

* Supports many industry standard well plates. Please contact [email protected]for a list of supported plates.

Minimum concentration specified with 50 µL of Lysozyme (14 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

†† Upper limit depends on conformation: it is limited to a maximum Rh of 12 nm.

††† Minimum concentration specified with 50 µL of BSA (67 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

†††† Accuracy of ± 0.5 °C for 4 – 50 °C, and ± 1 °C for 50 – 80°C. Minimum temperature of 4 °C requires a laboratory temperature of 24 °C or below.

††††† Minimum volume specified with 2 mg/mL Lysozyme in a 1536 well plate, Wyatt P/N P8803-1536, with 25 seconds acquisition.

†††††† 100 nsec sampling time in a multi-tau layout

††††††† Camera operates with sample temperature in the range of 4 – 50 °C, and automatically powers off above 50 °C.

Host PC requirements may be found in Computer Requirements.

Specifications subject to change without notice.

Other DLS & ELS instruments

DynaPro NanoStar II - With sample volumes as small as 2 µL and temperature control spanning -10 °C to +120 °C, the NanoStar goes above and beyond traditional cuvette-based DLS instruments. It offers an optimized static light scattering detector in parallel to the DLS detection system in order to determine true molar mass and reliable particle concentrations.

DynaPro ZetaStar - A manual or automated light scattering instrument for analysis of size distribution, particle concentration, molecular weight, turbidity and zeta potential of proteins, nanomedicines, gene vectors, extracellular vesicles, nanoemulsions and polymers. It uniquely measures DLS and ELS simultaneously and can measure zeta potential in salts solutions greater than physiological conditions.

NanoStar, molar mass determination, molar mass measurement, molar mass analysis, molecular weight measurement, molar mass characterization